Objective To investigate the relationship between cystatin C (CysC) and severity of the disease and changes of renal function in patients with acute cerebral infarction .Methods A total of 84 patients with acute cerebral infarction (study group) and 84 healthy individuals (control group) were enrolled and serum CysC levels were detected ,and its relationship with the degree of neurological impairment was analysed .500 mL/d (group A) and 375 mL/d (B group) 20% mannitol treatment were used ,and changes of serum CysC levels and renal function before treatment and 5 days and 14 days after treatment were analyzed .Results Serum CysC levels in patients with large ,medium and small infarcts were significantly higher than that in the control group (P<0 .05) .Serum CysC levels in patients with heavy ,medium and mild nerve function defect were significantly higher than that in the control group (P<0 .05) .After treatment ,serum levels of CysC ,creatinine (Cr) and blood urea nitrogen (BUN) in group A and B were increased ,in which serum CysC levels were significantly higher than that before treatment (P<0 .05) .5 and 14 days after treatment ,serum levels of CysC ,Cr and BUN in group A and B were without significant differences(P>0 .05) .Conclusion CysC might be closely related with the severity of acute cerebral infarction ,which could be more useful for monitoring renal function of patients with cerebral infarction than Cr and BUN .

Objective To investigate the clinical features,causes,pathogenesis and treatment of acute pancreatitis in patients with systemic lupus erythematosus (SLE).Methods From January 1983 to March 2003,15 SLE patients with acute pancreatitis were retrospectively analyzed.Results There were 12 patients with mild acute pancreatitis and 3 patients with severe acute pancreatitis.All patients received the treatment of moderate to large dosage of corticosteroids.Five patients recovered.Five patients improved.Five patients died.Conclusion (1)Acute pancreatitis occurs in the active stage of SLE.(2)The pancreatic vascular lesion is the main pathogenic mechanism in SLE acute pancreatitis.(3)The corticosteroids may not be the cause of SLE pancreastitis.(4)The treatment with moderate to high doses of corticosteroids is safe and effective.

The innovation of Chinese materia medica (CMM) is the central driver of CMM modernization. The application of modern theories and techniques in life sciences to innovating CMM research remains to be an urgent issue to be solved. Currently, the international R&D for drug is stepping into a new trend, in which these transformations are going through a signal target to multiple drug combination and active constituents selection by pharmacodynamics to optimize the design with pharmacokinetics. In the face of changes, the pace of CMM R&D is slow. Based on the R&D content of system biology and Huangqin Decoction extract (PHY906), the "preceded pharmacokinetics" concept is introduced. Taking pharmacokinetics as the tool to rapidly screen the potential constituents with the aims at CMM R&D. Furthermore, why the "preceded pharmacokinetics" is needed and how to start the innovation model are preliminarily discussed in this paper.

M2 type of pyruvate kinase (PKM2) is one of the most important regulatory molecules in glycolysis,which at high level is a major feature of tumor cells.Besides,PKM2 also regulates gene transcription and cell cycle,promoting the formation,invasion and migration of tumors.Meanwhile,PKM2 can beregulated by many transcription factors,oncogene proteins,miRNA and intermediate metabolites.Extensive studies indicated that PKM2 plays an important role in the developmental tumor.In view of the significance of PKM2 in tumor cells,it holds promise for diagnosis and treatment benefits.

Alveolitis, Extrinsic Allergic ; Female ; Humans ; Librarians ; Middle Aged

Adolescent ; Adult ; Burns, Chemical ; therapy ; Female ; Hand Injuries ; chemically induced ; therapy ; Humans ; Hydrofluoric Acid ; adverse effects ; Male ; Retrospective Studies ; Treatment Outcome ; Young Adult

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Idiopathic Pulmonary Fibrosis ; drug therapy ; Phytotherapy ; methods

[Objective] This study examined the distribution and the expression of platelet-derived growth factor-A (PDGF-A) in the ligamentum teres of children with developmental dislocation of the hip (DDH) and normal persons in order to investigate the roles of PDGF-A in hip joint laxity. [Methods]There were six pairs of joint laxity of children with DDH and normal children (control group) matched to gender and age. Immunohistochemistry (SP method) method was used to detect the location and distribution of the PDGF-A in the ligamentum teres,and to semi-quantify their content.Semiquantitative RT-PCR method was used to detect the PDGF-A in the ligamentum teres at mRNA level. The quantitative analysis of the PDGF-A was performed by professional image software and the results were analyzed with standard statistical methods.[Results]High density expression was observed in the synovial layer with fibroblast regularly arranged parallel to the joint surface in the ligamentum teres. PDGF-A distribution was decreased in the fibrous layer of the ligamentum teres. There were significant differences in the percentage of positive fibroblast and gray-scale density in the fibrous layer between DDH group and control group (P

Objective To explore and evaluate the predictive value of the coagulopathy in patients with community-acquired pneumonia (CAP).Methods A retrospective study was carried out for investigating the prothrombin time (PT),activated partial thromboplastin time (APTT),plasma fibrinogen (FIB),thrombin time (TT),plateslets (PLT),D-dimer in 385 patients with CAP and 146 patients without infection,tumor,trauma,thrombosis as controls.All the patients were admitted to the Respiratory Medical Department from June,2010 to May,2011.The results of the aforementioned biomarkers were analyzed and compared between two groups.The Pneumonia Severity Index (PSI) was calculated to evaluate the correlation between coagulopathy and PSI.Results The comparisons of the abnormal rates of PLT,PT,APTT,Fib,D-dimer between the patients with CAP and the controls were 92/385 vs.9/146,39/385 vs.1/146,108/385 vs.7/ 146,331/385 vs.47/146,348/385 vs.5/146,respectively.The differences were statistically significant (x2 =21.608,13.557,33.747,149.280,365.619,respectively,P ＜ 0.01),while difference in TT was not statistically significant (8/385 and 0/146,x2 =1.839,P ＞ 0.05).The differences in abnormal rate of PLT,PT,D-dimer between high-risk group of CAP and the low-risk group of CAP were 45/148 vs.47/237,26/148 vs.13/237,146/148vs.202/237,respectively,and the differences were statistically significant (x2 =5.602,14.609,23.442,respectively,P ＜0.05),while there were no differences in TT,APTT,FIB between two groups (6/148 vs.2/237,47/148 vs.61/237,123/148 vs.208/237,x2 =4.614,1.635,1.638,respectively,P ＞0.05).D-dimer in patients with CAP was (3.8 ±6.1) mg/L,compared with the controls (0.3 ±0.1) mg/L,and D-dimer in high-risk patients with CAP was (7.5 ±8.3) mg/L compared with the low-risk group (1.6 ±2.0) mg/L (P ＜ 0.001).Rank correlation existed between D-dimer and PSI (r =0.798,P ＜ 0.01),while there was no correlation between PLT and PSI (x2 =6.040,P ＞0.05).Conclusions The coagulopathy commonly occurs in patients with CAP.D-dimer was significantly higher in patients with CAP.D-dimer level is positively correlated with severity of CAP.D-dimer can be an ideal biomarker to assess the severity of patients with CAP.

Objective To establish the determination method for osthol in Compound Shechuang Tincture. Methods Hypersil ODS column was used and methanol-water (70∶30) was used as a mobile phase. The flow rate was at 1.0 mL/min and the detection wave-length was at 322 nm. Results Osthol had a good linear relationship between 0.018～0.126 ?g (r =0.999 9). The average recovery was 98.55% and RSD was 1.45%. Conclusion This method is simple, rapid and accurate. It can be applied to determining osthol in Compound Shechuang Tincture.