Objective To investigate the level of MEF2C phosphorylation (activation) and protein expression, and to further clarify the possible mechanism following ischemia-reperfusion in hippocampal CA1 region of rat. MethodsBrain ischemia was induced by four-vessel occlusion in SD rats. Protein level was determined by Western blotting. Results MEF2C was significantly activated with a peak at 6 h of reperfusion, but its protein expression decreased in late phase of reperfusion (3～5 d). The elevation of activated (17 ku) and the inactivated forms (32 ku) of caspase-3 proteases were remarkable during 1～5 d of reperfusion. In addition, Ac-DEVD-CHO, a specific inhibitor of caspase-3, up-regulated MEF2C protein level of 3 d reperfusion. SB202190 (an inhibitor of P38), but not ERK5-antisense oligonucleotides, not only inhibited MEF2C activation of 6 h reperfusion but also apparently prevented the increase of caspase-3 activation caused by 3 d reperfusion. Conclusion P38/caspase-3 mediated MEF2C pathway may function in the injuries of hippocampal CA1 region of rats following ischemia/reperfusion.

Objective To study the methods of three dimensional reconstruction of digitized virtual breast invasive ductal carcionma and sterical characteristic of tumor growth.Methods The specimens were sliced into 0.2 mm continuous sections on transversal plane with the computerized miller,and photographs were taken with digital camera.3DDOCTOR software was used to make three dimensional block diagram and the thin sectional and three dimentional characteristics of breast invasive ductal carcionma were observed.Results Every structure of breast in thin section was clear.Stereo image of breast solid could be shown satisfactorily.Every shape of stereo image and the structure of breast could be shown by revolving the three dimensional image in different direction,beneficial to us in understanding the dimensional growth pattern of tumor.Conclusion The sliced image of breast invasive ductal carcionma database was exact.The image of three dimentional reconstruction was clear,which could reflect the structural feature and growth pattern of breast carcinoma in the breast exactly and be conducive to the evaluation of tumor margin in breast conservation therapy.

Objective To study on how to remove the background in the digital images of visible human body.Methods After removing most of the background with standardized thresholds segmentation,we did identification,connection and noise-removing work against the edge around the target verge,and then the results of these two methods were combined to get the final image.Results Complex segmentation technology based on edge and thresholds effectively removed the background in the image of human body.Conclusion Compared with other segmentation algorithm,the algorithm presented here has better practical effect.

Objective To evaluate ultrasound-guided posterior approach to intercostal block for herpetic neuralgia. Methods Forty-eight patients with herpetic neuralgia after appearance of rashes on the back of chest (the coursc ＜ 30 days) aged 56-84 yr received intercostal block performed via posterior approach under the guidance of ultrasound with a mixture of 0.75% ropivacaine, glucocorticoid and methylene blue. Pain was assessed with visual analogue scale (VAS) before block and at 1, 2, 4 and 8 weeks after block. Pain relief (PAR) was cal-culated (PAR= (VAS score before block- VAS score after block) ÷ VAS score before block × 100%). Results No patient developed dyspnea and pneumothorax. VAS scores were reduced significantly, quality of life was improved and PAR increased at 1, 2, 4 ancl 8 weeks after block ( P ＜ 0.05 or 0.01). Concluslon Ultracound-guided posterior approach to intercostal block is safe and effective for the treatment of herpetic neuralgia.

Objective To reconstruct the Chinese visible human (CVH) dataset with the reconstruction algorithm, the Visualization Toolkit (VTK), and to realize long-distance accessing and alternating operation associated with virtual reality modeling language (VRML) under web condition. Methods 3D structure of the humerus was reconstructed and put out in web condition by applying the methed of capturing data of digitalized visible human. Results The 3D structure of the humerus was successfully reconstructed. These 3D images displayed the anatomical relationships of the structures of the humerus in explore windows with any angle and scale. All reconstructed structures were represented conveniently. Conclusion Based on VTK and VRML, 3D reconstruction of the humerus is realized, which will provide the support and reference for further research on the others structure's visualization of human body, and also for Visualization in Scientific Computing and long-distance teaching in anatomy.

Objective To evaluate the efficacy of continuous lumbar plexus block (CLPB) combined with a bolus dose added at night for postoperative analgesia in patients undergoing hip arthroplasty.Methods Sixty ASA Ⅰ or Ⅱ patients of both sexes,aged 51-75 yr,weighing 47-77 kg,with body height 150-180 cm,scheduled for hip arthroplasty,were randomized to receive either CLPB (group CLPB) or patient-controlled intravenous analgesia (PCIA) (PCIA group) for postoperative analgesia (n =30 each).Spinal anesthesia was performed at L3,4 interspace.Postoperative analgesia was performed at 30 min before the end of surgery.PCIA solution contained morphine 100 mg in 100 ml of normal saline.The PCA pump was set up with a 2 mg bolus dose and a 5 min lockout interval.CLPB solution contained 0.125 ％ ropivacaine hydrochloride 200 ml.CLPB pump was set up to deliver a 4 ml bolus dose with a 30-min lockout interval and background infusion at 8 ml/h after a loading dose of 0.125％ ropivacaine 30 ml.In addition the patients received 0.25％ ropivacaine 30 ml at 8 o' clock every night after surgery in group CLPB.VAS scores at rest and during activity were recorded at 6,12,18,24,30,36,42 and 48h after operation.The side effect such as nausea and vomiting,pruritus and urinary retention were recorded within 48 h after operation.The patient' s satisfaction was assessed.The maximal hip flexion and abduction ranges of motion were recorded at 12,24,36 and 48 h after operation.The times of sleep interruption resulted from pain during nighttime were also recorded.Results Compared with group PCIA,the VAS scores during activity,severity of nausea and vomiting,pruritus and urinary retention,and times of sleep interruption resulted from pain during nighttime were significantly decreased,and the overall satisfaction score and maximal hip flexion and abduction ranges of motion were increased in group CLPB (P ＜ 0.05).Conclusion CLPB combined with a bolus dose added at night can provide better efficacy for postoperative analgesia in patients undergoing hip arthroplasty than PCIA,with fewer complications.

Mice ; Animals ; Memory Disorders ; Cerebrospinal Fluid

Humans ; Myelin Sheath ; Astrocytes ; Phagocytosis ; Macrophages ; Ischemia

Female ; Humans ; Male ; Neuronal Plasticity ; Synapses ; Vasopressins