Aim To investigate the effect of U50488H(a selective κ-opioid receptor agonist)and isoproterenol(ISO,a β-adrenergic receptor agonist)on ventricular arrhythmias and Cx43 during myocardial ischemia and reperfusion in rats.Methods 60 rats were randomly divided into five groups,ie,normal control group,I/R group,ISO+I/R group,U50488H+ISO+I/R group,Nor-BNI+U50488H+ISO+I/R group.The incidence of ventricular arrhythmias and arrhythmia score were determined. The expression of Cx43mRNA was tested by RT-PCR.The expression of Cx43 protein in myocardial cell was tested by an immunohistochemical approach with a quantitative imaging system.Results ① Compared with the I/R group,arrhythmia score was increased with administration of ISO(P<0.05).U50488H intravenously injected before ISO significantly decreased the arrhythmia score(P<0.05).② Compared with the normal control group,the expression of Cx43 mRNA was decreased in the I/R group(P<0.05).With administration of ISO,the amount of Cx43 mRNA was not significantly increased.③ Compared with normal control group,total and phosphorylated Cx43 proteins were significantly decreased in the I/R group(P<0.05),and the phosphorylated Cx43 was also decreased with administration of ISO.Compared with ISO+I/R group,phosphorylated Cx43 was increased with administration of U50488H (P<0.05).Conclusion κ-opioid receptor agonist U50488 H antagonizes the arrhythmias through the regulation of Cx43 during myocardial ischemia and reperfusion via inhibiting β-adrenergic receptor pathway.

200 papers on nerve root type cervical spondylosis treated with Chinese medicine were retrieved and 38 papers with complete diagnostic criteria and medical statistics were included for study. The results showed acupuncture, massage, and herbal therapy were three common methods and have their own advantage, but systemic, standardized and normative treatment program was lack. In the meantime of treating nerve root type cervical spondylosis, prevention should also be paid attention. The treatment, prevention and exercise on the whole therapeutic idea should be established, which has far-reaching significance.

Objective　To evaluate the anti-tumor effects of NDV and two genes of virus(HN and F) on athymic mice with human adenocarcinoma xenografts, and to investigate the mechanisms of its oncolytic role. Methods　The experimental model of lung adenocarcinoma xenograft was established. The two experimental groups of athymic mice were given intratumoral injections of NDV and plasmids only once, and compared with PBS controls in the same time. Measure the volume of tumors for 5 weeks and make a curve of the volume. These mice were killed after 5 weeks, and the weight of the tumors was measured. The histological and ultrastructral changes were observed by electromicroscope and microscope. Results　After one injection of live NDV and plasmids, the tumor growth was significantly suppressed (The median inhibitory rate was 71.62％ and 79.40％ respectively). The median weight of tumor of mice treated with NDV was remarkably lower than that of mice treated with PBS, and that of the mice treated with plasmids (P＜0.01). 14％ of the control group had liver and lung metastasis of the tumor, but no metastasis was found in the experimental groups. A great quantity of NDV viron budding was found in the NDV group. Conclusion　NDV could replicate in human lung adenocarcinoma xenografts, leading directly to a potent anti-tumor effect after one injection of live NDV. During the oncolytic process, the gene HN and gene F may play an important role.