Objective To investigate the distribution characteristics and antimicrobial resistance of carbapenem-resistantAcinetobacter baumannii (CRAB),and to guide effective clinical prevention and rational antimicrobial use. Methods Data about clinically isolated CRAB between January 2009 and December 2013 were analyzed retrospec-tively,distribution and antimicrobial resistance were analyzed by WHONET 5.5 software.Results A total of 888 Acinetobacter baumannii strains were isolated,421 of which were CRAB,the isolation rate was 47.4%,the isola-tion rates in 2011 ,2012 and 2013 were all about 50.0%;CRAB strains were mainly isolated from sputum (73.4%) and mostly distributed in intensive care unit (ICU)(61 .3%),followed by neurosurgery department (12.4% ). CRAB presented highly antimicrobial resistance.Except cefotaxime and cefatriaxone,resistant rates of CRAB to the other detected antimicrobial agents(ceftazidime,cefepime,cefoperazone/sulbactam,aztreonam,imipenem,amika-cin,gentamycin,minocycline,chloramphenicol,levofloxacin,ciprofloxacin,and compound sulfamethoxazole)were all higher than non-CRAB isolates(all P ≤0.01),Compared with non-CRAB isolates,The resistant rate of CRAB to cefoperazone/sulbactam was the lowest(<15%),followed by minocycline,resistant rates to other antimicrobial agents were all >80.0%.Conclusion Surveillance of CRAB should be further strengthened.It is necessary to fo-cus on the control and prevention of healthcare-associated infection in ICU patients and respiratory system.

OBJECTIVE To explore the distribution of 16S rRNA methylase and aminoglycoside modifying enzymes in continuous isolates of Pseudomonas aeruginosa(PAE).METHODS The antibiotics susceptibility was tested by K-B method,the 16S rRNA methylase(rmtB) and aminoglycoside modifying enzymes were detected by polymerase chain reaction(PCR),the positive genes were amplified and sequenced by PCR fluorescence spectrophotometry.RESULTS Among 20 strains of PAE,the 16S rRNA methylase and 5 kinds of aminoglycoside modifying enzymes had been detected.The 5 kinds of aminoglycoside modifying enzymes were aac(3)-Ⅱ,aac(6′)-Ⅰb,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ,respectively.And more,the rmtB in the first and third strains was sequenced,and translated into amino acid,and the translated amino acid sequence was compared with GenBank,suggesting there be different amino acid sequence.This was confirmed as two new subtypes.CONCLUSIONS Resistant to antibiotics PAE in our hospital is mainly related to 16S rRNA methylase and 5 aminoglycoside modifying enzymes,and 2 new subtypes of 16S rRNA methylase are discovered.

Objective To study the correlation between MGMT expression , clinicopathologic features and post-chemotherapy prognosis in patients with primary central nervous system lymphoma diffuse large B-cell lymphoma (PCNS-DLBCL).Methods MGMT expression was detected in 76 cases of PCNS-DLBCL by EnVision method with immunohistochemical staining .Follow-up data including treatment response and overall survival time , were analyzed.Results The rate of MGMT expression in PCNS-DLBCL was 67.1%(51/76).The MGMT expression rate in male patients was higher than that in female (P<0.05). Univariate analysis showed that these clinical pathological characteristics affected the overall survival of PCNS-DLBCL patients , including age and Hans algorithm , although no statistical significance was detected ( P value was 0.065 and 0.069 respectively ) .The overall survival of the patients with positive MGMT and aged over 60 years was shorter after chemotherapy than those without chemotherapy ( P=0.022 ) .In the patients aged over 60 years, the prognosis of MGMT-positive patients was significantly better than MGMT-negative patients ( P=0.044 ) .Conclusions The expression of MGMT is more commonly found in male patients.In the patients aged over 60 years with the same therapy , the prognosis is better in the MGMT-negative ones.Detection of MGMT protein expression can provide some guidance in choice of treatment modalities in PCNS-DLBCL patients.