A bacterium(designated as LW-3), capable of degrading Chlorimuron-ethyl, was isolated from the long-term contaminated soil by Chlorimuron-ethyl. Based on physiological and biochemical analyses and sequences comparison of 16S rDNA, strain LW-3 was identified as Pseudomonas sp. LW-3 could use Chlorimuron-ethyl as sole nitrogen source for growth. The optimum pH and temperature for degradation of Chlorimuron-ethyl were pH 6.5~7.2 and 30?C~35?C, at same temperature the pH change to the Chlorimuron -ethyl degrading influence is large. When the pH and temperature were pH 6.5 and 32?C, 50 mg/L Chlori- muron-ethyl could be degraded to 70%~80% level within 7 days.

Objective To investigate the relationship of fasting serum FFA composition with central obesity, hypertension, insulin resistance, levels of serum zinc and age, gender-related differences. Methods Serum FFA were determined by high performance liquid chromatogram in 232 commuity|based subjects aged between 35 and 55 years: 109 hypertensives, 123 normotensives. Results Multivariate stepwise linear regression models and correlation analysis showed that serum FFA composition (including levels of n3PUFA, P/S ratio, C20:5/C18:3, C22:6/C20:5 and C20:4/C18:2 ratio) were linearly correlated with waist/ hip ratio, insulin sensitivity index, levels of serum zinc and related to the habit of diet (all P

ObjectiveTo investigate the economic status of psychiatric patients and their families in Shaoxing area in Zhejiang Province.MethodsA self-edited questionnaire was used to survey the annual income and expenses for medical treatment among psychiatric patients and their families.ResultsThe average annual income of per person in psychiatric family was (3823.07±3354.99) Yuan while average annual income of per psychiatric patient was (1918.20±5188.42) Yuan, which were only 62.3% and 31.2% of the rural controllable annual income, 20.9% and 14.2% of urban controllable annual income in Shaoxing area. In the meantime, 30.43% psychiatric patients did not have any incomes and 47.43% were below 500 Yuan per year. Engel coefficient was (59.65±24.36). The average expense for medical treatment was 1052.54 Yuan per year, which was 54.9% of annual income of the psychiatric patients. In some illness, this rate was as high as 90% or above.ConclusionThe incomes of psychiatric family members and psychiatric patients themselves are much lower than the local average level, while they would be spent more than a half of the income on their medical treatment. Psychiatric patients as well as their family members are the extremely low income and weak social class and need help by the government and society.

Animals ; Cell Culture Techniques ; Cells, Cultured ; Male ; Neural Stem Cells ; cytology ; Neurons ; cytology ; Olfactory Mucosa ; cytology ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo analyze the genetic relations of Shigella isolated from Shenzhen in 2001-2006 and develop primary molecular subtyping surveillance network of Shigella.

METHODSChromosomal DNAs from 55 isolated in agarose were digested with the restriction enzyme Xba I, and then were analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns were clustered using BioNumerics software.

RESULTSAll 41 distinctive PFGE patterns were identified among 55 strains. 32 strains belonged to one cluster. Differences were observed in other strains.

CONCLUSIONBoth genetic-related clones and non-related clones of Shigella existed in Shenzhen. The development of PFGE molecular subtyping surveillance network would contribute to the active surveillance, outbreak investigation and source tracking for Shigellosis.

Bacterial Typing Techniques ; China ; Electrophoresis, Gel, Pulsed-Field ; methods ; Feces ; microbiology ; Humans ; Shigella ; classification ; isolation & purification

This study was purposed to investigate the status of Id4 gene promoter methylation in malignant hematologic cell lines. MS-PCR methods were used to detect the status of Id4 gene methylation in K562, HL-60, Ramous and CA46. Meanwhile bone marrow cells in healthy individuals and Hek937, a benign renal cell line, were involved as control. The results showed that Id4 gene kept unmethylated in bone marrow cells from healthy individuals and Hek937 cells, while Id4 gene was methylated in all of K562, HL-60, Ramous and CA46 cell lines. It is concluded that different from healthy bone marrow cells and non-malignant cell line Hek937, Id4 gene was methylated in leukemia and lymphoma cell lines including K562, HL-60, Ramous and CA46. The change of Id4 gene methylation is thought to be associated with occurrence of hematologic malignancies.
Cell Line, Tumor ; DNA Methylation ; Hematologic Neoplasms ; genetics ; pathology ; Humans ; Inhibitor of Differentiation Proteins ; genetics ; K562 Cells ; Promoter Regions, Genetic

The study was purposed to investigate the significance of id4 gene promoter methylation in monitoring AML patients with complete remission (CR). Methylation specific-PCR (MS-PCR) were used to detect the status of promoter methylation of id4 gene in bone marrow samples from AML patients with CR who had accepted induction with DA or IA and 4 to 5 consolidation chemotherapy with Ara-C. The results showed that in the all 32 patients, 15 were found to show id4 promoter methylation and 7 out of the 15 were found relapsed or tendency to relapse in the following-up period. While all the 17 patients with id4 unmethylation were still in their CR status in the same period. In conclusion, id4 promoter methylation might be a predictor for relapse of AML patients with CR in certain degree.
DNA Methylation ; Humans ; Inhibitor of Differentiation Proteins ; genetics ; Leukemia, Myeloid, Acute ; genetics ; metabolism ; Neoplasm, Residual ; diagnosis ; genetics ; Promoter Regions, Genetic ; genetics

The aim of this study was to evaluate the significance of id4 gene promotor methylation detection in NHL patients. MS-PCR method was used to detect the status of id4 gene methylation in health donors and newly diagnosed NHL patients. The results indicated that the id4 gene was unmethylated in bone marrow samples from health donors. Among 18 newly diagnosed NHL patients, including one NHL patient with bone marrow cells involved, 4 patients were found in id4 gene methylation by MS-PCR. The 14 patients with id4 gene unmethylation were in their stable status and no bone marrow involvement were found by bone marrow biopsy during the 8-month follow-up. During the follow-up, the patient with both bone marrow involvement and id4 gene methylation turned to leukemia, in 2 out of the 3 patients with id4 gene methylation but without bone marrow involvement at diagnosis, the bone marrow involvement was found at last. It is concluded that the id4 gene methylation may be an indicator for MRD in NHL patients without bone marrow involvement.
Bone Marrow ; pathology ; DNA Methylation ; Humans ; Inhibitor of Differentiation Proteins ; genetics ; Lymphoma, Non-Hodgkin ; genetics ; pathology ; Promoter Regions, Genetic ; genetics

The purpose of this study was to investigate the clinical signs and therapy of primary non-Hodgkin's lymphoma of bone (PLB). The clinical symptoms, signs, X-ray features, pathological morphology, immuno-phenotype and treatment of 23 patients with PLB were analyzed retrospectively. The results indicated that the main complains of 23 cases of PLB were local pain and tenderness; the radiographic and CT findings showed invasive in 15 cases, osteolytic in 5 cases, sclerotic in 2 cases and cystiform expansion in 1 cases. Most of histological types were diffuse large B cell lymphoma; there were single bone involvement in 21 cases and multiple involvement in 2 cases; 3 cases had pathologic fracture. In conclusion, PLB usually involved single bone, roentgenography and CT showed erosive and osteolytic bone destruction. The roentgenography and CT are difficult to diagnose PLB, the final diagnosis should be confirmed according to clinical features, pathological findings and immunohistochemistry assay. The immunohistochemistry is helpful to diagnosis and identification of histological type for PLB. The therapeutic procedure for PLB mainly includes local radiotherapy combined with chemotherapy.
Adult ; Aged ; Bone Neoplasms ; diagnosis ; therapy ; Combined Modality Therapy ; Female ; Humans ; Lymphoma, B-Cell ; diagnosis ; therapy ; Lymphoma, Non-Hodgkin ; diagnosis ; therapy ; Male ; Middle Aged ; Retrospective Studies ; Young Adult

This study was aimed to analyze the clinical features, anti-fungal therapeutic efficacy and safety in hematological malignancy patients with invasive aspergillosis (IA) after hematopoietic stem cell transplantation (HSCT) or chemotherapy. The patients with hematological malignancies received chemotherapy or HSCT were analyzed retrospectively, then the clinical characteristics and diagnosis were analyzed according to the diagnostic criteria for IA. The efficacy and safety of anti- Aspergillus therapy, and the factors influencing therapeutic response were evaluated. The results showed that out of 30 cases with IA, 2 were proven, 19 were probable, 9 were possible, and 19 were diagnosed after HSCT, most in the late period after-HSCT (> 40 d). 8 cases received fluconazol only, 6 received caspofungin only, 7 received combined therapy. The efficacy and time interval from the first day of treatment to successful response (TTR) were 87.5%, 50% and 85.7% and 38, 20 and 36 days, respectively. Combined therapy is better than single drug treatment (p < 0.05) while the TTR was not significantly different between them. The factors influencing the therapeutic efficacy were as follows: age, HSCT, GVHD and CMV, previous IFI and so on (p < 0.05). All the anti- Aspergillus medicines resulted in some injury of hepatic and renal function. However, there were no significant difference between the drugs or between combination and single drug therapy (p > 0.05). It is concluded that IA is also the major and severe complication in the patients with hematological malignancies or received HSCT. Combined therapy for anti- aspergillus is better than single drug in efficacy and safety, without increasing the adverse drug reactions for hepatic and renal function. The efficacy of anti- aspergillus may be related to age, HSCT, GVHD and CMV, previous IFI and so on.
Adolescent ; Adult ; Antifungal Agents ; therapeutic use ; Aspergillosis ; complications ; diagnosis ; drug therapy ; Aspergillus ; Female ; Hematologic Neoplasms ; complications ; drug therapy ; microbiology ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Young Adult