Objective To investigate the expression and clinical significance of the long non-coding RNA MALAT-1 in epithelial ovarian cancer (EOC).Methods The expressions of MALAT-1 mRNA in 29 EOC tissues and 20 normal ovarian tissues were detected by real-time fluorescence quantitative PCR.The relationships between the expression of MALAT-1 mRNA in EOC and its clinical pathological characteristics were analyzed.Results The expression of MALAT-1 mRNA in EOC tissues was lower than that in normal ovarian tissues (0.089±0.090 vs 1.450±1.404,t =4.331,P ＜ 0.001).The expression of MALAT-1 mRNA in advanced (Ⅲ-Ⅳ) stage tissues was significantly lower than that in early (Ⅰ-Ⅱ) stage tissues (0.044±0.043 vs 0.174±0.095,t =-5.125,P ＜ 0.05).The expression of MALAT-1 mRNA was significantly lower in poorly differentiated and poorly-moderately differentiated ovarian cancer than that in well differentiated ones (0.070 ±0.091 vs 0.161±0.021,t =2.409,P =0.023).The expression of MALAT-1 mRNA was significantly lower in tissues with the lymphonode or omental metastasis than that in tissues without invasion (0.045±0.047 vs 0.142±0.102,t =3.378,P =0.002).Conclusions The expression of long non-coding RNA MALAT-1 is lower in EOC,which is related to tumor grade,clinical stage,lymphonode and omental metastasis.It is suggested that MALAT-1 may play an inhibition role in the progress of ovarian cancer.

Sixty two patients with small bowel bleeding underwent double balloon endoscopy (DBE).Clinical features were compared between patients with positive DBE findings (50 cases) and those with negative findings (12 cases).Results revealed that the causes of melena were discovered in 75.0％(21/28) patients via oral approach,while the lesions were found in 81.8％ (18/22) patients with hematochezia via anal approach ( P ＜ 0.01 ). Duration of bleeding was longer and number of bleeding episodes was higher in patients with positive DBE findings than those with negative findings (P ＜ 0.05 or P ＜ 0.01 ).The time interval between the latest bleeding and DBE was significantly shorter in patients with positive DBE findings (P ＜ 0.05).Positive diagnostic rate was significantly higher in patients with three or more bleeding episodes than those less than three times (92.5％ vs.11.1％,P ＜ 0.01 ).DBE performed within seven days or less increased the positive diagnostic rate significantly than that after seven days (97.1％ vs.59.3％,P＜0.01).