4.Methylation of PTPRG gene and its regulation in gastric cancer.
Chinese Journal of Oncology 2008;30(2):85-88
OBJECTIVETo investigate the difference in methylation of PTPRG gene between gastric primary cancer and its lymph node metastases, and its regulation by 5-Aza-2'-deoxycytidin in a gastric cancer cell line SGC7901.
METHODSMethylation-specific polymerase chain reaction (MSP) and RT-PCR were applied to identify the difference between gastric primary cancer and lymph node metastases and assess the changes of methylation in gastric cancer cell line SGC7901 treated by 5-Aza-2'-deoxycytidin.
RESULTSThere were significant differences of PTPRG gene methylation and PTPRG mRNA expression between gastric primary cancer and lymph node metastases: a linear regression analysis revealed a significant association between the quantity of metastatic lymph nodes and their methylation rate. A statistied relationship between methylation of PTPRG gene and loss of PTPRG mRNA expression was detected. PTPRG gene methylation in the gastric cancer cell line changed into negative and PTPRG mRNA expression in the cell line was recovered after 5-Aza-2'-deoxycytidin treatment.
CONCLUSIONThere is a difference of PTPRG gene methylation in gastric primary cancer and metastatic lymph nodes. 5-Aza-2'-deoxycytidin, an inhibitor of DNA methylation, can recovery the expression of PTPRG gene.
Azacitidine ; analogs & derivatives ; pharmacology ; Cell Line, Tumor ; DNA Methylation ; DNA Modification Methylases ; antagonists & inhibitors ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Lymph Nodes ; metabolism ; pathology ; Lymphatic Metastasis ; RNA, Messenger ; metabolism ; Receptor-Like Protein Tyrosine Phosphatases, Class 5 ; genetics ; Stomach Neoplasms ; genetics ; metabolism ; pathology
5.Detection of POU3F4 gene mutations in the Chinese pedigree with Y-linked hereditary hearing impairment.
Qui-Ju WANG ; Dong-Yi HAN ; Wei-Yan YANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(5):323-326
OBJECTIVETo analyze the mutations of candidate POU3F4 gene in the Chinese pedigree with Y linked hereditary hearing impairment.
METHODSPolymerase chain reaction (PCR) reactions were performed with five pairs of primer in the coding sequence of POU3F4 gene. PCR-single-strand conformation polymorphism (PCR-SSCP) was subsequently applied in the 43 individuals of DFNY1 family for screening the gene mutations.
RESULTSThe PCR amplification fragments showed well quality in the five pairs of primer and further analysis with PCR-SSCP showed no any polymorphism and mutations in the members.
CONCLUSIONSThe possibility of the deafness gene POU3F4, which locates on the translocation region on X and Y chromosome, contributed to the Y linked family deafness was successfully ruled out. It may imply that the causal gene of the DFNY1 family locate on the Y chromosome.
Adolescent ; Adult ; Aged ; Asian Continental Ancestry Group ; genetics ; Child ; DNA Primers ; Deafness ; genetics ; Female ; Genetic Diseases, Y-Linked ; genetics ; Humans ; Male ; Middle Aged ; POU Domain Factors ; genetics ; Pedigree ; Point Mutation ; Polymorphism, Single-Stranded Conformational ; Young Adult
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