143 bacterial strains isolated in Vietnam for the cephalosporin C acylase activity. Among them, 116 strains showed growth on the LB plates containing cephalosporin and 28 strains did not grow. From 7 strains which grew very strongly on the LB plates containing cephalosporin C, 6 strains designated as 71, 91, DT3, M6, 03 and Tb23 were selected for further cultivation in the liquid culture medium and for determination of CA activity. The cell growth curves of these strain were very similar. The cell density reached a maximum at 24-30 hours of cultivation. Among three strains 71, DT3 and Tb23 evaluated for the CA activity, DT3 showed the highest CA activity. Thus, the strain DT3 was identified as Serratia marcescens sp. DT3. This is the first report about the Serratia strain producing CA enzyme
Pharmaceutical Preparations ; pharmacy ; chemistry