Cell Adhesion ; drug effects ; Cytokines ; blood ; drug effects ; Humans ; Paraquat ; toxicity

Esophageal cancer is one of the most common malignant tumors in China.At present, the best treatment of esophageal cancer is surgery.For the patients with cervical lymph nodes metastases, three-field lymph nodes dissection is the main solution.Ultrasonography and computed tomography (CT) of the neck play a decisive role in the preoperative examinations for patients with esophageal cancer, and should be used as routine examinations before surgery at the qualified hospital in order to improve the accuracy of preoperative assessment of cervical lymph nodes metastases of esophageal cancer and screen potential patients with esophageal cancer undergoing three-field lymph nodes dissection.

Objective To investigate the safety and clinical effect of Ⅰ-stage resection of colon cancer and synchronous liver metastasis after conversion therapy.Methods The retrospective descriptive study was adopted.The clinical data of 1 patient with sigmoid cancer with synchronous multiple liver metastases who was admitted to the Zhongshan Hospital of Fudan University in April 2013 were collected.The patient was diagnosed as sigmoid cancer with multiple lymph nodes surrounding colon and 5 metastatic lesions found in liver by preoperative imaging examination,and the maximum diameters of 2 metastatic lesions were 4.5 cm and 3.6 cm.The pathological results of colonoscopy indicated adenocarcinoma.After discussion of the multidisciplinary team,liver metastatic lesions were significantly reduced through mFOLFOX6 chemotherapy combined with bevacizumab treatment,and then patient underwent Ⅰ-stage resection of colon cancer with synchronous liver metastasis by the surgical procedures of partial hepatectomy + 3D laparoscopy-assisted radical resection of sigmoid cancer.The patient received convention treatment of antibiotic,nutrition support therapy and mFOLFOX6 adjuvant chemotherapy.The changes of lesions in liver and tumor markers were observed by tumor marker test,abdominal/pelvic CT and MRI in the upper abdomen after conversion therapy.The operation time,volume of intraoptrative blood loss,number of lymph nodes dissected and vital sign were observed in the operation.The liver function,time for out-off-bed activity,time to anal exsufflation,time of drainage tube removal,duration of hospital stay,complications,results of pathological examination and recurrence and mnetastasis of tumor were observed after operation.After discharge from hospital,the patient underwent monthly tumor marker test,B ultrasound and chest X-ray examination till January 2014 and abdominaL/pelvic CT,magnetic resonance imaging (MRI) in the upper abdomen and colonoscopy every 6 months.The follow-up of outpatient examination and telephone interview was performed to detect the recurrence and metastasis of tumor up to September 2015.Results After preoperative 4-cycle chemotherapy,the diameters of 2 metastatic lesions in liver reduced to 3.2 cm and 2.0 cm,the levels of carcinoembryonic antigen(CEA) and CA19-9 (tumor marker) were reduced to 95.9 μg/L and 252.4 kU/L.The patient underwent successful Ⅰ-stage resection of colon cancer and synohronous liver metastasis.The operation time,volume of intraoperative blood loss,numbers of lymph nodes dissected and cancerous nodes were 208 minutes,250mL,14 and 1,respectively.The patient had no blood transfusion and metastasis,with the stable vital signs and good postoperative recovery.The levels of alanine transaminase (ALT) and aspartate transaminase (AST) were 1 147 U/L and 2 631 U/L at postoperative day 1,101 U/L and 37 U/L at postoperative day 7,respectively.The out-off-bed activity,anal exsufflation,drainage tube removal and discharge from hospital were occurred at postoperative day 2,3,7 and 9,respectively.No anastomotic fistula,bleeding and infection were occurred aftcr operation.The patient was diagnosed as with ulcerated sigmoid adenocarcinoma in T3N1cM1 stage (combining with liver metastasis).The patient received adjuvant therapy of mFOLFOX6 and oral capecitabine at postoperative day 40,and was followed up for 30 months with a good quality of life and without metastasis and recurrence.Conclusion Ⅰ-stage resection of colon cancer with synohronous liver metastasis can be used for initial unresectable colon cancer with liver metastasis after conversion therapy,and it is safe and feasible,with a good therapeutic effect.

Analysised the transmission routes of AIDS in hospital,preventive strategies and existing ethic problems.Putting forward the importance of respecting the proper right of AIDS patients and health care workers in the prevention of nosocomial-transmitted HIV.On the basis of scientific study,strengthening the propagating of the knowledge of AIDS in the whole society,respecting the right and statue of AIDS prevention and timely treatment among both patients and health care workers.Respecting their medical treatment right and secrets,detecting HIV of patients on the informed consent.Respecting health care workers' right,providing profession guide and preventive strategies for prevention and control of nosocomial-transmitted AIDS.

OBJECTIVE:To study the characteristics and general pattern of the adverse drug reactions(ADR)occurred in our hospital.METHODS:A total of 125 ADR cases collected in our hospital during 2007 were analyzed statistically in respect of patients' age and sex,route of administration,serious ADR,drug combination,past ADR history,drug kinds,ADR-involved organs or systems and clinical manifestations etc.RESULTS:Of the total 125 ADR cases,11 categories or 62 kinds of drugs were involved,with antimicrobial drugs took the lead which caused ADRs in 65 cases(52.0%)followed by Chinese patent agents which caused ADR in 38 cases(30.4%).102(81.6%)ADR cases were induced by intravenous route.Lesions of skin and appendants were the most common presentations of the ADR,which appeared in 70 cases(52.24%).10(8.0%)were severe cases.CONCLUSION:Importance should be attached to the reporting and monitoring of ADR so as to lessen or avoid the occurrence of ADR in the clinic meanwhile promoting rational use of drugs and ensure medication safety.

Objective To investigate the correlationbetween thecarcinoembryonic antigen ( CEA) level and hypoxic status of breast cancer, and itspotential of being a prognostic factor in breast cancer. Methods Breast cancer cell line MDA-MB-231 was cultured under hypoxic or normoxic condition to determine the protein level of CEA using Western blotting.30 breast cancer tissue sections were collected in Tianjin Medical University Cancer Institute and Hospital from April 2013 to June 2015.and were detected the expression of CEA, hypoxia-inducible factor-1α( HIF-1α) and carbonic anhydrase-9 ( CA-9 ) by immunohistochemistry.Serum CEA were detected by immunofluorescence assay of 166 breast cancer patients collected from April 2009 to June 2011.The correlation between the expression levels of CEA and breast cancer patients'clinical data was analyzed by Logistic univariate analysis.Kaplan-Meier method was employed to calculate the progression-free survival of patients with breast cancer. Log-rank test was conducted to compare the difference between groups.Results CEA levels were significantly higher in breast cancer cells under hypoxic condition in a time-dependent manner, compared to normoxiccontrol. Immunohistochemical staining indicated that CEA was coexpressed with HIF-1αand CA-9 in breast cancer and linear regression analysis revealed that CEA expression correlated with HIF-1α( P =0.0096 );Preoperative serum levels of CEA were closely related with tumor size ( P =0.015 ) andLymph node metastasis(P=0.032); CEA positive patients achieved a progression-free survival of 29.85months(versus 39.08 months in CEA negative patients, P=0.003).Conclusions Hypoxia upregulates CEA expression in breast cancer and the levels of preoperative serum CEA is a potential prognostic factor ofbreast cancer.

Objective:To provide ideas for the participation of clinical pharmacists in clinical individualized medication.Methods:Clinical pharmacists participated in the clinical consultation for one senior patient with acute cholangitis treated with biapenem.Results:The consultation comments and suggestions proposed by clinical pharmacists were accepted by clinics,which played an important role in assisting doctors in the rational drug use and significantly improved the medical treatment.Conclusion:Clinical pharmacists should participate in individualized medication and help clinicians optimize drug therapy,which can improve the safety and efficacy of medication.

Long Jing 1 (L01) is the effective component extracted from asiatic todd-alia. In the experiments on rat thoracic aortic rings, L01 45-405 ?mol/L inhibited thecontraction initiated by high K~+ or NE, the IC_(50) value was 206.93 and 94.18 ?mol/Lrespectively. L01 shifted the dose-response curve of KCl or NE to the right, and reducedthe maximal response, also shifted that of CaCl_2 to the right parallelly. The effects ofL01 were similar to that of Ver (verapamil) in the blockage on PDC. Morever L01 80 ?mol/Linhibited the release of intracellular Ca~(2+) and extracellular Ca~(2+) influx initiated by NE,the former effect was more significant than the latter (this was different from Ver). Theseresults suggested that L01 was probably a new calcium antagonist different from Ver.

Objective To investigate the efficacy of low dose thalidomide combined with chemotherapy in treatment of multiple myeloma(MM)and the clinical curative effect of the treatment method.Methods From June 2010 to June 2012,80 patients were diagnosed with multiple myeloma as the research object in Traditional Chinese Medicine Hospital of Haining,randomly divided into control group and observation group,with 40 cases in each group.The control group adopted conventional chemotherapy,the observation group were given low dose thalidomide combined with conventional chemotherapy.The clinical effect and adverse drug reactions were compared. Results The total effective rate of observation group (87.5%)was significantly higher than that of control group(65.0%)(P<0.05).The adverse reactions were 87.5%(35/40)in observation group and 100.0%(40/40)in control group,there was no significant difference between the two groups.The mean progression-free survival(PFS)and overall survival(OS)in observation group(5.5 ±1.2 months;1 1.5 ±2.4 months)were higher than those in control group(3.7 ±0.8 months;8.5 ±1.3 months)(P<0.05 ). Conclusion Low dose thalidomide combined with conventional chemotherapy in the treatment of multiple myeloma can improve clinical effect and decrease recurrence rate,with high safety and less adverse reactions.

BACKGROUND:Although cementation of polyethylene liner into a wel-fixed metal shel during the revision surgery has several advantages compared to total acetabular revision, there are some disputes about its clinical outcomes, especialy regarding the re-revision rate and the prosthetic survivorship. OBJECTIVE:To evaluate the clinical outcomes and safety of cementation of polyethylene liner into a wel-fixed acetabular cup in the revision after total hip arthroplasty with retained stable cup. METHODS:Between March 2012 and November 2014, nine patients underwent revision after total hip arthroplasty with cementation of polyethylene liner into a wel-fixed acetabular cup. There were 1 male and 8 females, aged 43.7 years on average. The time was 9.4 years on average from the primary total hip arthroplasty to revision. Harris Hip Score and prosthesis X-ray films were compared after revision. The effects and safety of cementation of polyethylene liner into a wel-fixed acetabular cup were further evaluated. RESULTS AND CONCLUSION:Nine patients were folowed up for 8-37 months. The Harris hip scores before revision was (49.54±18.28) points previously, and (84.82±10.59) points during final folow-up (t=6.377, P < 0.05). One patient had re-revision surgery because of recurrent dislocation. There were not complications in other patients, resulting in failure rate of 11%. These results indicated that polyethylene liner cementation technique is safe and effective when the wel-positioned and wel-fixed acetabular cup retained.