1.Synthesis of a full-length coxsackievirus A9 RNA by in vitro transcription.
Journal of the Korean Society of Virology 1991;21(2):105-111
No abstract available.
RNA*
2.Quantitative measurement of surfactant protein B mRNA by filter hybridization.
Sung Soo PARK ; Dong Hoo LEE ; Dong Ho SHIN ; Jung Hee LEE
Tuberculosis and Respiratory Diseases 1992;39(3):242-247
No abstract available.
RNA, Messenger*
3.Sequencing analysis of 18S ribosomal RNA of human giant intestinal fluke (Fasciolopsis rusk/) collected in Ninh Binh and Nghe An provinces
Journal of Malaria and parasite diseases Control 2003;0(4):94-99
Fasciolopsis buski is common parasite in pigs. Some patients who infected by adult worms of Fasciolopsis buski in several areas, including the provinces of Ninh Binh and Nghe An were sampled. Genome of 18S ribosomal RNA with 1950 nucleotides from giant intestinal fluke (Fasciolopsis buski) was obtained using polymerase chain reaction (PCR) and sequencing approach for comparative analysis each others and with the data deposited in GenBank (L06668). The adult worms from Ninh Binh and Nghe An were morphologically identified as Fasciolopsis buski. The genetic analysis revealed that the 18S rRNA gene of the giant intestinal flukes isolated in Ninh Binh and Nghe An were similar (homogenous 100% of nucleotides) and showed nearly absolute identity with only 2 divergent nucleotides of 0.01% (2/1950 nucleotides) to the 18S rRNA of the pig Fasciolopsis buski as the data deposited in GenBank (L066608). However, 18S ribosomal RNA of the adult worms of human Fasciolopsis buski collected from the provinces of Ninh Binh and Nghe An were similar to one another
Fasciolidae
;
RNA
4.RNAi: A Recent Revolutionary Tool for Understanding of Basic Biology.
Dong Kil JUNG ; Young Suk CHO ; Won Kil LEE
Korean Journal of Clinical Microbiology 2007;10(1):1-5
RNA interference (RNAi) is a gene-silencing technology by which small double-stranded RNAs are used to target the degradation of RNA with complementary sequence. RNAi is found in a wide variety of organisms (Caenorhabditis elegans, insects, plants, microorganisms and animals). With RNAi, we have harnessed the gene function to be explored, revolutionized our ability to perform large-scale genetic screens, and even therapeutic potential.
Biology*
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Insects
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RNA
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RNA Interference
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RNA, Double-Stranded
5.RNA Regulation in Neurologic Disease and Cancer.
Cancer Research and Treatment 2010;42(3):125-129
The paraneoplastic neurologic diseases (PNDs) are brain degenerations that develop in the setting of clinically inapparent cancers. PNDs arise when common cancers express brain proteins, triggering an anti-tumor immune response and tumor immunity. Research on these brain-cancer proteins has revealed a new world of neuron-specific RNA binding proteins whose functions may be aberrantly used by tumor cells. Efforts to gain insight into their function has led to the development of new methods and strategies to understand RNA protein regulation in living tissues.
Brain
;
Proteins
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RNA
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RNA-Binding Proteins
6.The S-genomic RNA of Hantaan virus contains only two trinucleotide repeats at its 5' end and the 3' end of 3'-specific mRNA is truncated and lacks poly-A tail.
Michael DOBBS ; Mi Rim JIN ; C Yong KANG
Experimental & Molecular Medicine 1997;29(1):81-83
No abstract available.
Hantaan virus*
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RNA*
;
RNA, Messenger*
;
Trinucleotide Repeats*
7.Research Progress of circRNA and Its Significance in Forensic Science.
Ya-qi ZHANG ; Cheng-chen SHAO ; Cheng-tao LI ; Zi-qin ZHAO ; Jian-hui XIE
Journal of Forensic Medicine 2016;32(2):131-133
RNA has received more attention in the field of forensic medicine and the development of the new biological markers based on RNA shows great significance in the analysis of complex cases. circular RNA (circRNA) is a kind of non-coding RNA which is widely reported recently. Although the regulatory mechanisms of generation and expression are not fully clear, the existing research indicates that circRNA has important biological functions. CircRNA has a cell-type-specific expression with great stability and a high expression level, which makes it meaningful in forensic applications potentially. In this paper, the research progress, the generation and regulation of circRNA as well as its biological characteristics and functions are summarized, which will provide references for related studies and forensic applications.
Forensic Sciences
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Humans
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RNA
;
RNA, Circular
8.Safety and feasibility of PVA formaldehyde absorbent sponge in noninvasive uterine fluid sampling and RNA sequencing.
Xi HUANG ; Yanping LI ; Liya LI ; Aihua HE
Journal of Central South University(Medical Sciences) 2022;47(11):1504-1511
OBJECTIVES:
Uterine fluid RNA can be used as a test for endometrial receptivity, but there is still no noninvasive sampling method available. The polyvinyl alcohol (PVA) formaldehyde absorbent sponge, a medical bio-absorbent sponge with good water absorption and biophilic properties, can be used to develop a new noninvasive endometrial fluid sampler. This study aims to investigate the toxicity of PVA acetal absorbent sponges on endometrial epithelial cells and its effect on RNA sequencing (RNA-Seq).
METHODS:
The experimental group using PVA formaldehyde absorbent sponge was prepared into 0.005%, 0.01% and 0.02% (w/v) suspension, and 0.01%, 0.05% and 0.1% (v/v) extract groups. The control group was only the complete culture medium. Nothing was added to the blank group. In vitro cytotoxicity assay was used to evaluate the survival rate of cells. Eight patients underwent in vitro fertilization treatment in the Reproductive Center of Xiangya Hospital, Central South University from November 2019 to January 2020. The uterine fluid of each patient was aspirated. The experimental group was inhaled with sterile PVA formaldehyde absorbent sponge and then immersed RNA-later solution. The control group was directly injected into the same amount of RNA-later solution. RNA-seq and data analysis was performed later.
RESULTS:
The vitro cytotoxicity assay showed that in suspension groups, there was no significance difference in cell survival between different co-culture time in 0.005% group (P=0.255). In the 0.01% and 0.02% group, there was no difference at each incubation time within 12 h (all P>0.05), but the cell survival rate was decreased at 24 h compared with 0 h (P<0.01, P<0.05). At the same co-culture time, the cell survival of the 3 concentration gradient groups were significantly lower than that of the control group (all P<0.05). The cell viability of the 0.005% concentration group was decreased less than 30% at 24 h, the 0.01% concentration group decreased more than 30% at 12 h, and the 0.02% concentration group was decreased more than 30% at 0 h. For extract groups, there was no significant difference in the survival rate within 6 h in 0.01% concentration group (all P>0.05), and the survival rate of 12 h and 24 h was lower than that of 0 h group (both P<0.01). In 0.05% group, there was no significant difference at each incubation time within 12 h (all P>0.05), but the survival rate at 24 h was lower than that at 0 h (P<0.05). There was no significant difference in survival rate at different culture time in 0.1% concentration group (P=0.082). At the same culture time, there was no significant difference in survival rate between 0.01% group and control group at 0, 3 and 24 h (all P>0.05). Except for 3 h, the survival rate of 0.05% and 0.1% groups was lower than that of control group (all P<0.05), and the decrease was all less than 30%. Uterine fluid RNA-seq showed that there was no significance difference in exonic rate, the detected genes and transcripts of RNA between the experiment groups and the control group (all P>0.05).
CONCLUSIONS
The in vitro cytotoxic of PVA formaldehyde absorbent sponge on human endometrial epithelial cell meet the national standard of the cytotoxic of medical materials. Sampling the uterine fluid with this material does not affect the RNA-Seq results. PVA formaldehyde absorbent sponge is safe and feasible when appling to the noninvasive uterine fluid sampling and RNA sequencing.
Humans
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Social Group
;
Sequence Analysis, RNA
;
RNA
9.Circular RNAs: typical biomarkers for bone-related diseases.
Linghui HU ; Wei WU ; Jun ZOU
Journal of Zhejiang University. Science. B 2022;23(12):975-988
Bone is a connective tissue that has important functions in the human body. Cells and the extracellular matrix (ECM) are key components of bone and are closely related to bone-related diseases. However, the outcomes of conventional treatments for bone-related diseases are not promising, and hence it is necessary to elucidate the exact regulatory mechanisms of bone-related diseases and identify novel biomarkers for diagnosis and therapy. Circular RNAs (circRNAs) are single-stranded RNAs that form closed circular structures without a 5' cap or 3' tail and polycyclic adenylate tails. Due to their high stability, circRNAs have the potential to be typical biomarkers. Accumulating evidence suggests that circRNAs are involved in bone-related diseases, including osteoarthritis, osteoporosis, osteosarcoma, multiple myeloma, intervertebral disc degeneration, and rheumatoid arthritis. Herein, we summarize the recent research progress on the characteristics and functions of circRNAs, and highlight the regulatory mechanism of circRNAs in bone-related diseases.
Humans
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RNA, Circular
;
RNA
;
Biomarkers
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Osteoarthritis
10.Induction of IL-lα mRNA Expression by 17β-Estradiol in Normal Human Keratinocytes.
Chuluundorj DELGERTSETSEG ; Seok Beom HONG ; Young Il KIM ; Nack In KIM
Annals of Dermatology 2004;16(4):163-168
No abstract available.
Humans*
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Keratinocytes*
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RNA, Messenger*