No abstract available.
Rabies*

80% subjects among 50 bitten by dogs/cats were fully injected 4-6 doses of rabies vaccine. By ELISA (platelia Rage kit of Diagnostic Pasteur), we found that 42.5% of serum samples taken after injection 4 doses of rabies vaccine have titer 0.5IU/ml, it met WHO's minimum requirement. The GMT was 2.71 EU/ml. 62.5% of serum samples taken after injection 6 doses of rabies vaccine have titer 0.5IU/ml. The GMT was 3.14UE/ml.
Vaccines ; Rabies

This is a study on establishing the National References for inactivated rabies vaccine for human produced in ViÖt Nam. The results showed that the ViÖt Nam National References for Rabies Vaccine met the requirements of WHO
Vaccines ; Rabies

Rabies virus

Objectives: To determine the knowledge, attitude, and practices (KAP) on rabies prevention and control among primary care providers of children 0-18 years old. Methodology: This is an analytical cross-sectional study conducted among primary care providers of children 0- 18-years old in Barangay Sambag I, Cebu City, Philippines. A questionnaire to determine the KAP on rabies prevention and control, originally developed by Lañada et al., was modified and contextualized to the local setting. Respondents with at least 75% correct answers per domain were considered to have good overall knowledge, attitude, and practices. Furthermore, each question under the above domains was analyzed separately to determine any gaps in KAP. Results were recorded as frequencies and percentages. The association of KAP to one another and the respondents’ profiles were analyzed using Chi-square test with a level of significance of 0.05. Results: Among 285 respondents, 59.3% had poor knowledge, 35.8% had wrong practices, and 21.8% had negative attitude on rabies prevention and control. We found that 92.6% did not know that rabies is incurable. As to practices, performing “tandok” (42.5%), or the act of removal of rabies from a wound by using an animal horn, and applying herbal medicines (34%) were still done. Use of dog restraint (44.6%) and euthanasia (40%) weren’t favorable to study participants. Surprisingly, non-dog owners had good knowledge and positive attitude than dog owners. Conclusion Our study showed that majority of the study population had poor knowledge on rabies prevention and control, on disease transmission, and on the incurability of rabies. While majority had a positive attitude and correct practices, the unacceptability on the use of a dog restraint and euthanasia, especially among dog owners, were still evident.
Rabies ; Knowledge

Rabies virus ; physiology

Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines ; Cells, Laboratories

Vnukovo-32 (cell culture rabies virus vaccine strain) was propagated experimentally in primary hamster kidney cell (PHKC), primary Chick embryo fibroblast cell (CEF) and human diploid cell (WI-38). These 3 cell lines were infected with Vnukovo-32 by the same mount of infection (MOl) - lLD50/l000 cells. The result showed that the Vnukovo-32 was adapted on all experimental cell lines, met the criteria of master seed (the titer ranging 6.4-7.5 log LD50/ml), and reached the highest titer on the 7th - 9th day postinfection in three cell groups. Moreover, the highest titer of Vnukovo-32 was met in human diploid cell (WI-38), then in PHCK, and the lowest was in CEF. From these results, it is suitable to use WI-38 to propagate Vnukovo-32 for master and working seed. The procedure for propagation of Vnukovo-32 is set up.
Rabies Vaccines ; Cells

Standard lyophylized rabid vaccine produced from CVS strain of Biopharma-Indonesia has met the criterion in terms of sterility, safety, efficacy (14.24 IU/ml), thermo-stability 37oC/month (5.4 IU/ml) and residual humidity (3.34%). Immune responsivity of the standard sample vaccine was determined by neutralizing method reaching 1/5 = 3.8 IU/ml; 1/25 = 1.7 IU/ml and 1/125 = 0.7 IU/ml
Rabies Vaccines ; Vaccines ; immunization

A purified rabie vaccine was prepared from mouse brain of CVS/P II from the Institute Bipharma-Indonesia. After refrigerating centrifuged, the vaccine was more pure and more efficacy. The sterility, innocuity and potency were perfect
Rabies Vaccines ; Vaccines ; immunization