1.Apoptosis peripheral of T lymphocytes in patients with acute exacerbration of chronic bronchitis
Guo-Geng XU ; Qun MIU ; Jian-Ru HE ; Jun ZHOU ; Xiao-Qing CHEN
Journal of Zhejiang University. Medical sciences 2002;31(2):125-126
OBJECTIVE: To compare peripheral T lymphocyte apoptosis of chronic bronchitis during an acute episode with that during a stable period. METHODS: Seventeen Chronic Btonchitis patients who presented to the ER with an acute episode were compared with 10 stable Chronic Bronchitis patients seen in the out-patient clinic. Peripheral venous blood was obtained from each patient and T lymphocytes were separated by fluorescent monoclonal antibodies. CD3, CD95 and CD25 were measured by flow cytometry. RESULTS: In the acute episode patients, the total quantity of T lymphocytes with CD3 was (42.63 +/-16.37) and with CD95 (14.15 +/-11.06). In the stable group T lymphocyte CD3 was (64.22 +/-23.90) and CD95 (34.88 +/-8.92) (t=-4.238, P <0.001; t=-8.796, P <0.001). The total quantity of Lymphocyte CD25 did not differ significantly between the two groups (P > 0.05). Total quantity of active T lymphocytes with CD3/CD25 was lower in the acute episode group (6.26 +/-3.94) compared to the stable group (10.52 +/-3143) (t=-1.068, P <0.05). CONCLUSION: T lymphocyte apoptosis increased in Chronic Bronchitis patients who presented with an acute episode. They also experienced a significant disturbance in immune function.
2.Investigation of well-being index and self-efficacy among medical staff in different positions in military hospitals
Meng WANG ; Feifei LI ; Lu MIU ; Lin CHENG ; Qun YANG ; Xia WANG
Chinese Journal of Modern Nursing 2014;20(6):641-643
Objective To explore and analyze the difference of well-being index and self-efficacy among medical staff including military , inactive civilian and contract staff in different positions in military hospitals.Methods Totals of 1 851 medical staff from different positions of 13 military hospitals were investigated by the well-being index and self-efficacy questionnaire to compare the difference .Results The scores of military, civilian and contract staff in well-being index were (11.25 ±2.24), (10.67 ±2.12) and (10.09 ±2.43), respectively, which were lower than adult norm (11.81 ±2.23) with significant differences (t=-4.806, -7.999, -25.004, respectively;P<0.01).The scores of military, civilian and contract staff in self-efficacy were (2.46 ±0.60), (2.18 ±0.54) and (2.39 ±0.51), respectively, which were lower than adult norm (2.86) with significant differences (t =-12.910, -18.790, -32.788, respectively; P <0.01).The scores of well-being index and self-efficacy showed significant differences in military , civilian and contract staff (F=36.206,19.439,respectively; P<0.01).Conclusions Army medical staff’ s well-being index and self-efficacy are low , especially among civilian and contract staff .