2.Clinical comparative study on conventional surgical and subfascial endoscopic perforator surgery in great venous varicosity
Hai LIN ; Jun DING ; E-Zhang LIU ; Zhen-Quan WEI ; Xian-Ming CHEN ; Zhong-Jiang LU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To compare the curative effect of high ligation+exfoliation and subfascial endoscopic perforator surgery(SEPS)for superficial varicose veins in calf+invagination spot-striping surgery in great venous varicosity.Methods Study group(42 patients)accepted SEPS+invagination spot-striping surgery and control group (42 patients)accepted traditional surgeries.Operation duration,bleeding volume in operation,the time of beginning movement away from bed after operation,hospitalization duration,the degree of pain,the scar,the recrudescence af- ter operation and the instance of the ulcer heals of two groups were compared.Results Operation duration,bleeding volume in operation,the time of begin movement away from bed after operation and hospitalization durations of study group were significantly lower than those of control group(P0.05).All of the patients in study group recovered without severe syndromes such as venous thrombosis,skin necrosis,lower limb functional disorder etc.They had no recrudesce after 4~16 months and were satisfied with the curative effect.Con- elusions The clinical curative effect of SEPS+invagination spot-striping surgery in great venous varicosity is superi- or to that of traditional operation and it has the advantages such as minor wound,few scars,light pains,short hospi- talization duration,without recrudescence,the ulcer heals quickly and so on.
3.Application of thioflavin T staining in detection of β-amyloid.
Qiao-quan ZHANG ; Jian-quan SHI ; Jun XU ; Xin-xin CHENG ; Hai-qing ZHU ; Jun CHEN
Chinese Journal of Pathology 2013;42(11):764-765
Alzheimer Disease
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metabolism
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pathology
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Amyloid beta-Peptides
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metabolism
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Animals
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Brain
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metabolism
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pathology
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Cerebral Amyloid Angiopathy
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metabolism
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pathology
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Fluorescent Dyes
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chemistry
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Humans
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Mice
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Peptide Fragments
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metabolism
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Staining and Labeling
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Thiazoles
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chemistry
4.Effects of moxibustion at Shenque (CV8) on gastrointestinal function in rats undergoing gastric perforation repair.
Quan ZHENG ; Huaying LI ; Xiaomin WEN ; Kuncai ZHANG
Journal of Southern Medical University 2014;34(1):124-127
OBJECTIVETo assess the effects of moxibustion at Shenque (CV8) in promoting gastrointestinal function recovery in rats following gastric perforation repair and explore the underlying mechanism.
METHODSThirty male SD rats with glacial acetic acid-induced gastric perforation underwent surgical repair of the perforation. The rats were then randomized 8 days later into model group (n=10), domperidone group (n=10), and moxibustion group(n=10) and treated with physiologic saline, domperidone suspension, and moxibustion at Shenque (CV8), respectively. Gastric antral myoelectric activities of the rats were observed and peripheral blood levels of TNF-α, IL-6, and T lymphocyte subpopulation were determined.
RESULTSGastric antral myoelectric activities in rats receiving moxibustion were stronger than those in the model group (P<0.05) but comparable with those in domperidone group (P>0.05). TNF-α and IL-6 level were decreased significantly and T lymphocyte subpopulations increased significantly in moxibustion group compared with those in the model and domperidone groups (P<0.05).
CONCLUSIONMoxibustion at Shenque (CV8) can promote the recovery of gastrointestinal functions in rats undergoing gastric perforation repair possibly by enhancing gastrointestinal electric activity, suppressing inflammation, and improving the cellular immune function, and can therefore serve as a simple and effective adjuvant therapy during the perioperative period.
Acupuncture Points ; Animals ; Interleukin-6 ; blood ; Male ; Moxibustion ; Postoperative Period ; Rats ; Rats, Sprague-Dawley ; Recovery of Function ; Stomach Rupture ; therapy ; T-Lymphocyte Subsets ; cytology ; Tumor Necrosis Factor-alpha ; blood
5.Comparison of postoperative drainage and systemic trauma response after endoscopic and traditional near total thyroidectomy.
Yong-Quan ZHANG ; Zhi-Chao LI ; Fei CHEN ; Hong-Juan WANG ; Qiang LI
Journal of Southern Medical University 2017;37(10):1364-1369
OBJECTIVETo investigate the difference of postoperative drainage and systemic trauma response between endoscopic and traditional near total thyroidectomy to provide the basis for selecting appropriate operative methods.
METHODSIn this prospective clinical controlled study, 80 patientsscheduled for near total bilateral thyroidectomy for the first time were divided equally into endoscopic surgery group (group A) and open surgery group (group B). The total drainage volume after operation, postoperative extubation time, and postoperative daily drainage volume were recorded after the operation. The contents of triglyceride (TG) and total protein (TP) were determined in the postoperative drainage fluid onthe first day. The levels of interleukin 6 (IL6), high sensitive C reactive protein (HSCRP), alpha 1 acid glycoprotein (AAG), ceruloplasmin (CER) and haptoglobin (HPT) in venous blood were tested before the operation and on the first day after surgery.
RESULTSCompared with those in group B, the postoperative drainage volumein group Aincreased significantly (P=0.000) and the postoperative extubation time was significantly prolonged (P=0.000); the mean postoperative daily drainage volume was significantly larger ingroup A than in group B (P=0.000) and tended to decrease with time in both groups. There was no significant difference in the content of triglycerideortotal protein in the drainage fluid between the two groups on the first day after operation (P=0.429 and 0.324, respectively). In both groups, the contents of AAG, ceruloplasmin and haptoglobin on the first postoperative day were all similar with those measurement before operation (P>0.05), but significant variations occurred in the levels of IL6 and HSCRP on the first postoperative day (P=0.000). The serum levels of IL?6 or HS?CRP did not differ significantly between the two groups on the first day after operation (P=0.054 and 0.066, respectively).
CONCLUSIONCompared with open surgery, endoscopic near total bilateral thyroidectomyis associated with an increased the volume of postoperative drainage and a prolonged time of extubationbut not an increased systemic trauma response. Therefore, endoscopic surgery can serve as one of the routine options for patients who are concerned with neckscars resulting from open surgeries.
6.Effect of dexamethasone contamination in drinking water on intestinal flora in mice.
Xi YANG ; Xiao-Yu LI ; Dan SI ; Zhi-Bang YANG ; Zhong-Yuan HE ; Nan-Chen ZHANG ; Shan-Shan ZHANG ; Zhong-Quan SHI
Journal of Southern Medical University 2016;36(2):238-243
OBJECTIVETo evaluate the effect of water pollution with dexamethasone on intestinal flora in mice.
METHODSTwenty Balb/c mice were randomly divided into control group and low-, moderate- and high-dose dexamethasone groups. The mice in dexamethasone groups were exposed to dexamethasone sodium phosphate in drinking water at doses of 0.035, 0.225, and 2.25 ng for 36 days. The changes in behaviors, fur condition, and feces of the mice were observed daily. All the mice were sacrificed at 36 days and the tissues in the ileocecal region was collected for denaturant gradient gel electrophoresis (DGGE) of 16S rDNA V6 variable regions of microbes and sequence analysis with BLAST.
RESULTSThe mice in the 3 dexamethasone groups all showed aggressive behaviors. Cluster analysis of DGGE graph showed relatively stable floras in the ileocecal region in all the mice, but principal component analysis identified differences in the dominating flora among the groups. Diversity analysis of the flora revealed significantly increased amount and types of bacteria in the intestinal flora in all the 3 dexamethasone groups (P<0.05 or 0.01) compared with the control group. Sequence analysis of 16S rDNA V6 regions showed 15 common bacterial species and 2 differential species between the dexamethasone groups and the control group with changes in the type and proportion of the dominating bacterium in the dexamethasone groups. Lactobacillus colonization was detected in the control group but not in moderate- and high-dose dexamethasone groups, and Shigella species were found in the latter two groups.
CONCLUSIONSWater contamination with dexamethasone can affect the nervous system of mice, cause changes in the types and amounts of intestinal bacteria and the dominating bacteria, and inhibit the colonization of probiotics in the intestinal floras to increase the risk of invasion by intestinal pathogenic bacteria.
Animals ; Bacteria ; classification ; Dexamethasone ; pharmacology ; Drinking Water ; chemistry ; Feces ; Gastrointestinal Microbiome ; drug effects ; Lactobacillus ; isolation & purification ; Mice ; Mice, Inbred BALB C ; Probiotics ; RNA, Bacterial ; genetics ; RNA, Ribosomal, 16S ; genetics ; Shigella ; isolation & purification
7.The molecular mechanisms of As_2O_3- induced un-terminal differentiation of HL-60 cells
hou-qiao, BAI ; tie-jun, GUO ; pei-e, WEN ; hai-quan, REN ; yu-kun, ZHANG ; tian-hua, TANG ; hao, ZHANG ; gao-feng, BI ; jia-ning, LIU ; guo-sheng, JIANG
Journal of International Oncology 2006;0(05):-
To approach the effect of CCAAT/enhancer binding proteins (C/EBPs) on un-terminal differentiation of HL-60 cells after treatment with Arsenic Trioxide ( As_2O_3) . Methods The changes of cell morphology were observed by Wright staining, the alteration in the cell proliferation was determined by WST1 experiment and the NBT reduction assay was used to detect the differentiation condition of cells, determination and analysis cell cycle. The expressions of C/EBP? and C/EBP? mRNA in HL-60 cells exposed to ATRA and As_2O_3 were assayed by semi-quantitative RT-PCR. Results It was found that ATRA could up- regulate the mRNA expression of C/EBP? obviously, but down-regulate the mRNA expression of C/EBP?. As_2O_3 could up-regulate the mRNA expression of C/EBP? lightly, down-regulate the expression of C/EBP?. Conclusion Both of ATRA and As_2O_3 can down-regulate the mRNA expression of C/EBP?,but there is no significant difference between these two groups,ATRA and As2O3 can up- regulate the mRNA expression of C/EBP?, with significant differences (P
8.Efficient reprogramming of human cord blood CD34(+) cells for formation of induced pluripotent stem cells with non-integrating plasmid system.
Quan-E ZHANG ; Shu-Ping LIU ; Yan-Feng LIU ; Hong-Yan ZHANG ; Wei-Ping YUAN ; Gui-Bin CHEN ; Yan-Xin LI ; Jing XU
Journal of Experimental Hematology 2013;21(3):728-734
This study was to establish the episomal vector reprogramming method to reprogram iPSC from human cord blood (CB) CD34(+) cells. The non-integrating plasmids of pEB-C5 and pEB-Tg were transfected into short-term cultured CB CD34(+) cells by using the nucleofector, so as to demonstrate efficient reprogramming of CB CD34(+) cells. Within 14 days of one-time transfection by two plasmids together, up to 200 iPSC-like colonies per 2 million transfected CB CD34(+) cells were generated. The results showed that the pluripotency of iPSC-derived CB CD34(+) cells was similar to that of hESC and the karyotypes of iPSC were normal. In addition, no vector integration was found in iPSC of 9th and 10th passages. Furthermore, hiPSC formed teratoma with three embryonic germ layers. It is concluded that the integration-free method to generate human iPSC from CB CD34(+) cells is reliable and can provide new ways for both research and future clinical applications.
Animals
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Antigens, CD34
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immunology
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Cell Culture Techniques
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Cells, Cultured
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Cellular Reprogramming
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Fetal Blood
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cytology
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immunology
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Fibroblasts
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cytology
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Humans
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Induced Pluripotent Stem Cells
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cytology
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Mice
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Plasmids
9.Role of chloride channels in gambogic acid-induced apoptosis of poorly differentiated nasopharyngeal carcinoma cells.
Zhi-quan BAI ; Hua-rong LI ; Hai-feng ZHANG ; Shan-wen LIU ; Lin-yan ZHU ; Wen-cai YE ; Li-xin CHEN ; Li-wei WANG
Journal of Southern Medical University 2011;31(8):1304-1308
OBJECTIVETo investigate the role of chloride channels in the apoptosis of poorly differentiated nasopharyngeal carcinoma CNE-2Z cells induced by gambogic acid (GA).
METHODSMTT assay was applied to detect the proliferation of CNE-2Z cells after GA treatment, and the cell apoptosis was detected by Hoechst 33342 staining. Whole-cell patch clamp technique was employed to record GA-activated Cl(-) currents in the cells.
RESULTSGA inhibited the cell proliferation in a time- and concentration-dependent manner with an IC(50) of 3.1 µmol/L for a 48-h treatment. The apoptosis-inducing effect of 8 µmol/L GA was attenuated by the chloride channel blocker NPPB (100 µmol/L) and tamoxifen (20 µmol/L). GA induced an outward-rectified Cl(-) current in the cells, which was significantly inhibited by NPPB.
CONCLUSIONGA suppresses cell proliferation and induces apoptosis by activating Cl(-) channels in CNE-2Z cells, suggesting the important role of Cl(-) channels in GA-induced apoptosis.
Apoptosis ; drug effects ; Cell Line, Tumor ; Chloride Channels ; drug effects ; physiology ; Humans ; Nasopharyngeal Neoplasms ; pathology ; Patch-Clamp Techniques ; Xanthones ; pharmacology
10.Interferon-induced transmembrane protein 3 knock-down inhibits proliferation and invasion of hepatocellular carcinoma HepG2 cells in vitro.
Rong-Shou WU ; Lin-Quan WU ; Ke-Hao LI ; En-Liang LI ; Qian FENG ; Jing-Ling ZHANG
Journal of Southern Medical University 2016;36(2):244-249
OBJECTIVETo investigate the abnormal expression of interferon-induced transmembrane protein 3 (IFITM3) in hepatocellular carcinoma (HCC) and the effect of IFITM3 knock-down on the biological behaviors of hepatocellular carcinoma HepG2 cells.
METHODSWestern blot analysis and immunohistochemical staining were used to determine the expression of IFITM3 protein in 60 HCC samples and paired adjacent tissues. A small interfering RNA fragments of IFITM3 (IFITM3 siRNA) was transiently transfected into HepG2 cells and expressions of IFITM3 at mRNA and protein levels were examined by qRT-PCR and Western blotting. The changes in the proliferation of the transfected cells were determined using cell counting kit 8 (CCK8) assay, and the cell invasion and migration were tested using Transwell assay and wound-healing assay.
RESULTSCompared with the adjacent tissues, HCC tissues expressed significantly higher levels of IFITM3. In HepG2 cells, transfection with IFITM3 siRNA resulted in significant down-regulation of IFITM3 expression at both the protein and mRNA levels and obviously suppressed cell proliferation, invasion, and migration ability as compared with the cells transfected with scrambled siRNA and control cells (P<0.05).
CONCLUSIONSIFITM3, which is overexpressed in HCC, plays a vital role in the proliferation and invasion of HCC cells and may serve as a potential target for gene therapy of HCC.
Carcinoma, Hepatocellular ; genetics ; pathology ; Cell Proliferation ; Down-Regulation ; Gene Knockdown Techniques ; Hep G2 Cells ; Humans ; Liver Neoplasms ; genetics ; pathology ; Membrane Proteins ; genetics ; RNA, Messenger ; RNA, Small Interfering ; RNA-Binding Proteins ; genetics ; Transfection