Objective To study the correlation between the genes of killer immunoglobulin (Ig)-like receptors (KIR) in natural killer cells and leukemia. MethodsPrimers of 16 KIR genes, including KIR2DL1-5, KIR3DL1-3, KIR2DS1-5, KIR3DS1 and 2 pseudogenes 2DP1 and 3DP1, were designed and synthesized. A polymerase chain reaction with sequence-specific primers (PCR-SSP) was developed to detect the mRNA expressions of the 16 genes. Totally 46 healthy individuals and 46 leukemic patients (15 cases of acute myelocytic leukemia, 13 cases of chronic myelocytic leukemia and 18 cases of acute lymphocytic leukemia) were detected and the frequencies of the KIR genes were analyzed. ResultsThe frequency of 2DL4, 3DL2, 3DL3 and 2DP1 in healthy and leukemic patients were 100%, and were detected in all patients and healthy individuals. The other KIR genes had similar frequencies except those of 2DL2 and 2DS2, which were 47.8% of the healthy individuals and 89.1% in leukemic patients. There was a significant difference between the 2 groups.ConclusionThere may be an association between pathogenesis of leukemia and 2DL2 and 2DS2 genes.

Objective To observe the clinical efficacy of minimally invasive percutaneous K-wire crossover fixation treating scaphoid fractures under C-arm.Methods From March 2011 to May 2014,20 patients with fresh scaphoid fractures had postoperative regular outpatient follow-up.Evaluation indicators included Cooney wrist scoring system,X-ray assessment of fracture healing,questionnaire survey of patient's subjective satisfaction at the final follow-up.Results All patients were followed up for 4 to 18 months,the mean follow-up time being 10 months.Postoperative X-ray showed all cases achieved bony union within 8 to 12 weeks,an average of 9.2 weeks.The questionaire resuits of patient's subjective satisfaction showed that general feeling of treatment in 2 cases,relatively satisfied in 2 cases,greatly satisfied in remaining 16 patients at the final follow-up.According to the postoperative Cooney wrist score,excellent in 8 cases,good in 10 cases,fair in 2 cases,the overall satisfactory rate was 90.2％.There were not pin tract infection,nonunion,malunion,avascular necrosis of scaphoid and other complications.2 cases showed that wrist pain during weight-bearing activities and weather getting cold,minor limitations of wrist function and degree of activity,grip strength slightly worse than the contralateral.Conclusions Minimally invasive percutaneous K-wire crossover fixation under C-arm combined with postoperative plaster external fixation properly can attain good clinical results in the treating of scaphoid fractures and worthy of further promotion.

This paper is about the research on the growth of jyphal body of Hypsizygus marmoreus under the condition of different culture mediums, tempertures, pH, cardon and nitrogen sources. The result shows that Hypsizygus marmoreus can grow on various culture mediums. It grows best on the Culture medium of malt extract agar, but can not grow well on the PDA composite culture medium; the hyphal body can grow well at the temperture of 20—25℃, grows slowly at 10—30℃, but can not grow at 35℃; it can grow when pH is 5 to 8, but the best range of pH is 6.5—7.0; the nutriment assimilation is different with the different sources of carbon and nitrogen, the best carbon source is maltose, best nitrogen source is bacto-peptone.

Objective To explore the safe bone channels for transverse placement of sacroiliac screws via the second sacral vertebra in the fixation of longitudinal fracture of the sacrum by digital analysis and clinical observation.Methods Firstly,abdomen CT scanning was performed in 50 healthy adult volunteers.They were 30 males and 20 females,aged from 18 to 56 years (mean,34.6 years).After their CT images were transformed by software Materialise Mimics Innovation Suite 15.0,three-dimensional (3D) models of the pelvis were generated,stored in stereolithography format,and transferred into software Imageware 12.0.In the standard antero-posterior position,the sacrum was segmented and the points of 2D image coordinate geometric boundary were extracted to generate an optimal channel for transverse placement of sacroiliac screws via the second sacral vertebra.Secondly,we admitted 10 patients with longitudinal fracture of the sacrum.They were 7 men and 3 women,aged from 20 to 51 years (average,38.3 years).By Tile classification,4 cases were type B2 and 6 type C1.All the fractures were Denis region Ⅱ ones.Pubic ramus fracture was complicated in 3 cases.After traction reduction of the tibial tubercle was conducted for patients with obvious displacement,the optimal channel was calculated on the basis of the CT data.On the lateral images of the sacrum acquired before operation by C-arm fluoroscopy,the optimal channel for transverse placement of sacroiliac screws via the second sacral vertebra was located.After the skin was marked,2.5 mm Kirschner wire was drilled and the 6.0 mm hollow screws were fixated along the Kirschner wire.Postoperative CT scanning images and 3D reconstructed models were analyzed to validate the accuracy of screw placement.Results The projection of safety bone channel on the sagittal fluoroscopy of S2 vertebral body displayed an irregular water drop shape;the projection area in males (213.9 ± 52.4 mm2) was significantly larger than that in the females (171.6 ±49.6 mm2) (P ＜ 0.05).The average length of the channel in men (135.2 ± 12.9 mm) was significantly longer than that in women (121.1 ± 10.1 mm);the average diameter of the screw in men (10.2 ± 0.9 mm) was also significantly larger than that in women (9.1 ±0.8 mm) (P ＜ 0.05).The postoperative X-ray and CT scanning images showed satisfactory positions and lengths of the screws.The screw lengths averaged 98.2 mm;the operation time averaged 25.6 min.No nerve or vascular injury,loosening or breakage of the sacroiliac screws occurred in the patients.Follow-ups for 12 to 24 months revealed no other complications.Conclusion The safety channel for transverse placement of sacroiliac screws via the second sacral vertebra can be determined using the lateral 3D reconstruction images of the sacrum in preoperative planning,which facilitates the percutaneous transverse fixation of longitudinal fracture of the sacrum.

OBJECTIVE To compare two different procedures of blood donation in volunteer donors,which lead to different discard rates of blood,different donation reaction rates and the satisfaction of the donor agency,so as to seek the better procedure bringing less discard of blood and more convenience for the military donor agency and blood center. METHODS In group A,3 667 donors blood was collected before the tests and retests for transfusion transmitted diseases(TTD) were done.While in group B,4 185 donors were taken blood samples for pre-donation test.The blood collection was performed 4 hours later. RESULTS In group A,3 652 units of blood were collected,of which 69 units were discarded on account of positive results in test and retest.Meanwhile,in group B 3 718 units of blood were collected from the donors who passed the pre-donation test for TTD.As a result,34 units of blood were discarded because of the positive results in retest.The discard rates of blood were 1.89% and 0.91% while the donation reaction rates were 2.22% and 3.98%,respectively.in two procedures.The discard rates of blood in group A were higher than those in group B.But the donation reaction rate in group B was higher than that in group A. CONCLUSIONS The discard rate of blood in the procedure collecting before test is higher than that in the procedure testing before collection.But the donation reaction rate is low and the waiting period for donation is short in the former procedure,which is suitable for low TTD infections population of military agencies.

Objective To study and analyze value of the hemodynamic monitoring in the differential diagnosis of severe sepsis and acute heart failure.Methods A total of 86 patients with severe sepsis and acute heart failure treated with hemodynamic monitoring of PICCO were selected as the research subjects,among whom 55 patients were severe sepsis,and 31 patients were acute heart failure.The cardiac index (CI),the right atrial pressure (RAP),the pulmonary artery wedge pressure (PAWP),the mean arterial pressure (MAP),the heart rate (HR) of the patients were detected and compared.Results The RAP,PAWP levels of the patients with acute heart failure were significantly higher than that of the patients with severe sepsis.The CI level was significantly lower than that of the patients with severe sepsis,and the differences between the two groups were statistically significant (P ＜ 0.05).The area of the receiver operating characteristics curve (AUC) of CI in the differential diagnosis of severe sepsis and acute heart failure was the highest,which was 0.893.At the optimal screening limit value (Cut off value),the sensitivity and the specificity were 0.764 and 1.000.The AUC of RAP or PAWP in the differential diagnosis of severe sepsis and acute heart failure were lower than that of CI,the sensitivities at Cut off value were relatively lower,which were 0.323 and 0.484,respectively.Conclusion The invasive hemodynamic monitoring indicators of the patients with severe sepsis and acute heart failure have certain differences.Monitoring indicators can provide the basis for the early differential diagnosis and the development of treatment strategy,which is beneficial to improve the therapeutic effects and the prognosis of the patients.

Objective To study and analyze value of the hemodynamic monitoring in the differential diagnosis of severe sepsis and acute heart failure.Methods A total of 86 patients with severe sepsis and acute heart failure treated with hemodynamic monitoring of PICCO were selected as the research subjects,among whom 55 patients were severe sepsis,and 31 patients were acute heart failure.The cardiac index (CI),the right atrial pressure (RAP),the pulmonary artery wedge pressure (PAWP),the mean arterial pressure (MAP),the heart rate (HR) of the patients were detected and compared.Results The RAP,PAWP levels of the patients with acute heart failure were significantly higher than that of the patients with severe sepsis.The CI level was significantly lower than that of the patients with severe sepsis,and the differences between the two groups were statistically significant (P ＜ 0.05).The area of the receiver operating characteristics curve (AUC) of CI in the differential diagnosis of severe sepsis and acute heart failure was the highest,which was 0.893.At the optimal screening limit value (Cut off value),the sensitivity and the specificity were 0.764 and 1.000.The AUC of RAP or PAWP in the differential diagnosis of severe sepsis and acute heart failure were lower than that of CI,the sensitivities at Cut off value were relatively lower,which were 0.323 and 0.484,respectively.Conclusion The invasive hemodynamic monitoring indicators of the patients with severe sepsis and acute heart failure have certain differences.Monitoring indicators can provide the basis for the early differential diagnosis and the development of treatment strategy,which is beneficial to improve the therapeutic effects and the prognosis of the patients.

OBJECTIVETo develop an oral DNA vaccine based on MG(7)-Ag mimotope of gastric cancer using attenuated Salmonella typhimurium and evaluate its efficacy and protective effect.

METHODSThe eukaryotic expression vector including the MG(7)-Ag mimotope and a Th epitope was constructed, and then transduced into an attenuated Salmonella typhimurium to get the oral DNA vaccine. C57BL/6 J mice were orally immunized with 1 x 10(8) cfu Salmonella transfectants, with Salmonella harboring empty plasmid, with phophate buffered saline (PBS) as control. At the 6th week, serum titer of MG(7) antibody was detected by ELISA. In the 8th week, a [(3)H]-thymidine incorporation assay was performed to test the proliferation of murine spleen cells to the stimulant of MG(7)-Ag mimicry peptide. At the same time, Ehrlich ascites carcinoma cells expressing MG(7)-Ag were used in tumor challenge assay to evaluate the protective effect of the immunization.

RESULTSThe oral DNA vaccine induced MG(7) antibody in mice, while in vivo unprimed proliferation assay of the spleenocytes showed no difference among the three groups. Two weeks after tumor challenge, 2 in 7 immunized mice were tumor free, while none in the control group was protected.

CONCLUSIONOral DNA vaccine based on the MG(7)-Ag momitope is immunogenic. It is able to induce specific immunity response against tumor in mice, and the vaccine is partially protective.

Administration, Oral ; Amino Acid Sequence ; Animals ; Antigens, Neoplasm ; blood ; genetics ; immunology ; Base Sequence ; Cancer Vaccines ; genetics ; immunology ; therapeutic use ; Epitopes ; genetics ; immunology ; Female ; Mice ; Mice, Inbred C57BL ; Molecular Mimicry ; genetics ; immunology ; Molecular Sequence Data ; Plasmids ; genetics ; Polymerase Chain Reaction ; Stomach Neoplasms ; drug therapy ; immunology ; Treatment Outcome ; Vaccines, DNA ; genetics ; immunology ; therapeutic use

OBJECTIVETo synthesize and evaluate a novel injectable and water-swelling gingival displacement materials.

METHODSA kind of water-swelling polymer, kaolin and aluminum chloride were mechanically mixed at certain ratio in water solution, resulting to a novel paste materials for gingival displacement. Then, its stability in aqueous solution and water swelling properties were evaluated in vitro. The effect on gingival displacement was evaluated by animal experiments in dogs. A commercial gingival displacement materials paste of Expasyl was used as control.

RESULTSWhile contacting with water, the novel gingival displacement paste did not collapse, maintained its integrity structure, and could expand for adsorbing water. Animal experiments in dogs showed that the materials could lead to displace the gingival margins from the dental root surfaces.

CONCLUSIONThe novel injectable and expanded gingival displacement material is efficient to retract free gingival margin with potential clinical application.

Aluminum Compounds ; Animals ; Chlorides ; Dogs ; Gingiva ; Water

OBJECTIVETo design a kind of biomimetic polypeptide of dentin matrix protein-1 (DMP-1), which can bind to dentine collagen fibers and initiate mineralization.

METHODSA novel polypeptide was developed by connecting the collagen binding domain of DMP-1 "DSESSEEDR" to the hydrophilic C-terminal of amelogenin "TKREEVD". The polypeptide was synthetically prepared by standard solid-phase peptide synthesis. Human dentine slices were completely demineralized by hydrochloric acid to expose the dentine collagen. Fluorescein isothiocyanate coupled polypeptide was applied to the exposed dentine collagen. Fluorescent microscopy was used to examine the polypeptide specially bond to the dentine collagen. Nucleation and growth of hydroxyapatite was initiated by immersing the polypeptide into calcium chloride and sodium hypophosphate solutions respectively. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and selected area diffraction (SAD) were used to examine the hydroxyapatites formed. RESULTS Fluorescent dentine collagen was identified in the demineralized dentine specimens. Nucleation and growth of crystals were detected after immersing the polypeptide into calcium chloride and sodium hypophosphate solutions by SEM and TEM. SAD confirmed the crystals were hydroxyapatites.

CONCLUSIONThe polypeptide of "DSESSEEDRTKREEVD" can simulate DMP-1 binding collagen and initiate hydroxyapatite nucleation and growth. It may be a potential molecular tool for dentine remineralization.

Biomimetics ; Calcium Phosphates ; Collagen ; Dentin ; Durapatite ; Humans ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Peptides