Contents of magnolol and honokiol in Magnolia officinalis Rehd. et Wils. and its processed products,yellow-fried, burn -fried and ginger-fried M. officinalis Rehd. et Wils. as well as commercially available M.officinalis Rehd. et Wils. and its processed products were assayed by TLC Scanning. Results showed that contents of the two constituents decreased after M. officinalis Rehd. et Wils. was processed. Among them the burn-fried product showed a most pronounce decrease of 30%, while both yellow-fried and ginger-fried products decreased about 20%. The contents of magnolol and honokiol in commercial M. officinalis Rehd. et Wils. and its ginger-fried product available in other provinces and cities differed greatly with some almost contain no magnolol and honokiol.

Spermatogenesis is a complex developmental process in which a diploid progenitor germ cell transforms into highly specialized spermatozoa. During spermatogenesis, membrane remodeling takes place, and cell membrane permeability and liquidity undergo phase-specific changes, which are all associated with the alteration of membrane lipids. Lipids are important components of the germ cell membrane, whose volume and ratio fluctuate in different phases of spermatogenesis. Abnormal lipid metabolism can cause spermatogenic dysfunction and consequently male infertility. Germ cell membrane lipids are mainly composed of cholesterol, phospholipids and glycolipids, which play critical roles in cell adhesion and signal transduction during spermatogenesis. An insight into the correlation of membrane lipids with spermatogenesis helps us to better understand the mechanisms of spermatogenesis and provide new approaches to the diagnosis and treatment of male infertility.
Cell Adhesion ; Cell Membrane ; chemistry ; Cholesterol ; chemistry ; Glycolipids ; chemistry ; Humans ; Infertility, Male ; Male ; Membrane Lipids ; chemistry ; Phospholipids ; chemistry ; Signal Transduction ; Spermatogenesis ; Spermatozoa ; cytology

Sulfogalactosylglycerolipid (SGG) is the main glycolipid in male mammalian germ cells, which is selectively and highly expressed in mammalian testes and helps form the lipid bilayer of cell membrane. In the process of spermatogenesis, SGG is involved in the meiosis of spermiocytes. Either deficiency or accumulation of SGG will lead to male infertility. SGG homeostasis in the testis is the premise of normal spermatogenesis. In the process of sperm-zona binding, SGG becomes a component of lipid raft and provides a platform for signal transduction. The SGG binding protein plays a role in sperm-egg recognition and membrane fusion. SGG has a great research value and application prospect in male reproduction.
Animals ; Cell Membrane ; Galactolipids ; physiology ; Humans ; Infertility, Male ; etiology ; Lipid Bilayers ; metabolism ; Male ; Signal Transduction ; Sperm-Ovum Interactions ; physiology ; Spermatogenesis ; physiology ; Spermatozoa ; metabolism ; Testis ; physiology

Being expert,sensitive and quick-responding,biosensor is widely applied in medicine domain.Theis paper briefly introduces the structure and characteristics of biosensors,analyzes the framework and principle of some types of biosensors and their applications in the biomedicine engineering,and discusses the recent research evolvement,development trend and application prospects.

Objective Aimed at the problems that the costs of existing ECG data compression methods is high,and they are difficult to apply in engineering practice,a sort BP neural network is set up based on ECG data compression method.Methods Based on BP network theory,two three-layered feedforward neural networks were set up.Then every one heartbeat was divided into three waves,that is,P,QRS and T ones,and the three waves were compressed by two three-layered feedforward neural network individually.In order to improve the replay capability and interference rejection capability of the neural network compress algorithm,incompletely connected structure is employed.Results The method could realize high compress ratio,and improve the replay capability and interference rejection capability of the heartbeat waves.Conclusion Upon with the heartbeat signals,the method can filter and compress waves effectively,and can be used in engineering practice as well.

Polymalic acid, known as a bioactive material, is completely biodegradable, and has far reaching application potential in medical field. Combined with our own findings, we summarized advances in polymalic acid metabolism, microbial fermentation synthesis, and application research in the medical field. Finally, prospect for further research was addressed.
Fermentation ; Industrial Microbiology ; Malates ; chemistry ; Polymers ; chemistry

Clinical obstetrics practice is a very important process. According to our exploration on it on 7-year-systemmedical students in our hospital for nine years, we think that if you want high quality 7-year-system medical students,five concepts should be emphasized besides traditional basic knowledge, basic theory and basic skill, which are familyplanning and eugenics, loving infants, whole concept, new medical model and renewing knowledge.

ObjectiveTo evaluate the mid-term clinical effect of nano-hydroxyapatite and polyamide 66 (n-HA/PA66) intervertebral fusion cage in treatment of thoracolumbar burst fractures.Methods A total of 87 patients with thoracolumbar burst fractures were managed by thoracolumbar body resection combined with n-HA/PA66 intervertebral fusion cage from December 2007 to September 2008.The clinical effect, safety and radiographic outcomes were evaluated.Results No nerve damage was deteriorated in all the patients.The neural function was improved for 1-2 grade except for four patients at Frankel grade A.The patients were followed up for mean 21.3 months (17-24 months).The kyphosis was (14.4 ± 12.6)° preoperatively, (3.7 ± 8.7) ° immediately after surgery and (4.0 ± 8.3)° at final follow-up.The distance between the upper and lower vertebral bodies was (96.9 ± 17.2) mm preoperatively, (109.5 ± 17.1) mm immediately after surgery and (108.3 ± 16.4) mm at final follow-up.No cage replacement, internal fixation breakage or neurologic impairment were observed during follow-up period.There were 58 patients with grade E fusion, 22 with grade D fusion and 7 with grade C fusion.ConclusionsAnterior decompression combined with n-HA/PA66 intervertebral fusion cage is an effective method for treatment of thoracolumbar burst fracture.The kyphosis is rectified and the intervertebral distance is corrected, with a high rate of fusion.

AIM:To investigate the influence of F10 on the activities of transcription factor NF-?B and AP1 in A549 cells.METHODS:The luciferase report plasmids of NF-?B-Luc,AP1-Luc and F10 gene were introduced into A549 cells and the luciferase activity was detected.The DNA binding activities of AP1 and NF-?B in the cells were measured by the electrophoretic mobility shift assay(EMSA).RESULTS:The luciferase activity in F10+ transfection group decreased 30% and increased 2-fold respectively 48 h after transfected with the luciferase report plasmid of NF-?B-Luc and AP1-Luc in A549 cells.The DNA binding activity decreased 49% and increased 23%,respectively.CONCLUSION:F10 gene up-regulates the transcription activity of AP1 and down-regulates the NF-?B in A549 cells.

Child ; Cross Infection ; Humans ; Pneumonia