2.ARDS in children: a prospective clinical study in four children's hospitals in Shanghai.
Zhu-jin LU ; Ying WANG ; Ding-hua TANG ; Yue-qun MI ; Bo SUN
Chinese Journal of Pediatrics 2003;41(8):619-620
Adolescent
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Child
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Child, Preschool
;
China
;
epidemiology
;
Female
;
Humans
;
Infant
;
Infant, Newborn
;
Intensive Care Units, Pediatric
;
statistics & numerical data
;
Lung Diseases
;
epidemiology
;
mortality
;
physiopathology
;
Male
;
Prognosis
;
Prospective Studies
;
Respiratory Function Tests
;
Survival Rate
3.Effect of Metalloproteinases Inhibitor on Expression of MMP-3 in Degenerated Lumbar Intervertebral Disc
De-sheng CHEN ; Qun-hua JIN ; Yan LI ; Wenjun YANG ; Xiaohong CHANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(8):692-693
ObjectiveTo explore the effect of metalloproteinases (MMPs) inhibitor on expression of MMP-3 in degenerated lumbar intervertebral disc.MethodsThe animal model of degenerated lumbar intervertebral disc was established with rats. The 32 rats were randomly divided into the experimental group and control group with 16 animals in each group. From the fourth week after operation, the animals of the experimental group were injected with tetracycline 25 mg per day, those of the control group with saline. Two weeks late, the tissue of degenerated lumbar intervertebral disc was taken and the expression of MMP-3 was tested by immumohistochemistry and Western-bloting.ResultsThe expression of MMP-3 in the experimental group decreased and significantly different from the control group ( P<0.05).ConclusionMMPs inhibitor can decrease expression of MMP-3 in degenerated lumbar intervertebral disc.
4.Historical evolution and development countermeasures of uncommon-territorial herbs.
Hua-sheng PENG ; De-qun WANG ; Jin-da HAO ; Jin XIE ; He-ling LIU ; Dai-yin PENG ; Lu-qi HUANG
China Journal of Chinese Materia Medica 2015;40(9):1635-1638
As an important part of Chinese medicinal materials, uncommon-territorial herbs are also the most complex parts in the herbal medicine markets. Through years of investigation on the key markets of Chinese herbal medicine, the meaning of uncommon-territorial herbs, their historical evolution, origin and characteristics were clarified in this paper, and some countermeasures were put forward for its development.
Biological Evolution
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China
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Drugs, Chinese Herbal
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chemistry
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history
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Herbal Medicine
;
history
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History, 20th Century
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History, 21st Century
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History, Ancient
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Medicine, Chinese Traditional
;
history
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Plants, Medicinal
;
chemistry
;
growth & development
5.A analysis on the effect of with or without patellar replacement in total knee arthroplasty.
Liang BAO ; Ji-hong HU ; Qun-hua JIN
Chinese Journal of Surgery 2012;50(2):171-175
OBJECTIVETo evaluate the outcome of total knee arthroplasty with or without resurfacing of the patella with particular attention to knee score, knee function score and incidence of postoperative anterior knee pain, providing the basis for the choice of surgical procedure.
METHODSCNKI, PubMed, ScienceDirect, Highwire and other databases were searched for the randomized controlled trials relevant to the patellar with or without replacement in total knee replacement arthroplasty between 1998 and 2010, evaluating of the methodological quality of included studies and extracting valid data.
RESULTSThe 80 citations were identified as related to patellar resurfacing during total knee arthroplasty, 13 articles meet all inclusion criteria for this study. The incidence of postoperative anterior knee pain is greater in knees without replaced patellas (RR = 0.78, 95%CI: 0.61 - 0.99, P = 0.04). No differences are observed between the 2 groups for knee score and knee function score. Knee score (WMD = -0.49, 95%CI: -1.79 - 0.81, P = 0.46), knee function score (WMD = 1.10, 95%CI: -1.77 - 3.98, P = 0.45).
CONCLUSIONSThe patella replacement can significantly reduce the incidence of postoperative anterior knee pain. There is no difference in the knee score and knee function score between two groups.
Arthroplasty, Replacement, Knee ; adverse effects ; methods ; Humans ; Knee Joint ; physiopathology ; Pain, Postoperative ; etiology ; Patella ; surgery ; Randomized Controlled Trials as Topic
6.Influence of glucocorticoid treatment on expressions of IL-12 and IL-13 in asthmatic children.
Yi-qun TENG ; Gui-zhi SHI ; Song-hua JIN ; Jingxiang YAO ; Lihua WANG ; Ping'an BI ; Zhigang WANG
Chinese Journal of Pediatrics 2003;41(1):53-54
Adolescent
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Asthma
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drug therapy
;
genetics
;
immunology
;
Child
;
Child, Preschool
;
Female
;
Gene Expression Regulation
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drug effects
;
Glucocorticoids
;
therapeutic use
;
Humans
;
Immunoglobulin E
;
blood
;
Infant
;
Interleukin-12
;
genetics
;
Interleukin-13
;
genetics
;
Male
;
RNA, Messenger
;
genetics
;
metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
7.Significance and role of increased expression of extracellular matrix metalloproteinase induced in the aseptic loosening of prostheses.
Qun-hua JIN ; Hou-shan LÜ ; Zhan-kun CHEN ; Dong-fang JIANG ; Ye-hua WANG
Chinese Journal of Surgery 2004;42(20):1232-1235
OBJECTIVETo investigate the expression of extracellular matrix metalloproteinase induced (EMMPRIN) in the interface tissue, and explore the role of EMMPRIN in the aseptic loosening of prostheses.
METHODSImmunohistochemistry was performed to characterize the EMMPRIN-expressing cells at sites of interface tissue around aseptic loosened hip prostheses in 16 cases. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to study the existence of EMMPRIN mRNA in interface tissue samples. And it was followed up by computer assisted image analysis in order to detect the A values of their expression. Synovium of hip joint of 8 femoral neck fracture were in control group.
RESULTSStrong immunostaining of EMMPRIN was found in the macrophages and fibroblasts of lining-like layers and vascular endothelium of synovial membrane-like interface tissue around loosened prostheses. Expression of EMMPRIN was significantly higher in interface tissue than the control synovium (z=-3.252, P=0.001). RT-PCR of interface tissue samples disclosed the presence of EMMPRIN mRNA of 14 cases. In interface tissue, the A value of EMMPRIN increased significantly compared to control synovium (P<0.01).
CONCLUSIONOver-expression of EMMPRIN up-regulates the production of matrix metalloproteinase (MMPs) in the interface tissue. And it can promote the bone destruction around prostheses. Thereby it may be one of methods to prevent and treat aseptic loosening of prostheses by repression the biology activity of EMMPRIN.
Adult ; Aged ; Arthroplasty, Replacement, Hip ; Basigin ; genetics ; physiology ; Female ; Hip Prosthesis ; Humans ; Immunohistochemistry ; Male ; Matrix Metalloproteinases ; metabolism ; Middle Aged ; Osteolysis ; physiopathology ; Prosthesis Failure ; RNA, Messenger ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Synovial Membrane ; metabolism
8.Lessons Learned from a Case with Valgus Deformity of the Knee Following Partial Removal of Lateral Discoid Meniscus.
Ming LI ; Hua LIU ; Zhi-Yong HE ; Zheng-Lin DI ; Jun-Hui ZHANG ; Qun-Hua JIN
Chinese Medical Journal 2015;128(21):2967-2968
Adult
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Cartilage Diseases
;
diagnosis
;
etiology
;
Female
;
Humans
;
Knee Joint
;
surgery
;
Menisci, Tibial
;
surgery
9.Evaluation of high-resolution images application for wild medicinal plants macro monitoring: a case of Apocynum.
Ren-Hua NA ; Jiang-Hua ZHENG ; Bao-Lin GUO ; Min-Hui SHI ; Ba-Ti SEN ; Zhi-Qun SUN ; Cheng-Zhong SUN ; Xiao-Jin LI ; Xiao-Guang JIA
China Journal of Chinese Materia Medica 2014;39(10):1777-1781
To investigate the resources of medicinal plant, such as wild Apocynum, supervised classification based on Principal Component Analysis (PCA) and texture feature were used to monitor wild medicinal plants from image captured by ZY-3 and World-view-2 and compare which satellite Image are more appropriate to monitor the wild medicinal plants. The research results shows that: for more complex growth conditions wild medicinal plants Apocynum, high-resolution images Worldview-2 is more suitable for its remote identification, the low-resolution satellite ZY-3 can only recognizes the wild medicinal plants which distributed intensively. If the study target distribution is more intensive and larger scale, and cultivated type medicinal plants, the use of satellite ZY-3 in low resolution remote sensing data to identify the target can be a good choice, it is not necessary to buy high-resolution data, in order to avoid waste of expenditure, for the scattered distribution, the high-resolution satellite imagery data may be indispensable to identify targets.
Apocynum
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chemistry
;
growth & development
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China
;
Conservation of Natural Resources
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Geographic Information Systems
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Plant Dispersal
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Plants, Medicinal
;
chemistry
;
growth & development
;
Remote Sensing Technology
;
methods
10.Establishment of a multiplex ligation-dependent SNP genotyping method and its application in the detection of genes related to chemotherapeutic drugs in breast cancer.
Jin-hai TANG ; Jian-hua ZHAO ; Jian-zhong WU ; Jian-wei LU ; Li-qun PAN ; Zhi-yin XU
Chinese Journal of Oncology 2009;31(2):108-113
OBJECTIVETo establish a method for SNP genotyping of multi-genes by allele-specific oligonucleotide probe ligation mediated by a thermostable ligase, and to explore the genetic polymorphisms of drug-metabolizing enzymes in breast cancer patients and their association with chemotherapeutic responses.
METHODS10 SNP loci of enzyme genes related to chemotherapeutic drugs such as taxanes, anthracyclines and cyclophosphamide were selected, and were genotyped for blood samples from 126 breast cancer patients by the established method. Their correlations with therapeutic responses were retrospectively evaluated.
RESULTSThe lower detection limit of genomic DNA by this developed method was 6.25 ng. The fluorescent peak locations of ligation products on ABI PRISM 377 DNA sequencer were accurate and consistent with prospective sizes in bases (Bias range 0.08 - 0.69 bp, x(-) = 0.31 bp, s = 0.18 bp). Same genotyping results were obtained for repeat tests of 8 random samples, which were further confirmed by sequencing analysis. The 10 SNP loci were polymorphic of different frequency in the breast cancer patients. The combinations with GSTP1 genotypes and GSTM1 genotypes were related to anthracycline-based chemotherapy efficacy (P = 0.037), and the low GSTs activity group (GSTP1 variant allele + GSTM1 null) showed the best effects (85.7%). GSTM1 genotypes and their combinations with GSTP1 and/or CYP3A5*3 genotypes were related to taxane-based therapy efficacy (P < 0.05 for all), and both the low GSTs activity group and the drug slow-metabolising group (low GSTs activity group + CYP3A5*3 wild allele) showed better effects (100%).
CONCLUSIONThe established method is reliable and applicable in multiplex SNPs genotyping of multi-genes. SNPs combination may have a better clinical application value for prediction of chemotherapeutic responses.
Adult ; Aged ; Anthracyclines ; therapeutic use ; Antineoplastic Agents ; therapeutic use ; Breast Neoplasms ; drug therapy ; genetics ; Cyclophosphamide ; therapeutic use ; Cytochrome P-450 CYP3A ; genetics ; DNA Mutational Analysis ; methods ; Female ; Gene Frequency ; Genotype ; Glutathione S-Transferase pi ; genetics ; Glutathione Transferase ; genetics ; Humans ; Middle Aged ; Polymerase Chain Reaction ; methods ; Polymorphism, Single Nucleotide ; Retrospective Studies ; Taxoids ; therapeutic use ; Treatment Outcome