Nonaqueous capillary electrophoresis-chemiluminescence (NACE) method was developed for the determination of five phenolic environmental estrogens including bisphenol A (BPA),4-nonylphenol(4-NP) ,4-tert-octylphenol(4-tOP),4-tert-butylphenol(4-tBP) and tetrabromobisphenol A(TBBPA) in food packaging material. Target compounds in soaking solution of food packaging material samples were separated by NACE-CL after derivatization with 4-(4,5-diphenyl-lH-imidazol-2-yl) benzoyl chloride ( DIB-Cl) ,reacted with per-oxyoxalate chemiluminescence system,and then detected through magnifying light signal with photomultiplier. With 17β-E_2 as internal standard,qualitative and quantitative analysis of the target compounds were performed by relative migration time and the ratio of relative chemiluminescence intensity,respectively. Several influence factors on separation with NACE,such as the composition and proportion of organic solvent,the concentration of electrolyte,temperature surrounding of the capillary,the concentration of acetic acid and work voltage,were investigated. And the peroxyoxalate chemiluminescence system was optimized. Under these conditions,4-tBP,BPA,4-OP,4-NP and TBBPA were all separated and showed good linearities in the ranges of 0.0095-3.0 mg/L,0.0087-3.0 mg/L,0.0085 -3.0 mg/L,0.011 -3.0 mg/L,0.009-3.0 mg/L,respectively,with correlation coefficients over 0. 9947. The RSDs of relative migration time and relative chemiluminescence intensity ratio were 0.9% -3.0% and 6.5% - 8.6%. Recoveries for the spiked samples ranged from 86.7% to 98. 8%. Five common food packaging material samples were analyzed. The proposed method is simple and sensitive for the quantitative determination of phenolic environmental estrogens in food packaging material samples.

OBJECTIVE:To optimize water reflux extraction technology of Jiuwei yiwei mixture. METHODS:With the over-all score of the content of salvianoli acid B,a water-soluble component of the principal drug Salvia miltiorrhiza,the content of pae-oniflorin in Paeonia Radix Rubra and dry ointment yield rate as the observed index,and taking soaking time,extraction time, amount of water added,and extraction times as the factors,L9(34)orthogonal test was designed to optimize the extraction technolo-gy of Jiuwei yiwei mixture. And the verification test was conducted. RESULTS:The optimal technology was to add water with an amount 8 times as much as that of the drugs to soak them for 1 h and to conduct extraction twice,1 h for each. The overall scores of 3 batches of samples in the verification test were respectively 98.793,99.078 and 99.220(RSD<2%,n=3). CONCLUSIONS:The optimal technology for Jiuwei yiwei mixture is stable and feasible.

Objective:To determine the release and in vitro transdermal rate of tetrahydropalmatine in Xiaoji Aitong cataplasmas in rats. Methods:Using the self-made drug release determination devices and the modified Franz diffusion cells, and the skin of rats as the barrier, the release and in vitro transdermal rate of tetrahydropalmatine in Xiaoji Aitong cataplasmas were detected by HPLC. Re-sults:Tetraydropalmatine within the range of 0. 51-10. 22 μg showed a good linearity (r=0. 999 7), and the average recovery was 98. 49%(RSD=0. 84%,n =9). The release of tetrahydropalmatine in 80 min was 63. 60%, and the skin permeation rate was 23. 15% in 24h. Conclusion:Xiaoji Aitong cataplasmas have good drug release and transdermal performance.