1.The detection on the p24 gene fragment of Borna disease virus in brain tissues of animals in Ili, Xin-jiang
Feng HE ; Yuxing FENG ; Houchao SUN ; Zicheng HU ; Hongbo XU ; Mingming XU ; Qunling ZHAN ; Yongbo HU ; Ge JIN ; Yingying ZHANG ; Leilei LI ; Peng XIE
Chinese Journal of Microbiology and Immunology 2010;30(1):31-35
Objective To observe the epidemiology characterization of Borna disease virus (BDV) in animal brain in Ili, Xinjiang, and to find out the potential infection of the Borna disease virus to prevent its outbreak. Methods The BDV p24 gene of animal brain tissues in Ili including 200 horses, 75 donkeys and 100 shepherd dogs was detected by fluorescence quantitative nest reverse transcriptase polymer-ase chain reaction(FQ-nRT-PCR). GFP-p24,pMD-19 plasmid contamination was excluded from positive products. Clone sequencing was used to analyze the homology of gene and amino acid sequence. Results BDV p24 gent was found in 3 Ili horses, 4 Ili donkeys and 9 shepherd dogs, and the positive ratio is 1.5%, 5.3% and 9.0%, respectively. The GFP-p24,pMD-19 were not found in BDV p40 gene and plasmid stand-ard. The sequence of BDV p24 amplification production was totally the same as He/80 virus strain. Conclu-sion Natural infection of BDV may exist in the animals(horses, donkeys and dogs)in Ili, and the epidem-ic strain of BDV in this area was homological as He/80 virus strain.
2.Epidemiological investigation of Borna disease virus infection in horses and donkeys in Yili, Xinjiang
Yiagying ZHANG ; Qunling ZHAN ; Mingming XU ; Jianping YU ; Zhilei ZENG ; Hong ZHA ; Yanxi LIU ; Xiao CHEN ; Dan PENG ; Dan ZHU ; Yongbo HU ; Kang HUO ; Peng XIE
Chinese Journal of Microbiology and Immunology 2009;29(4):321-325
Objective To investigate the epidemiology of BDV infection in Yili horses and Yili donkeys and to analyze phylogenetic source of BDV in Yili area, Xinjiang. Methods We established fluo- rescence quantitative nested RT-PCR to detect BDV p24 segment in peripheral blood mononuclear cells (PBMCs) of 518 Yili horses and 206 Yili donkeys in Yili area, Xinjiang. Positive products were validated by detecting BDV p40 segment and plasmid to preclude the contamination, and were sequenced to analyze the homology of gene sequence, amino acid sequence and phylogenetic tree. Results The positive rates of BDV infection in PBMCs of 518 Yili horses and 206 Yili donkeys were 0.97% and 1.94%, respectively. The results of BDV p40 segment verification were positive in all of the samples of BDV p24 positive. All the samples tested were not contaminated by plasmid. There was a homology of the gene sequence of positive PCR samples with strain He/80. And the gene sequence revealed more than 93% identical to H1766 and strain V. Conclusion Our study suggested BDV natural infection in Yili horses and Yili donkeys. The en- demic BDV had a high degree of identity to strain He/80.
3.Efficacy and safety of high-frequency repetitive transcranial magnetic stimulation for post-stroke depression :A systematic review
Liang CHEN ; Jie CHEN ; Ge JIN ; Ling HU ; Xinggui LI ; Qunling ZHAN
Chinese Journal of Physical Medicine and Rehabilitation 2018;40(6):441-448
Objective To evaluate the efficacy and safety of high-frequency repetitive transcranial magnetic stimulation (rTMS) in the treatment of post-stroke depression (PSiD).Methods The Cochrane Library,Medline,EMbase and CBMdisc were electronically searched for reports of randomized and controlled trials (RCTs) of high-frequency rTMS in the treatment of PSD before October 2017.Two researchers screened the reports independently,extracted data and assessed the risk of bias in the included studies.The meta-analysis was performed using version 5.1 of the RevMan software.Results A total of 26 RCTs were included,covering 1810 patients with PSD.The metaanalysis showed that the remission rate [relative risk (RR) =2.15,95%CI (1.64,2.83)] and the effectiveness [RR =1.66,95%CI (1.28,2.15)] of high-frequency rTMS group were significantly higher than in the control groups.Compared with the control group,the average Hamilton depression rating scale (HAMD) score of the high-frequency rTMS groups was significantly lower than in the control groups.There was no significant difference in withdrawal between the two groups after treatment [RR =0.55,95%CI (0.18,1.65)].The incidence of headaches was significantly higher in the high-frequency rTMS group than among the controls.Conclusion High-frequency rTMS has a good curative effect and better acceptability for PSD patients,but attention should be given to headaches and other adverse reactions.Due to the limited number of studies and their quality,these conclusions still need to be verified in betterquality studies.
4.Effect of long cotton branch instead of cotton ball in postoperative oral nursing of patients in the department of neurosurgery
Qunling HU ; Jufang ZHANG ; Yuying WENG ; Xiamin QIU
China Modern Doctor 2015;(12):134-136
Objective To investigate the effect of long cotton branch instead of cotton ball in postoperative oral nursing of patients in the department of neurosurgery. Methods All 72 cases of coma from January 2013 to January 2014 were used long cotton branches in postoperative oral nursing (observation group),72 cases of coma from December 2011 to December 2011 were used cotton ball in postoperative oral nursing (control group), the effect of nursing care, oral cavity cleaning degree,nurse fatigue degree score and complication were compared. Results The nursing efficient,oral cavity cleaning degree and nurse fatigue degree score of the observation group was significantly better than the control group(P<0.05);the complication rates of the observation group was significantly lower than the control group(P<0.05). Conclusion Use long cotton branch instead of cotton ball in postoperative oral nursing of patients in the department of neurosurgery,could get more significant clinical effect and lower complication rates.