The purpose of this study was to determine whether accumulated and temporary fatigue could be separately observed in repeated cycling sprints (RCS) with combined short and long recovery periods. Eight male performed three RCS with 35-sec (RCS₃₅), 350-sec (RCS₃₅₀) and combined 35-sec and 350-sec recovery periods (RCS_comb). RCS_comb consisted of ten 10-sec cycling sprints (CSs) with 35-sec and 350-sec recovery periods before the 5^th and 9^th CS. In RCS_comb, peak power output (PPO) was restored in the 5^th and not in 9^th CS. Blood lactate concentration ([La]) progressively increased, but there were no significant differences among conditions despite the difference in PPO. In RCS_comb, mean power frequency determined on the vastus lateralis was correlated with PPO and oxygen uptake before CSs (preVO₂) showed high in short recovery periods and low values in long recovery periods. Accumulated and temporary fatigue cannot be explained by effects of preVO₂ and [La].