1.Effect of Tiam 1 on cell proliferation and migration in head and neck squamous cell carcinoma cells.
Ping XIAO ; Quiyang FU ; Zeyong LI ; Xuehui ZHANG ; Xiangjun FU ; Renqiang WAN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(7):633-637
OBJECTIVE:
To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells.
METHOD:
Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay.
RESULT:
Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05).
CONCLUSION
Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
Blotting, Western
;
Carcinoma, Squamous Cell
;
metabolism
;
pathology
;
Cell Line, Tumor
;
Cell Movement
;
Cell Proliferation
;
Guanine Nucleotide Exchange Factors
;
genetics
;
metabolism
;
Head and Neck Neoplasms
;
metabolism
;
pathology
;
Humans
;
Squamous Cell Carcinoma of Head and Neck
;
T-Lymphoma Invasion and Metastasis-inducing Protein 1
;
Transfection