Objective To investigate the clinical effect of creatinine phosphate sodium combined with Xinjikang Granules in treating children with viral myocarditis ,and provide reference for clinical treatment .Methods 160 children patients with viral myo‐carditis from January 2011 to January 2015 were selected and randomly divided into the control group and the observation group ac‐cording to the digital table method ,80 cases in each group .The control group used the symptomatic treatment of fructose diphos‐phate (FDP) ,vitamin C ,coenzyme Q10 and ribavirin ,while the observation group used creatine phosphate sodium and Xinjikang Granules on the basis of control group .The clinical effects ,ECG improvement ,cardiac enzymes and troponin I levels were compared between the two groups .Results The effective rate in the observation group was 87 .50% ,which was significantly higher than 70 .00% in the control group ,the difference was statistically significant (χ2 =7 .320 ,P=0 .004);the effective rate of ECG improve‐ment in the observation group was 90 .00% ,which was significantly higher than 75 .00% in the control group ,the difference was statistically significant (χ2 = 6 .234 ,P=0 .005);the levels of AST ,CPK ,LDH ,CK‐MB ,HBDH and cTnI after treatment in the two groups were significantly improved compared with before treatment ,the differences were statistically significant (P< 0 .05) ,the levels of AST ,CPK ,LDH ,CK‐MB ,HBDH ,and cTnI after treatment in the observation group were (29 .98 ± 4 .66)U/L ,(184 .41 ± 5 .97)U/L ,(314 .25 ± 9 .84)U/L ,(22 .29 ± 2 .98)U/L ,(268 .37 ± 8 .64)U/L and (0 .13 ± 0 .04)μg/L respectively ,which showed the statistical differences compared with the control group ,[(33 .49 ± 4 .98)U/L、(196 .49 ± 6 .71)U/L、(328 .64 ± 11 .14)U/L、(26 .53 ± 3 .44)U/L、(298 .68 ± 10 .64)U/L、(0 .25 ± 0 .05)μg/L ,P<0 .05] .Conclusion Creatinine phosphate sodium in treating children with viral myocarditis has significant clinical effect ,and is worthy of clinical promotion and application .

A series of medical equipment question is introduced including currency use, combination, digital function and whole efficiency. By using system theory, the thought and viewpoint were put forward that how to solve equipment classification, "three turn" design (generalization, modularization, serialization), informationization alteration and affixation etc. Some thoughs on development strategy of medical equipment and creative information condition of field battle can be referred and provided.

Objective To explore in vitro effects and the mechanism for FLAG regimen compared with IA regimen in P-glycoprotein-positive and -negative acute myeloblastic leukemia(AML) cell lines. Methods The expression of P-glycoprotein in K562 and K562/A02 cells were analyzed by flow cytometry. The effects of FLAG and IA on the proliferation of K562 and K562/A02 cells were detected by MTT assay. The Ara-CTP and Ara-C levels in those cells were measured by HPLC,the gene expression of hENT1 in K562 and K562/A02 cells was detected by real-time PCR. Results The positive rates of P- glycoprotein were (1.32±0.24)% in K562 cell and (97.66±3.77)% in K562/A02 cell, respectively. The expression of P- glycoprotein had no change after treated with FLAG or IA. The cytotoxicity to K562 of IA was better than FLAG [(84.41±9.33) % v.s (73.17±13.20)%, P<0.05], and the cytotoxicity to K562/A02 of FLAG was better than IA [(70.55±11.32)% v.s (48.46±12.81)%, P<0.01]. Ara-C and Ara-CTP accumulation and hENT1 expression in AML cells treated with FLAG were higher than that treated with IA. Conclusion P-glycoprotein-positive AML cells are more sensitive to FLAG regimen than IA regimen. The biochemical modulation of Ara-CTP and Ara-C may be the major mechanism.

Objective To evaluate the effectiveness and safety of total pelvic floor reconstruction treating female pelvic organ prolapse with prolift system .Methods The effectiveness and complications 24 cases genital pro-lapse according to POP Q stages who underwent total pelvic floor reconstruction surgery with prolift system were retro -spectively reviewed.Results The mean operative time was (97.5 ±23.3) min.The average blood loss (68.5 ± 28.1)mL.The mean postoperative hospital′s day was (8.9 ±2.3)days.The symptoms of all patients with peivic floor prolapse disappeared .2 cases sufferd pain and mesh erosion ,1 case had buttock abscess during follow-up.All recov-ered after treatment .Conclusion The short-term curative of total pelvic floor reconstruction surgery with prolift sys-tem is good.These cases have some postoperative complication .Such operation mode should be selected comprehen-sively.

Anemia, Hemolytic ; chemically induced ; therapy ; Female ; Humans ; Paraquat ; poisoning ; Poisoning ; therapy ; Young Adult

This article introduces the genes related to pathogenesis and prognosis of myeloproliferative neoplasms (MPN) and the current situation of MPN domestic and overseas,which have been reported in the 56th ASH annual meeting.It is emphasized on essential evaluation of the risk score using IPSET in ET,Tefferi in PV and DIPSS-plus in PMF separately.It has assessed advancements in allogeneic hematopoietic stem cell transplantation,phlebotomy,cytoreductive therapy and drug therapy.The article pays more attention to aspirin,anagrelide,JAK2 targeted agent ruxolitinib and thalidomide which is domestically used in the treatment of PMF.

Adult ; Diagnosis, Differential ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Laryngeal Neoplasms ; diagnostic imaging ; metabolism ; pathology ; surgery ; Laryngectomy ; Leiomyosarcoma ; pathology ; Male ; Rhabdomyosarcoma ; diagnostic imaging ; metabolism ; pathology ; surgery ; Tomography, X-Ray Computed ; Vimentin ; metabolism

Objective To evaluate the safety and accuracy of CT-guided cutting needle biopsy for the hepatic lesions near diaphragmatic dome.Methods A total of 25 cases with hepatic lesions near the diaphragmatic dome were undertaken CT-guided cutting needle biopsy using 16 gauge or 18 gauge core biopsy needles.Results Histological examination showed malignancy in 17 cases and benign in 8 with 2 false negative results(8%),and there were no false positive results.The specificities of malignant and benign lesions were 100% and 75%,respectively.Overall accuracy was 92%.Pneumothorax,needle tract hemorrhage,and subcapsular hepatic hemorrhage occurred in 2(8%),1(4%)and 1(4%),respectively.Conclusion CT-guided cutting needle biopsy for the hepatic lesions near diaphragmatic dome is a reliable and relatively safe diagnostic method.(J Intervent Radiol,2007,16:838-840)

Cancer, a group of various diseases involving unregulated cell growth, still imposes a global threat to public health. Ginseng plants, which contains a wide variety of saponins, known as ginsenosides, is an age-old remedy for human ailments, including cancer.This review sheds light on the molecular mechanisms underlying the cancer chemopreventive and/or chemotherapeutic activity of ginsenosides and their metabolites with particular focus on the modulation of cell signaling pathways associated with cell proliferation, apoptosis, angiogenesis, oxidative stress, inflammation and the metastasis of cancer cells.The review could enhance the understanding of the anticancer mechanisms of ginsenosides, provide directions for clinical practice and give more information for developing novel an-ticancer agents with high efficiency, low toxicity and weak resistance.

BACKGROUND: Host endothelial cells are important targets of alloreactive donor cytotoxic T lymphocytes in graft-versus-host disease (GVHD). And the expression of tissue factor in vascular endothelial cells is up-regulated post-injury and activation. So tissue factor expression in vascular endothelial cells may play a pivotal role in the pathogenesis of GVHD.OBJECTIVE: To investigate the molecular mechanism of allogeneic T cell-induced tissue factor (TF) expression in vascular endothelial cells.DESIGN, TIME AND SETTING: The cytology observation was performed at immunological laboratory of ongji Medical College, Huazhong University of Science and Technology from March to October 2008.MATERIALS: Human primary umbilical vein endothelial cells (HUVECs) were purchased from Science cell laboratory (CA, USA).METHODS: Allogeneic CD4~+ and CD8~+T cells were isolated from peripheral blood mononuclear cells (PBMC) by positive selection using magnetic beads coated with anti-CD4 or anti-CD8 antibody. After thorough washing with PBS, 1×10~6 allogeneic CD4~+T cells or CD8~+T cells were added to HUVECs (ratio 10:1). The cells were grown at 37 ℃ in a humidified atmosphere containing c-jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated protein kinase (ERK). HUVECs were pretreated with TF antibody 1 hour before co-culturing; the untreated HUVECs were used as controls. MAIN OUTCOME MEASURES: Allogeneic T cells-induced TF expression in vascular endothelial cells was determined by real-time PCR and flow cytometry. Western blot was used to examine allogeneic T cells-induced phosphorylation of MAPK in HUVEC.RESULTS: After co-culturing with allogeneic CD4~+T cells or CD8~+T cells for 6, 12, and 24 hours, the expression rates of TF on HUVEC membrane were obvious increased than that of the control group (P < 0.05), the expression levels of TF mRNA in HUVEC were also significantly elevated (P < 0.05). Allogeneic CD4~+T cells-induced expression rates of TF were significantly higher than allogeneic CD8~+T cells-induced expression rates at 12 and 24 hours after culture. The expression of p38MAPK and JNK was enhanced after culture with allogeneic T cells, yet the ERK expression had no changes. The p38MAPK or JNK can notably down regulated TF expression, which could block the TF expression when worked together. CONCLUSION: Allogeneic T cells induced TF expression in HUVECs via activation of p38MAPK and JNK.