Abstract: Objective　To explore the tolerance of Aedes albopictus to typical insecticides and mutations in its knockdown resistance (kdr) and acetylcholinesterase number one (ace-1) genes in Hainan Province. Methods　Ae. albopictus larvae were collected from Danzhou, Sanya, Qiongzhong, Qionghai, Changjiang, and Wanning in Hainan Province in 2020 and 2021 to assess their resistance to 10 insecticides. Surviving samples from the resistance tests were examined for mutations in genes encoding acetylcholinesterase (AChE) and voltage-gated sodium channel (VGSC) using DNA Sanger sequencing. Results　The 24-hour corrected mortality rates of adult Ae. albopictus in different areas of Hainan to deltamethrin (0.03%), permethrin (0.40%), Beta-cypermethrin (0.40%), Beta-cypermethrin (0.08%), and lambda-cyhalothrin (0.07%) ranged from 5.56% to 83.33%, 29.89% to 92.22%, 26.67% to 97.78%, 11.11% to 88.89%, and 40.00% to 83.33%, respectively. The mortality rates after 24-hour exposure to propoxur (0.05%), bendiocarb (0.20%), malathion (0.50%), fenitrothion (0.20%), and chlorpyrifos (2.00%) ranged from 94.89% to 100.00%, among which the Ae.albopictus population from Changjiang showed a mortality rate of 97.78% to propoxur, and populations from Sanya, Wanning, and Qionghai showed mortality rates of 94.89%, 96.67%, and 96.67% to malathion respectively. The mortality rate of Ae.albopictus populations in Sanya and Qionghai to fenitrothion was 97.78%, while in other populations, it was 100.00%. Sequencing results showed that the kdr gene exhibited a mutation solely at locus 1534, featuring four alleles: wild-type TTC/F and the mutant types TGT(TGC)/C, TCC/S, and TTG/L with the mutation frequencies of 40.47%, 22.25%, 36.44%, and 0.85%, respectively. There are seven types of these mutations: wild-type homozygotes F/F, wild-type/mutant heterozygotes F/C, F/S, and F/L, mutant heterozygotes S/C, and wild-type homozygotes C/C and S/S, with mutation frequencies of 19.92%, 6.78%, 32.63%, 1.69%, 11.44%, 13.14%, and 14.41%, respectively. Notably, No related amino acid mutations were detected at position 119 of the ace-1 gene. Conclusions　The Ae.albopictus populations in various areas of Hainan Province have developed a high resistance to pyrethroid insecticides such as deltamethrin, permethrin, beta-cypermethrin, and beta-cyfluthrin, but are sensitive or possibly resistant to carbamates and organophosphates insecticides. The resistance gene mutations mainly occur at position 1 534 of the kdr gene, suggesting that its mutation frequency could be a significant factor contributing to resistance in Ae. albopictus in Hainan.

Abstract: Objective　To understand the level of Aedes aegypti resistance in Hainan and to provide a reference for developing local preventive and control measures against the dengue vector Ae. aegypti. Methods In 2020, Aedes aegypti larvae and pupae were collected from Danzhou and Changjiang in Hainan Province and reared to the first filial generation (F1). The adult mosquito contact tube method was used to determine the resistance of Aedes aegypti to commonly used insecticides. The allele-specific PCR (AS-PCR) was used to detect the mutations in V1016G and F1534C knockdown resistance (kdr) gene in Aedes aegypti. Results There was no significant difference in the knockdown rate of Ae. aegypti from Changjiang and Danzhou after 1 hour of exposure to four pyrethroids insecticides, namely deltamethrin (0.03%), permethrin (0.40%), beta-cypermethrin (0.04%) and lambda-cyhalothrin (0.02%). However, the 24-hour mortality rates of Ae. aegypti from Changjiang and Danzhou city were 95.56%, 85.56%, 81.11%, 48.89% and 94.44%, 68.60%, 61.11%, 30.00%, respectively. Except for deltamethrin (0.03%), the other differences were statistically significant. Aedes aegypti in Changjiang showed 100.00% mortality to propoxur (0.03%) and bendiocarb (0.20%), while in Danzhou, these rates were 100.00% and 98.89%, respectively. Ae. aegypti both from Changjiang and Danzhou showed 100.00% mortality to malathion (1.50%) and chlorpyrifos (0.80%), and mortality rates to 0.25% fenthion were 100.00% and 98.89%, respectively. V1016G and F1534C mutations were found in Ae. aegypti from Danzhou and Changjiang with mutation frequencies of 12.50% (20/160) and 97.50% (156/160), showing significant differences (χ2=233.54, P<0.001). In Changjiang, Aedes aegypti exhibited both V1016G and F1534C mutations with frequencies of 20.00% and 100.00%, respectively, whereas in Danzhou, only F1534C mutations occurred with a frequency of 98.75%. Conclusions Ae. aegypti in Hainan Province exhibited varying levels of resistance to four insecticides: deltamethrin, permethrin, beta-cypermethrin, and lambda-cyhalothrin, but showed sensitivity to propoxur, bendiocarb, malathion, and chlorpyrifos. The primary knockdown resistance gene mutation was F1534C.

OBJECTIVETo explore the effects of warm needling combined with Zhangmo decoction (see text) on endometrial receptivity in patients with clomiphene (CC)-induced ovulation.

METHODSOne hundred and sixty cases were randomly divided into a CC group (group A), a CC+ progynova group (group B), a CC+ Zhangmo decoction group (group C) and a CC+ Zhangmo decoction + warm needling group (group D), 40 cases in each one. In the Group A, CC alone was applied. In the group B, progynova was jointly used on the 8th day of menstrual cycle. In the Group C, Zhangmo decoction was jointly used on the 5th day of menstrual cycle. In the group D, based on treatment of the Zhangmo decoction, warm needling was applied at Guanyuan (CV 4), Zhongji (CV 3) and Zigong (EX-CA 1) etc. The endometrial thickness and type, pulsatility index (PI), resistance index (RI), ratio of S/D on day of human chorionic gonadotropin (HCG) and pregnancy rate were observed in fou groups.

RESULTSThe PI, RI and S/D in the group C and D were obviously lower than those in group A and B (all P < 0.01). The endometrial thickness was (7.7 +/- 1.49) mm in group B, (8.2 +/- 1.54) mm in group C and (8.9 +/- 1.51) mm in group D, which were significantly different from (6.4 5 +/- 1.26) mm in the group A (all P < 0.01) also there was a significant difference between group C and D (P < 0.05). The rate of endometrial type A was 65.0% in the group D, which was significantly higer than 27.5% in the group A, 32.5% in the group B and 35.0% in the group C (all P < 0.01). The pregnancy rate was 30.0% in the group D, which was obviously higher than 12.5% in the group A, 15.0% in the group B and 17.5% in the group C (P < 0.05). The endometrial thickness and rate of endometrial type A in the pregnant were obviously higher than those in the non-pregnant (both P < 0.01) while PI, RI and S/D was lower than those in the non-pregnant (P < 0.01, P < 0.05).

CONCLUSIONWarm needing combined with Zhangmo decoction could improve endometrial thickness, morphology and uterine spiral artery to improve pregnancy rate, which has superior effect to clomiphene, clomiphene combined with progynova and clomiphene combined with Zhangmo decoction.

Chorionic Gonadotropin ; metabolism ; Clomiphene ; administration & dosage ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Embryo Transfer ; Female ; Fertilization in Vitro ; Humans ; Infertility, Female ; drug therapy ; metabolism ; physiopathology ; therapy ; Ovulation Induction ; Pregnancy

Ginsenosides,which belongs to triterpenoid saponins of plant terpenoids,are the main active components of the valuable medicinal herbs ginseng and American ginseng.Recent studies show that ginsenosides have a variety of beneficial effects,including anti-inflamatory,antioxidant,and anticancer effects.Ginsenosides are synthesized by complicated modification of triterpenoid skeleton after cyclization of 2,3-oxidosqualene through triterpene saponin synthesis pathway in which they share common early steps with phytosterols synthesis.It outlines recent advances for the biosynthesis of ginsenosides,the cloning and characterization of genes encoding key enzymes in the pathway and the basal framework of ginsenosides biosynthesis pathway.The prospects of secondary metabolism engineering in the biosynthesis of plant natural products and its application in ginsenosides biosynthesis are also discussed.

Objective To explore exenatide in the treatment of metformin(MET)alone,sulfonylurea (SU)alone or MET + SU combination therapy with poor glycemic control in type 2 diabetic pa-tients and to find effective nursing measures.Methods 24 patients were randomly divided into the con-trol group and the exenatide group with 12 patients in each group.In the exenatide group,exenatide 5μg twice a day for 4weeks,then 10μg twice a day for 12 weeks.Changes of HbAlc,body weight,BMI,FBG,P2hBG,and rate of adverse reaction were compared between two groups.Results Glycosylated hemoglobin (HbAlc),body weight,BMI,FBG,P2hBG in the control group before and after treatment showed no significant difference,while the exenatide group showed better results compared with those before treatment and the control group.Nursing intervention played evident effect on reducing adverse effect such as nausea,vomiting,diarrhea,low blood sugar,headache.Conclusions For patients with type 2 diabetes,using MET,SU alone or MET + SU combination therapy showed poor results of blood sugar control,addition of exenatide therapy can effectively control blood sugar,nursing intervention can significantly alleviate the adverse effects of patients.

To improve the antibiotics production of Streptomyces qinlingensis sp. nov.,protoplast regeneration combined with physical and chemical mutagenesis was used to selected high-yielding strains. The results showed that the antibacterial activities of strain R-72 from protoplast regeneration and NTG-1,H30-7 from protoplast mutagenesis against Bacillus subtilis were more than 20% higher than that of the original strain,and the heredity characters of those strains were stable in successive ten generations. The further bioassay experiments exhibited that the fungicidal and antibacterial activities of the fermentation broth from R-72,NTG-1 and H30-7 were remarkable increased comparing with that of the starting strain.

OBJECTIVE:To establish the system of curriculum based on working process and occupation ability for pharmacy major in higher vocational colleges. METHODS:Investigation was conducted among medical practitioners from pharmaceutical companies,hospitals,pharmaceutical factories,scientific research institutions and other related professionals. RESULTS:150 ques-tionnaires were sent out,and 141 valid questionnaires were collected with effective recovery rate of 94.00%. Results of investiga-tion showed that respondents most valued graduates with interpersonal and communication skills,followed by professional skills and practical ability. They were mainly clinical application of drugs,pharmacological effects and adverse reactions of drugs in the pharmacology theory teaching,the mechanism of action of drugs were weakened. The ability of prescription distribution,symptoms inquiring and drugs recommending should be strengthened in the pharmacology theory practice teaching. More than half of the re-spondents thought that confirmatory tests were necessary to keep,which helped to train students’practical ability and deepen the understanding of the theory. Meanwhile,it was important to strengthen the students’communication with the patients or their fami-lies and doctors to cultivate the ability of acquiring professional knowledge. CONCLUSIONS:The investigation provides basis for the making of curriculum standards of pharmacology,through which teaching contents are selected,teaching methods are de-signed,and it makes the pharmacology course full of post applicability and provides better decision-making basis to meet the posi-tion requirements.

Abstract: Objective　To understand the density changes and seasonal fluctuation of larvae and adults of Aedes albopictus in Hainan Province from 2017 to 2021, and to provide a scientific basis for the prevention and control of dengue fever and other vector-borne diseases in Hainan Province. Methods　The Breteau index method was used to monitor the larvae of Aedes mosquitoes, and the breeding sites of all small indoor and outdoor water containers and their larvae was checked and recorded. The adult mosquitoes were monitored by double lamination and light trap. Results　A total of 1 962 adult female Aedes albopictus mosquitoes were captured in Hainan Province from 2017 to 2021, accounting for 2.17% of the total captured mosquitoes. There were significant differences in mosquito species composition in different habitats in different years (χ2=312.258, P<0.01). In 2017, the density of mosquitoes in livestock sheds was the highest (9.71 per lamp·night), but the composition ratio of Aedes albopictus was the lowest (1.29%). In 2020, the mosquito density in hospitals was the lowest (2.22 mosquitoes per lamp·night), and in 2019, the proportion of Aedes albopictus in residential areas and hospitals was the highest (3.27% on average).The mosquito density in different habitats was the lowest in 2020. The proportion of Aedes albopictus in residential areas and livestock sheds showed an increasing trend from 2017 to 2021, while the proportion of other habitats showed significant fluctuations. The proportion of Aedes albopictus in hospitals showed an obvious decreasing trend in 2020. From 2017 to 2021, the average mosquito density was 5.97, 4.26, 3.82, 3.39 and 3.68 mosquitoes per lamp·night, respectively, showing a decreasing trend year by year except 2021. Except for 2019, the lure index also showed a decreasing trend, with an average value of 1.5/(hour·net), showing a decreasing trend year by year. From 2017 to 2021, the annual average density of Aedes albopictus in Hainan Province was 0.09 mosquitoes per lamp·night, showing obvious seasonal fluctuation. The Breteau index showed a slow decline from 2017 to 2021, and its annual peak was between June and August, which was 1-2 months later than the peak of adult mosquito density. Conclusions　The population density of Aedes albopictus in Hainan shows obvious seasonal characteristics. Mosquito control activities should be carried out according to the seasonal characteristics of mosquitoes to reduce the risk of dengue fever and other infectious diseases.

Objective　To explore the application of time series models based on meteorological factors in the population density of Aedes albopictus in Hainan Province, and to provide a scientific basis for the prevention and control of dengue fever in Hainan Province. Methods　The density of Aedes albopictus in different habitats in 18 cities and counties of Hainan Province from 2017 to 2022 was monitored monthly using the double-mosquito net trapping index and the Breteau index. Mann-Kendall trend test was used to analyze the temporal trends of the two density indices; Spearman's correlation analysis was employed to assess the correlation strength between each meteorological factor and the two indexes, eliminating unrelated variables, and further selecting the final variables through the full-subset regression method. Three time-series models were constructed for the two density indices, with root mean square error (RMSE), mean absolute error (MAE), and other accuracy metrics used to determine the optimal model; predictions for the density indices for 2023-2024 were made. All statistical analyses were performed in R (4.3.1). Results　The net trapping and Breteau indices showed an overall decrease over the years (Z-values of Mann-Kendall trend test were -6.15 and -4.03, respectively, and P<0.05). The meteorological factor most strongly associated with the trap index was the monthly average minimum temperature; monthly mean minimum temperature and monthly mean relative humidity were strongly correlated with the Breteau index. Based on various evaluation indicators, the multivariate time series model demonstrated the highest accuracy. The study predicts one to two peaks in both the trap index and Breteau Index for the years 2023 and 2024, with peak periods occurring between June to September and May to September, respectively. The predicted value for 2023 aligns with the measured value, demonstrating outstanding predictive accuracy. Conclusions　This study has introduced meteorological factors into the seasonal time series model, allowing for more accurate predictions of the density of Aedes albopictus in Hainan Province from 2023 to 2024, providing a model framework for the prevention and control of dengue fever in Hainan Province.

Background The diagnosis of central serous chorioretinopathy (CSC) is mainly dependent onfluorescine fundus angiography (FFA). However, the combination of optical coherence topography (OCT) with FFA offers a new approach to the research of the pathogenesis of CSC. Objective This clinical study was designed to study the combined application of the FFA and OCT for the research of the pathogenesis of central serous chorioretinopathy (CSC). Methods Forty-four eyes of 44 patients with CSC were included in this study with 36 cases of males and 8 cases of female. The patients were aged 39.3 ± 5.3 years and the visual acuity was 0. 64 ±0. 27. FFA and OCT examinations were performed in all patients and the FFA images were imported into the Topcon 3D OCT 1000 device to locate the conformity of OCT lesions with the leakages of FFA. The neuroepithelial layer thickness at the fovea and the height of the neuroepithelial layer detachment were measured using 3-D OCT. Results OCT showed serous REP detachment in 34 eyes (77.3%) and rough surfaces of RPE in 10 eyes (22. 7% ). In thirtyfour eyes with RPE detachment, the OCT lesions and FFA leakage spots conformed to the same locations in 31 eyes, but the other three eyes did not. The mean foveal neuroepithelial thickness was (138.5±19.4) μm in CSC eyes and that of normal eyes was ( 131.35±5. 01 ) μm ,showing a significant difference between them( t=0. 39 ,P＞0. 05 ). The mean height of neuroepithelial detachment was (263.3 ± 126.7 ) μm in CSC eyes. Conclusion RPE detachment occurs in CSC eyes and further induces macular neuroepithelial detachment. Leakage lesion of fluorescine corresponds to RPE detachment. CSC without RPE detachment may be related to the increase in RPE permeability. OCT can accurately measure the thickness of the macular neuroepithelial layer and the height of the neuroepithelial detachment.