Child ; Guidelines as Topic ; Humans ; Invasive Pulmonary Aspergillosis ; diagnosis ; therapy ; Lung Diseases, Fungal ; diagnosis ; microbiology ; therapy

Child ; Chronic Disease ; Cough ; diagnosis ; Humans ; Practice Guidelines as Topic

Cognition ; Language ; Medicine, Chinese Traditional ; Meridians ; Metaphor ; Translations

From July 1998 to May 2000, 31 cases of lumbar spondylolisthesis accompanied with lumbar stenosis were treated with spinal cord decompression, autograft interbody fusion and fixed by Dyna lok system. All the patients were followed up for 6 to 28 months, averaged 12 7 months. 29 of the 31 patients were recovered completely. 2 cases of cauda equina were recovered within 2 months. Lowback pain remained in 1 case of multi segements spondylolisthesis which had been fixed for 3 vertebrae. 1 patient accompanied with cervical spinal stenosis had some residual claudication. In conclusion, spondylolisthesis fixed with the Dyna lok system can achieve a satisfactory result.

Humans ; Mycoplasma pneumoniae ; Pneumonia, Mycoplasma ; epidemiology

Bronchoscopy ; Child ; Congenital Abnormalities ; pathology ; Humans ; Respiratory System ; pathology

Bronchoscopy ; Child ; Congenital Abnormalities ; pathology ; Humans ; Respiratory Tract Diseases ; complications ; pathology

Objective: To study the hypoglycemic effects of soybean isoflavones (SI) and soyasaponins (SS) in diabetes, and their inhibitory activities on alpha-glucosidase (EC 3.2.1.20) and alpha-amylase (EC 3.2.1.1). Methods: GK/Jcl type 2 diabetic rats were given diet containing 20 g/kg of soybean hypocotyl extracts (SHE) for 20 weeks, then the blood glucose and oral glucose tolerance test were observed. SI and SS were isolated by ODS column chromatography and high-performance liquid chromatography (HPLC) from SHE. The inhibitory activities of each component of SI and SS against alpha-glucosidase and alpha-amylase were tested by colorimetric method. Results: SHE decreased blood glucose significantly in type 2 diabetic rats and improved glucose tolerance in both normal and diabetic rats. In alpha-glucosidase inhibitory assay, Group B, Group E and DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins showed potent inhibitory activities with IC50 values of 10-40 靘ol/L. Isoflavone aglycones also showed potent inhibitory activities against alpha-glucosidase with IC50 values of 20-150 mol/L, while isoflavone glycosides showed a little lower potencies. Inhibition of SI and SS on alpha-amylase was low, and their inhibitory activities were about 10%~20% at the concentration of 1g/L. Conclusion: SI and SS may decrease blood glucose and improve oral glucose tolerance in diabetic rats, probably via the inhibitory effects on alpha-glucosidase and alpha-amylase.

Objective: To clone the cDNA of human sPLA2-IIA,construct the engineered Escherischia coli expressing human sPLA2-IIA and identify the expressed human sPLA2-IIA. Methods: Total RNAs were purified from human fetal spleen. The cDNA of human sPLA2-IIA was cloned by RT-PCR and inserted into plasmid pET32a(+) between NcoI and EcoRI sites for expressing the recombinant human sPLA2-IIA in Escherischia coli BL21(DE3). The recombinants were screened by SDS-PAGE. The engineered Escherischia coli expressing trxA-human sPLA2-IIA fusion protein was established. The expressed human sPLA2-IIA exists in the form of inclusion body and accounts for about 25% of the total proteins of Escherischia coli BL21(DE3). Conclusion: the engineered E. coli methods are suitable for preparing plenty of human sPLA2-IIA which has laid base for the large-scale expression,purification and basic studies of human sPLA2-IIA.

0.05), and both groups got ideal clinical effect. Conclusions MGHSV is an ideal operation for various types of duodenal ulcer, which with a preferable long-term effect. It is worth to be widely applied in clinical practice.