Objective To investigate the prognostic factors in patients with sepsis and to provide the theoretical basis for clinical diagnosis and treatment. Methods A total of 141 septic patients were analyzed retrospectively. Patients were divided into two groups:those who were alive at time of ICU discharge ( survivors;n=83 ) and those who died or to give up before terminal stage ( nonsurvivors;n=58 ) . Then the clinical data were statistically ana-lyzed. Results In the death group: age, APACHE II score, blood urea nitrogen, serum creatinine, C-reactive protein were significantly higher than the survival group (P<0. 01) while albumin, pre albumin were lower than that in the survival group (P<0.01). The Logistic regression analysis illustrated: age ( > 60 years), APACHEIIscore ( >20 score) and blood urea nitrogen ( > 9 mmol/L) , serum creatinine ( > 176 μmol/L) , C-reactive protein level were independent predictors of death in patients with sepsis ( P<0 . 05 ) . Then drew the receiver oper-ating characteristic curve ( ROC curve) and analyzed the respective risk factor for predicting prognosis value of sep-sis with the help of ROC curve. Conclusion Age, high APACHE II score in early sepsis, increased urea nitro-gen, elevated serum creatinine and increased C-reactive protein levels were regarded as prognostic value indicators for adverse clinical outcomes in patients with sepsis.

Acromegaly causes many complications such as bone and joint diseases, sleep apnea syndrome, glucose and lipid metabolism disorders, cardiovascular diseases, and tumors. Delays in clinical diagnosis and treatment have significantly increased the incidence of complications. According to " Chinese Expert Consensus on the Diagnosis and Treatment of Acromegaly(2020)" , we interpret the assessment, management, and follow-up of acromegaly comorbidities in order to guide and standardize the management of acromegaly comorbidities and improve the prognosis of patients.

Adolescent ; Child ; Child, Preschool ; Critical Care ; Fatty Acid-Binding Proteins ; blood ; metabolism ; urine ; Female ; Gastrointestinal Diseases ; blood ; urine ; Humans ; Male ; Metabolic Diseases ; blood ; urine

Snake antivenomimmunoglobulins are considered to be the most efficient drugs in snake envenomings. Most snake antivenomimmunoglobulins all over the world are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum till now. In this review, we retrospect the history of snake antivenomimmunoglobulins, analysis the present situation and pay the close attention on the key technological links in the process of research and manufacturing, such as properties of IgG and its fragments, selection and preparation of immunogen, optimization of immunization schedule and protein isolation and purification, which can be available for the reference in the research and development of snake antivenom.
Animals ; Antivenins ; pharmacology ; Humans ; Immunoglobulin G ; pharmacology ; Snake Bites ; drug therapy ; Snakes

Objective To explore the potential significance of long noncoding RNA (lncRNA) expressed in endoplasmic reticulum stress response also known as unfolded protein response ( UPR) .Methods Mouse embryonic fibroblasts ( MEFs) were isolated from 13 day mouse embryo , cultured and treated with tunicamycin for 16 hours to induce UPR .The untreated MEFs were used as negative control .The lncRNA expression profile was examined by a customized lncRNA array. The results of array assay were validated by real-time PCR and further analyzed by bioinformatics. Results There were 411 lncRNAs whose expression was signficantly up-regulated in MEFs treated with tunicamycin as compared with the controls ,while 790 lncRNAs were significantly down-regulated. A number of significantly alter lncRNAs were validated by real-time PCR. A further bioinformatics analysis of lncRNA profile suggested that many cold be in-volved in multiple cellular pathways. Conclusion Our lncRNA expression profile and bioinformatics studies srongly suggest that many lncRNAs can be regulated in the cells under UPR and could also in turn regulate UPR and other cellular processes.

The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58～151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.

Adenoma, Pleomorphic ; surgery ; Humans ; Parotid Neoplasms ; surgery

Objective To investigate the influence of HCV core protein on cell apoptosis, cell cycles and cell telomerase activities of HepG2 cells. Methods A eukaryotic expression plasmid containing HCV C gene was constructed by DNA recombinant technique and the recombinant plasmid was transfected into HepG2. Thereafter, HepG2 cells transfected with recombinant eukaryotic expression plasmid were obtained. The HCV C mRNA and protein in HepG2 cells transfected with recombinant plasmid were verified by RT PCR and indirect immunofluorescence assay. The HepG2 cells were studied as follows: (1) The cell proliferation ratio of three groups cells(HepG2 cells transfected with the recombinant plasmid, HepG2 cells transfected with blank plasmid and HepG2 cells without transfection) was evaluated by MTT assay; the cell cycles were also examined by FACS. (2) The apoptotic ratio of three groups cells were examined by FACS. (3) The cell telomerase activities of all three group cells were examined by TRAP ELISA assay. Results (1) The cell proliferation ratio in the group of HepG2 cells transfected with recombinant plasmid was higher than that of the group of HepG2 cells transfected with blank plasmid or the group of HepG2 cells without transfection; The proportion of phase S in the group of HepG2 transfected with the recombinant plasmid was significantly higher than that of the group of HepG2 without transfection. (2) The apoptotic ratio in the group of HepG2 cells transfected with recombinant plasmid was significantly lower than that of the group of HepG2 cells transfected with blank plasmid or the group of HepG2 cells without transfection. (3) There were no significant differences among the three group cell telomerase activities. Conclusions (1) HCV C protein had the potential role in inhibiting cell apoptosis. (2)HCV C protein could induce HepG2 cells from phase G 0/1 to phase S, and might promote cell proliferation, inhibit cell apoptosis. (3) HCV C protein had no influence on cell telomerase activities of HepG2 cells, thus HCV C protein regulated cell cycle, promoted cell proliferation and inhibited cell apoptosis not by enhancing cell telomerase activities.

Animals ; Disease Models, Animal ; Sleep Deprivation ; physiopathology ; psychology