1.Survey of knowledge, attitude and practice of malaria among villagers in rural areas of Yunnan Province
Sheng ZHOU ; Quan LU ; Longfei DU ; Mingdong YANG ; Li LI ; Rui YANG ; Zhonghua YANG
Chinese Journal of Schistosomiasis Control 2010;22(1):87-89
Objective To understand the status of knowledge,attitude and behavior on malaria of residents in endemic areas of Yunnan Province.so as to provide evidences for implementation of Global Fund MaIaIia Control Program.Methods The subjects were selected by the method of multi-level sampling in 47 countries and were investigated by questionnaire.Results A toatl of 40 940 households in 883 natural villages were investigated.39 234 questionnaires were effective with an effective rate of 95.83%.A total of 60.32%interviewees knew malaria symptom and signs,22.41%of them knew the route of transmission,17.65% knew the preventive measures.57.61% had the willing to use insecticide-treated bed nets(ITNs),and 8.68%of the households owned ITNs.Conclusion Health edueion and promotion regarding critical malria interventions should be strengthened in the following implementation.
2.Construction and analysis of root cDNA library in Glycyrrhiza uralensis.
Quan YANG ; Hui ZHANG ; Wen-quan WANG ; Chun-sheng LIU ; Sheng-li WEI
China Journal of Chinese Materia Medica 2008;33(12):1386-1389
OBJECTIVETo screen and isolate secondary metabolite biosynthesis-related gene for establishing the foundation of functional gene research, we construct a cDNA library of Glycyrrhiza uralensis.
METHODTotal RNA was isolated from G. uralensis using the method of lithium chloride sedimentation. Double strand cDNA was joined into pBlueScript II vector. The number of clones, recombinant rate and length of insert fragments were determined.
RESULTThe capacity of the original library was 1.15 x 10(7) with a recombinant rate of 98.2% and the inserted cDNA fragments ranged from 0.5 to 4.8 kb. 126 ESTs through random sequencing were obtained. The most homological proteins came from leguminous plants, including Arabidopsis thaliana, Oryza sativa, and so on. Most of the proteins were related to genes linking cell matabolism, resistance, growth retardation and dormancy.
CONCLUSIONThe library has enough capacity, high recombinant rate and long insert fragment for the further study.
Computational Biology ; DNA, Recombinant ; genetics ; Electrophoresis, Agar Gel ; Expressed Sequence Tags ; Gene Library ; Glycyrrhiza uralensis ; genetics ; RNA, Messenger ; analysis ; genetics ; RNA, Plant ; analysis ; genetics ; Sequence Analysis, DNA
3.The deacclimatization symptom scores of 159 youth returning to the same plain from different plateau.
Yin-Hu WANG ; Yan WANG ; Sheng-Hong YANG ; Bin LI ; Qi-Quan ZHOU
Chinese Journal of Applied Physiology 2014;30(1):18-21
OBJECTIVETo analysis deacclimatization symptom scores of 159 youth from Karakoram and Tibet Ali area, and provide the basis for the development of relevant prevention and control measures.
METHODSUsing the method of epidemiological symptoms questionnaire, 18 symptoms of 190 youth who returned to the plain area from the different plateau were investigated. The symptom scores of different altitude, age, the time of staying, different units, continuous or intermittent stage and education were surveyed.
RESULTSDeacclimatization symptom scores among 5,000 meter groups were significantly higher than those of 4,300 meter and 3,700 meter group (P < 0.05, P < 0.01). There was no significant difference between the 4,300 meter group and the 3,700 meter group (P > 0.05). There were significant differences among the stayed personnel (different age, position, unit, education, time, continuous or intermittent) (P < 0.01). There was significant difference between the continuous defended the group and intermittent group (P < 0.01).
CONCLUSIONDeacclimatization symptom scores were related to the plateau exposure time, altitude, workload, plateau continued exposure. The older, the longer exposure, the higher altitude, the greater workload at plateau were showed higher deacclimatization symptom score.
Acclimatization ; Altitude ; Altitude Sickness ; physiopathology ; Humans ; Surveys and Questionnaires ; Tibet
4.Study on resource and varieties of Guoshangye.
Xian-You QU ; Shong-Yun QIN ; De-Quan YANG ; Quan-Sen LI ; Fang-Sheng PENG
China Journal of Chinese Materia Medica 2006;31(2):110-114
OBJECTIVETo investigate the varieties, resoures and identification authentication of Guoshangye used in Tuja and Miao's folk.
METHODThrough field investigations and comparing the collected specimens and literatures, the classification and identification of the species were studied.
RESULT AND CONCLUSIONOrigin of Guoshangye plant was from Pholidota yunnanensis, Bulbophyllum andersonii, B. odoratissimum, B. kamgtimgemse, ect. their morphological characters were identified. Due to limited resource of Guoshangye, the development and protection should be paid more attention.
Analgesics ; pharmacology ; Animals ; Antitussive Agents ; pharmacology ; Conservation of Natural Resources ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; History, 19th Century ; History, Medieval ; Humans ; Orchidaceae ; anatomy & histology ; chemistry ; classification ; Pharmacognosy ; history ; Plant Roots ; anatomy & histology ; chemistry ; Plants, Medicinal ; anatomy & histology ; chemistry ; classification
5.The feasibility of application of reverse docking method to the selectivity studies of protein kinase inhibitors.
Ting-Lin LIU ; Huan-Zhang XIE ; Yu-Quan WEI ; Sheng-Yong YANG
Acta Pharmaceutica Sinica 2009;44(7):758-763
This investigation is to explore the feasibility of applying reverse docking method to the selectivity studies of protein kinase inhibitors. Firstly, a database that consists of 422 protein kinase structures was established through collecting the reported crystal structures or homology modeling. Then a reverse docking based method of protein kinase target screening was established, followed by the optimization of related parameters and scoring functions. Finally, seven typical selective kinase inhibitors were used to test the established method. The results show that the selective targets of these inhibitors have relatively high scoring function values (ranking in the first 35% of the tested kinase targets according to the scoring function values). This implies that the reverse docking method can be applied to the virtual screening of kinase targets and further to the selectivity studies of protein kinase inhibitors.
Alternative Splicing
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Drug Delivery Systems
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Drug Evaluation, Preclinical
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methods
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Gene Targeting
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Models, Molecular
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Protein Binding
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Protein Kinase Inhibitors
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chemistry
6.A quantitative study on ultrasonographic texture analysis of injured porcine striped muscle induced by thermal and chemical factors
Jia-qi, ZHAO ; Jian-quan, ZHANG ; Qi, XU ; Yan-qiu, CHEN ; Jian-guo, SHENG ; Feng, LU ; Tian, YANG
Chinese Journal of Medical Ultrasound (Electronic Edition) 2013;(8):674-680
Objective To quantitatively analyze and compare the texture features of thermal and chemical lesions on the porcine striated muscle, in vitro extracted from high-frequency ultrasonograms using computer-assisted image analysis technique, and to investigate the application values. Methods The thermal lesion and chemical lesion were induced in vitro in porcine striated muscle by microwave ablation and anhydrous acetic acid injection, respectively. The two dimension (2D) ultrasonographic ifndings were qualitatively compared between the groups of thermal and chemical lesion models, in which eight textural features in geometric mathematics extracted from 2D ultrasonograms were quantitatively analyzed by a technique of computer-assisted image analysis named multiscale decomposition method of echo intensity of interface relfections. Results As expected, microwave ablation and anhydrous acetic acid caused signiifcant changes of several texture features extracted from ultrasonograms. There were significant differences between the normal group and microwave ablation group in grayscale mean (Mean), irregularity (IRGL) and periodicity of distribution (POD) as follows (Mean: 1.9143±0.2914 vs 1.2334±0.3357, t=-5.306, P=0.000; IRGL: 0.5577±0.0334 vs 0.5092±0.0459, t=-2.957, P=0.007; POD: 0.000 27±0.000 005 vs 0.000 29±0.000 008, t=4.782, P=0.000). There were signiifcant differences between the normal group and anhydrous acetic acid injection group in number of blobs (NOB), size of blobs (SOB) and periodicity of distribution (POD) as follows (NOB: 51.0324±13.6998 vs 31.6042±4.8315, t=4.633, P=0.000; SOB:16.4843±3.9349 vs 25.6230±2.3555, t=6.903, P=0.000;POD:0.000 26±0.000 015 vs 0.000 29±0.000 008, t=-4.459, P=0.000). For each group of injured regions, there were significant differences between the microwave ablation group and anhydrous acetic acid injection group in Mean, IRGL, NOB and SOB as follows (Mean: 1.2664±0.2688 vs 1.9143±0.2914, t=-5.661, P=0.000; IRGL: 0.5220±0.0422 vs 0.5577±0.0334, t=-2.295, P=0.032;NOB:51.0324±13.6998 vs 34.5856±2.6362, t=4.048, P=0.000;SOB:16.4843±3.9349 vs 25.3176±2.3501, t=-6.676, P=0.000). Conclusion Technique of computer-assisted image analysis named multiscale decomposition method of echo intensity of interface relfections, based on multiscale blob features extraction, was useful to differentiate ultrasonic texture features between the groups injured in our study, which established quantitative muscle ultrasound as a practical and reliable tool for the muscle injury diagnosis to distinguish the structural changes induced by different physiochemical factors.
7.Therapeutic intervention against deacclimatization to high altitude.
Yin-Hu WANG ; Qi-Quan ZHOU ; Sheng-Hong YANG ; Yan WANG ; Bin LI ; Chao-Liang LONG ; Hai WANG
Chinese Journal of Applied Physiology 2013;29(6):512-517
The incidence of deacclimatization to high altitude syndrome (DAHAS) prevailed up to 80% in highland troops, and 100% in manual workers, and severe DAHAS could significantly affects patients' health, work and life. So it is imperative to develop effective prevention and treatment measures for DAHAS. The present review analyzes effective prophylactic and therapeutic measures against DAHAS, implemented at our hospital.
Acclimatization
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Altitude
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Altitude Sickness
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prevention & control
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therapy
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Humans
8.Construction of efficient conjugal plasmids between Escherichia coli and Streptomycetes.
Hong-Bo MO ; Lin-Quan BAI ; Sheng-Lan WANG ; Ke-Qian YANG
Chinese Journal of Biotechnology 2004;20(5):662-666
Conjugal plasmid pGH112 has been developed based on the replicons of Streptomyces coelicolor plasmid SCP2 and E. coli ColE. The plasmid contains ampicilin resistance gene(amp) for selection in E. coli and thiostrepton resistance gene (tsr) for selection in Streptomycetes, and a 0.76 kb oriT fragment of (IncP) RK2. Conjugal transfer of pGH112 was performed from E. coli to S. coelicolor A3(2), S. avermitilis, S. lividans TK54, S. toxytricini NNRL15443, S. venezuelae ISP5230 and Sacc. erythraea by conjugation, results show that the plasmid was able to transfer efficenctly from E. coli to Streptomycetes, was stably inherited in the recipients. pGH113 was constructed from pGH112 by combining the constitutive ermE promoter with green fluorescent protein gene(gfp).
Ampicillin Resistance
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genetics
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Conjugation, Genetic
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Escherichia coli
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genetics
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Green Fluorescent Proteins
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genetics
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Plasmids
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Streptomycetaceae
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genetics
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Thiostrepton
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pharmacology
9.Optimized methods for biofilm analysis in Yersinia pestis.
Nan FANG ; He GAO ; Li WANG ; Shi QU ; Yi Quan ZHANG ; Rui Fu YANG ; Dong Sheng ZHOU
Biomedical and Environmental Sciences 2013;26(5):408-411
10.Effect of graded composite zirconia-hydroxyapatite on viability of rat osteoblast cells cultured in vitro
Ren-Fu QUAN ; Di-Sheng YANG ; Xu-Dong MIAO ; Wei LI ; Xiao-Chun WU ; Hong-Bin WANG
Chinese Journal of Trauma 2003;0(11):-
Objective To evaluate the effect of a novel orthopedic biomaterial,graded composite zireonia(ZrO2)hydroxyapatite(HAP)on activity of rat osteoblast ceils(OB)cultured in vitro. Methods The pure zirconia material was used as control to measure surface roughness of the composite material that was examined by using scanning electron microscope(SEM)and X-ray diffraetometer (XRD).The rat osteoblast cells were cultured on the two materials.Alkali phosphatase(ALP)of the two groups was measured and ELISA was used to detect IL-6 and TGF-?eoncentration of the supematant of OB cells.Tumor growth factor-?(TGF-?)mRNA was detected by RT-PCR.SEM was used to observe OB cells on the two materials.The extract of the composite material was used for a eytotoxicity test to cal- culate the relative proliferation rate(RGR)and classify the toxicity.Results The surface roughness of the gradual composite materials was significantly higher than that of the control materials(P<0.01). The ALP of the gradual material group was markedly higher than that of the control group at different in- tervals.There was significant difference of the IL-6 and TGF-?concentrations 2-4 days after culture be- tween two groups(P<0.05,P<0.01).The mRNA level of TGF-?of the two OB groups also showed marked statistical difference(P<0.01).The ossification of the OB cells on the composed material was marked after 14 days.The MTT color experiments showed no statistic significance between materials group and negative group,with the toxicity at levelⅠand 0(P<0.05).Conclusion Graded composite ZrO2 HAP can significantly promote proliferation and differentiation of OB cells cultured in vitro and has good biocompatibility.