1.Assignment of CCR7 gene to chicken chromosome 27 by radiation hybrid panel mapping.
Yong TIAN ; Li-zhi LU ; Yan FU ; Zheng-rong TAO ; Jun-da SHEN ; De-qian WANG ; Ai-ping YUAN ; Zhao-zheng YIN
Journal of Zhejiang University. Science. B 2007;8(5):314-317
The protein encoded by CC chemokine receptor 7 (CCR7) is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 (6 cR away) with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH (radiation hydrid) map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.
Animals
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Base Sequence
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Chickens
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genetics
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Chromosomes
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genetics
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Humans
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Molecular Sequence Data
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Radiation Hybrid Mapping
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methods
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Receptors, CCR7
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Receptors, Chemokine
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genetics
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Sequence Analysis, DNA
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methods
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Sequence Homology, Nucleic Acid
2.Cloning and mapping analysis of cDNA fragment OPB7-1 gene in human lung adenocarcinoma.
Hong FAN ; Yu LI ; Yanqiu DENG ; Yingzhun CHEN ; Huichen FENG ; Songbin FU ; Guiyin ZHANG ; Pu LI
Chinese Journal of Medical Genetics 2003;20(2):156-159
OBJECTIVETo search the candidate gene in the development and metastasis of lung adenocarcinoma and shed light on the possible molecular mechanism of the development of lung carcinoma.
METHODSUsing methods of cell culture, reverse transcription-PCR, RH gene mapping and RNA in situ hybridization.
RESULTSThe cDNA fragment named OPB7-1 was mapped at 1p31-1p34 by RH gene mapping method. The fragment sequences obtained from lung cDNA library of normal person and cell line of AGZY83-a were similar in length but showed individual base difference. For OPB7-1, there is a low homogeneity to known gene by analysis in GenBank, but 3 contigs homologous to OPB7-1 were located at chromosome 1(1p31-1p34). Different degrees of expression were noted in tumor tissues from 24 cases of lung carcinoma, however no significant expression was found in their corresponding normal tissues. And high expression was found in the lung tissues of cases with lymph node metastasis.
CONCLUSIONOPB7-1 may be a novel gene. It may be a tumor related gene in occurrence and metastasis of lung carcinoma.
Adenocarcinoma ; genetics ; Animals ; Chromosome Mapping ; Cloning, Molecular ; DNA, Complementary ; genetics ; Gene Expression Regulation, Neoplastic ; Genetic Predisposition to Disease ; genetics ; Humans ; In Situ Hybridization ; Lung Neoplasms ; genetics ; pathology ; RNA, Neoplasm ; genetics ; metabolism ; Radiation Hybrid Mapping ; Rats ; Tumor Cells, Cultured