1.Mitochondrial mechanisms of antisense oligodeoxynucleotide Stat3 induced apoptosis in laryngeal carcinoma cell.
Haili LÜ ; Quihang ZHANG ; Bo YAN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(7):316-318
OBJECTIVE:
To investigate the changes of mitochondrion by transferring antisense oligodeoxynucleotide Stat3 into laryngeal carcinoma Hep-2 cell, for elucidating the mechanism of laryngeal carcinoma Hep-2 cell apoptosis, for developing more effective treatment for laryngeal cancer.
METHOD:
The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Mitochondrion membrane potential, VDAC and Cyt-c were detected for determining the changes of mitochondrion.
RESULT:
MMP was fell, Cyt-c and VDAC were increased with the heighten concentration of ASODN.
CONCLUSION
Mitochondria approach play an important role in the apoptosis mechanism of human Hep-2 cell by Stat3. This research elucidated the regulating mechanism of Hep-2 cell proliferation by Stat3, provided a new research focus for clinical therapy.
Apoptosis
;
Cell Proliferation
;
Cytochromes c
;
metabolism
;
Hep G2 Cells
;
Humans
;
Laryngeal Neoplasms
;
genetics
;
metabolism
;
pathology
;
Membrane Potential, Mitochondrial
;
Mitochondria
;
metabolism
;
Oligodeoxyribonucleotides, Antisense
;
genetics
;
STAT3 Transcription Factor
;
genetics
;
Transfection
;
Voltage-Dependent Anion Channels
;
metabolism