Objective To determine the expression of sodium-hydrogen exchanger (NHE) 1 in gastric carcinoma tissue,and investigate the relationship between NHE1 expression and clinicopathological characteristics.Methods The expression levels of NHE1 mRNA and protein were detected in both gastric carcinoma tissue (48 cases) and paraneoplastic tissue (40 cases) by reverse transcription polymerase chain reaction (RT-PCR) and Western blot.The relationship between the expression levels of NHE1 mRNA and protein and the clinicopathological characteristics was analyzed.Results The relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.791 ± 0.286 and 1.475 ± 0.142,in paraneoplastic tissue were 0.352 ± 0.069 and 0.329 ± 0.048,and there were statistical differences (P ＜ 0.01).The expression level of NHE1 mRNA was positively correlated with NHE 1 protein in the gastric carcinoma tissue (r =0.375,P ＜ 0.05).The expression levels of NHE1 mRNA and protein were associated with the depth of invasion,lymph node metastasis and TNM staging (P ＜ 0.05).However,the expression levels of NHE1 mRNA and protein were no associated with age,gender and tumor differentiation (P ＞ 0.05).Conclusion The expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue,which may be involved in the development of gastric carcinoma.

Objective To investigate the apoptosis rate of neurons in vitro among the primary culture cells isolated from rat spinal cords before and after injury. Methods The spinal cord neurons of Wistar rats at 14-day gestation were isolated and cultured. The neuronal processes were then injured by cutting. At different time points after injury, TUNEL method was employed to detect the apoptotic neurons. Results Before injury, there was almost no apoptotic neuron. However, a large amount of apoptotic neurons were observed after the injury. The highest incidence of apoptosis appeared on the first day, and then it gradually reduced in the following days, but increased in amount again on the seventh day. Conclusion The results of the present experiment reveal the regularity of apoptosis of neurons before and after injury, and it provides a platform for further research regarding therapeutic intervention in the treatment of spinal cord injury.

Acupuncture Therapy ; Aged ; Combined Modality Therapy ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Osteoarthritis, Knee ; therapy

Objective To observe the effect of Tiaowei Xiaozhi Pills on gastric juice secretion and gastrointestinal function in rats model of damp stagnation syndrome. Methods The damp stagnation syndrome model was established in rats. The gastric juice,pepsin and gastrointestinal function were determined. Results Tiaowei Xiaozhi Pills in the dosages of 700 mg/kg and 350mg/kg can diminish the amount of gastric juice and pH level and increase pepsin level. Tiaowei Xiaozhi Pills in the dosages of 700 mg/kg,350 mg/kg,175 mg/kg can also improve gastrointestinal function in model rats. Conclusion Tiaowei Xiaozhi Pills have improvement on gastrointestinal function in rats model of damp stagnation syndrome.

Objective To investigate the clinical efficiency,safety,adverse reactions and healthrelated life quality of transdermal fentanyl patches for pain management in terminal cancer patients with hepatic and renal dysfunction and ascites.Methods 98 terminal cancer patients with moderate to serve pain combined with abnormal hepatic and renal dysfunction were randomly divided into two groups.Group Ⅰ (n=56)received transdermal fentany patches.Group Ⅱ,the control group (n=42),received morphine controlledrelease tablets.Terms of pain intensity,function of renal and liver,adverse reactions and heath-related quality of life were assessed before and after treatment.Results Patients were satisfied with their pain management in both groups,and no significant difference in pain release was observed between the groups (x2 =0.01,0.07,0.01,0.04,P ＞ 0.05).The incidences of constipation and dysuresia were significantly less in the group Ⅰ compared with that of the group Ⅱ (x2 =7.32,3.96,P ＜ 0.05).The incidences of hypersomnia,dizzy,nausea and vomiting were similar betwecn the two groups (x2 =0.12,0.54,0.54,0.02,P ＞ 0.05).Most of the adverse reactions would relieve or disappear after symptomatic treatment.Values of BUN,CR,ALT,AST were similar before and after treatment in group Ⅰ (t =1.43,1.67,0.91,0.11,P＞ 0.05).However,in group Ⅱ,these values were significantly increased after treatment (t =17.59,49.17,42.12,36.23,P ＜ 0.05).The heathrelated life quality (appetite,spirit,somnus,fatigue,daily life and countenance) were significantly improved after treatment in both groups (the group Ⅰ t =3.37,4.40,2.07,5.66,4.48,P ＜ 0.05; the group Ⅱ t =2.03,2.27,3.59,4.16,2.79,P ＜ 0.05),the spirit group Ⅰ improved more obviously compared with group Ⅱ (t =2.93,P ＜ 0.05).Conclusion Transdermal fentanyl patches provides equal pain relief compared with Morphine controlled-release tablets in the terminal cancer patients with abnormal hepatic and renal function and ascites.Transdermal fentanyl patches significantly improve health-related quality with less hepatic and renal function influence and low incidence of adverse reaction.It should be recommended in the treatment terminal cancer patients with moderate to serve pain combined with abnormal hepatic and renal function and ascites.

To explore the clinical manifestation of lymphomatoid granulomatosis (LG) and the early diagnosis. Retrospective analyses of the clinical features of LG and pathology features by open lung bio-psies were conducted. The patient was a 42-year-old female with irregular fever,and her chest X-ray and computed tomography showed nodules with cavity and pleural effusion. Open lung biopsy proved LG. LG is seldom seen in clinic. Open lung biopsy is very important for pathology diagnosis. Early diagnosis and treatment are the key to improving survival in these patients. The therapeutic effect is good.

The thread-dragging therapy, as a new external treatment of traditional Chinese medicine, could eliminate pathogenic factors and restore the vital qi by promoting qi and activating blood circulation to advance tissue repair in treating refractory sinus tracts or fistulae. This article reviewed its origin and development, and introduced its use in treating refractory sinus tracts or fistulae.

Objective To investigate the influence of psychological intervention on the physical and psychomotor development of children born of assisted reproductive technology. Methods 329 cases of ART born children in our hospital were set as the experimental group,the parturients were given pertinent psychological intervention.254 cases of children of spontaneous pregnancy were selected as the control group.The two groups were given follow-up for 24 months.Their physical condition at birth and psychomotor development during follow - up period were compared. Results Compared with the control group,length of infants at birth,body mass of the experimental group were significantly lower,and the incidence rate of premature birth,low birth weight,NICU stay during neonatal stage were higher.But there were no differences in psychomotor development between two groups. Conclusions ART born children of 24-month old show normal psychomotor development,but there are still more unfavorable physical factors,therefore,pertinent nursing intervention should be given based upon psychological intervention in clinic.

Traditional Chinese Medicine (TCM) therapy is effective in treating malignant tumor. TCM therapy is a multi-way, multi-layer and multi-target integrated treatment characterized by "survival with tumor" and improvement of life quality. An ideal result of the TCM therapy for cancer should be a positive response of the patient with satisfactory quality of life (QOL) and longer survival time. So it is necessary to introduce the QOL, quantification of symptoms and signs and standardization of TCM syndromes to the objective, quantitative and standardized effect assessment of TCM therapy for cancer. A criterion for evaluating the effect of TCM therapy on tumors should be established, which reflects the characteristics and advantages of the TCM therapy, and meets the requirement of the developing effect assessment of modern medicine in cancer treatment. It is suggested that the treatment effect should be the sum of quality adjusted life years (QALYs) (equal to QOL multiplied by time of survival), syndrome remission rate and tumor remission rate. But the proportion of each of the three is variable, which has close relationships with the different tumor stages and corresponding treatments.