1.Study on the role of local immunity in the female genital tract with nongonococcal urethritis
Yan LI ; Quan-Zhong LIU ;
Chinese Journal of Infectious Diseases 2001;0(06):-
Objective To study the role of local immunity in the female genital tract with non- gonococcal urethritis(NGU).Methods Forty-four cervical secretions from patients with NGU were collected.After treatment with Azithromycin,22 cases were followed up.One month later,pathogen was eliminated in 15 patients.Another 27 un-infected people were included in our study.The level of secretory immunoglobulin A(SIgA)was detected using the automatic special protein analysor,and interferon-?(IFN-?)was quantified by enzyme-linked immunosorbent assay(ELISA).All data were analyzed with ANOVA.Results The levels of SIgA and IFN-?in NGU infected group were(34.29?15.92)mg/L and(97.13?24.00)ng/L respectively,while those of uninfected group were(47.57?19.61)mg/L and(120.71?33.78)ng/L,respectively(all P0.05).Conclusions SIgA and IFN-?secreted by genital tract mucosa may help pre- vent and resist the local NGU infection.However,the effect is limited,and is insufficient to eliminate infection completely and prevent reinfection.
3.Fabricating periodontal tissue engineering compound by gene modified gingival fibroblasts and acellular dermal matrix
Quan ZHONG ; Yanfen LI ; Fuhua YAN
Chinese Journal of Tissue Engineering Research 2015;(43):6906-6912
BACKGROUND:Previous studies have found that human platelet-derived growth factor-B (PDGF-B)-transfected gingival fibroblasts are capable of rapid proliferationin vitro, which can secrete platelet-derived growth factor BB proteins. OBJECTIVE:To explore the ability of PDGF-B-modified gingival fibroblasts in the acelular dermal matrixin vivo to form periodontal tissue engineering compound. METHODS: Gingival fibroblasts from Beagle dogs transfected with or without PDGF-B gene were implanted into the acelular dermal matrix. Cel growth on the acelular dermal matrix was observed. PDGF-B gene-transfected gingival fibroblasts/acelular dermal matrix composite (experimental group), gingival fibrobalsts/acelular dermal matrix composite (control group) and acelular dermal matrix (blank group) were implanted subcutaneously into the nude mice, respectively. At 2, 4, 8 weeks after implantation, skin tissues were taken and observed histologicaly. RESULTS AND CONCLUSION: PDGF-B gene-modified gingival fibroblasts and non-transfected gingival fibroblasts both grew and proliferated wel in the acelular dermal matrix. At 8 weeks after implantation, in the blank group, the surrounding cels largely entered into the acelular dermal matrix, but produce less new colagen fibers, and the cels only grew on the original colagen scaffold; in the control group, a great amount of colagen fibers formed, the original colagen fibers in the acelular dermal matrix were replaced by newly formed colagens, but the original colagen structure was reserved; in the experimental group, a large scale of permineralization formed, and mineralized nodes were arranged along the original colagen scaffold. These findings indicate that PDGF-B gene modified gingival fibroblasts can acquire osteoplastic abilities in the acelular dermal matrix in vivo.
4.Observation on effects of qige tongye decoction combined with chemotherapy in treating esophageal carcinoma.
Zhao-quan ZHANG ; Wen-jian DU ; Li-zhong LIU
Chinese Journal of Integrated Traditional and Western Medicine 2002;22(1):63-64
Aged
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Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
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Bleomycin
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administration & dosage
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analogs & derivatives
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Carcinoma, Squamous Cell
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drug therapy
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Cisplatin
;
administration & dosage
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Combined Modality Therapy
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Drugs, Chinese Herbal
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therapeutic use
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Esophageal Neoplasms
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drug therapy
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Female
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Humans
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Male
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Middle Aged
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Mitomycin
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administration & dosage
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Phytotherapy
5.Application of cystostomy drainage make thoracic cavity close drainage on pneumoconiosis.
Zhong-Quan TANG ; He-Lin LI ; Jin-Fen LIN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(4):315-316
Adult
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Aged
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Cystostomy
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Drainage
;
methods
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Humans
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Male
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Middle Aged
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Pneumothorax
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complications
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therapy
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Retrospective Studies
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Silicosis
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complications
;
therapy
6.Clinical observation of biopsy by double paths for higher positive percentage.
Hong-Quan WEI ; Gang YU ; Xiao-Tian LI ; Zhong REN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(1):67-68
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Biopsy
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methods
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Child
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Child, Preschool
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Endoscopy
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Female
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Humans
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Larynx
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pathology
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Male
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Middle Aged
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Nasopharynx
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pathology
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Young Adult
9.Analysis of photosynthetic characteristics and its influencing factors of medicinal plant Mirabilis himalaica.
Qi-Qiang GUO ; Hong QUAN ; Xiao-Zhong LAN ; Lian-Qiang LI ; Hui-E LI
China Journal of Chinese Materia Medica 2014;39(14):2769-2773
To study photosynthetic characteristics and its influencing factors in leaves of medicinal plant Mirabilis himalaica, and provide an evidence for guiding artificial planting and improving the quantity. The light-response and diurnal photosynthesis course of leaves at the booting stages of 1-3 year old M. himalaica were measured with LI-6400 system. The Results showed that the light response curves were fitted well by non rectangle hyperbola equation (R2 > or = 0.98). The values of the maximum photosynthetic rate (Pmax) and light use efficiency of three-year old M. himalaica leaves were higher than those of 1-2 year old individuals. The diurnal variation of net photosynthetic rate (Pn) and stomatal conductance (Gs) of 2-3 year old M. himalaica were typical double-peak curves determinately regulated by stomatal conductance. However, transpiration rate (Tr) of 1-3 year old plants leaves were single-peak curve, which was self-protection of harm reduction caused by the higher temperature at noontime. Correlation analysis showed that the changes between photosynthetic active radiation (PFD), air temperature (T ) and Pn, were significant positive related. Therefore, M. himalaica is a typical sun plant, which should be planted under the sufficient sunshine field and prolong the growing ages suitably in order to improve the yield.
Breeding
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Mirabilis
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growth & development
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metabolism
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Models, Biological
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Photosynthesis
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Temperature
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Time Factors
10.Effect of respiratory movement on cone beam computed tomography images.
Chang GUO ; Renming ZHONG ; Guangjun LI ; Chuanxian JI ; Chengqiang LI ; Hong QUAN ; Sen BAI
Journal of Biomedical Engineering 2014;31(2):314-318
To investigate the impact of respiratory movement to determine the target volume on cone beam CT (CBCT) for lung tumor, we used CIRS dynamic thorax phantom (Model-CIRS008) to simulate the sinusoidal motion of lung tumor. With a constant amplitude, the ratio of the time of near-end-expiratory and near-end-inspiratory (E/I) changed when it was scanned with CBCT. We analyzed the contrast changes of target by extracting the CT value of each pixel on the center line of the target movement direction. The targets were contoured with region growing method and compared with the motion volume generated by the tumor trajectory method. The result showed that the contrast of near-end-expiratory increased and the contrast of near-end-inspiratory decreased with increasing E/I. The contoured volume generated by region growing method decreased with increasing E/I. When E/I = 4, the amplitude A = 1 cm, diameter of 1 cm and 3 cm target volumes were reduced by 48.2% and 22.7%. The study showed that CBCT was not suitable to be used to accurately determine the range of lung tumor movement. The internal target volume (ITV) may be underestimated in CBCT images.
Cone-Beam Computed Tomography
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Humans
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Lung Neoplasms
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diagnostic imaging
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Movement
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Phantoms, Imaging
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Respiration