Objective To investigate clinical and imaging features of malignant transformation in monostotic fibrous dysplasia (FD).Methods Radiography (n=12),computed tomography (CT)(n=7)and magnetic resonance imaging (MRI)(n=5)data of 12 monostotic FD cases proved by surgery and pathology were retrospectively reviewed.Results Among 12 cases,6 cases underwent surgery.Their images showed osteolytic lesions in the operative area with direct signs of aggressiveness (poorly defined margin,cor-tical destruction and soft tissue mass).For the 6 cases without surgery,radiography and CT showed poorly margin,osteolytic le-sions within or near the area of ground-glass opacity.MRI showed lesions with heterogeneous signal intensity and pronounced con-trast-enhancement.Histopathologically,9 cases were osteosarcomas,2 cases were fibrosarcomas,and 1 case was malignant fibrous histiocytoma (MFH).Conclusion The imaging information of malignant transformation of FD can provide evidence of possible ma-lignancy.

Objective To investigate the change of lung function in children with atypical asthma and different lung function index to evaluate the difference of bronchial dilation test positive rate.Methods Seventy-one cases of atypical asthma children routine monitoring lung function,underwent bronchial dilation test,the airway index changes were observed.Results Seventy-one cases of atypical asthma children with big airways function index of forced expiratory volume in first second FEV1,percentage of FEV1 to forced vital capacity (FVC) were basic normal,and the reaction of small airways function index of forced expiratory flow at 25％ of FVC,forced expiratory flow at 50％ of FVC,forced expiratory flow at 75％ of FVC,forced expiratory flow at 25％-75％ of FVC were decreased in different degrees,and mainly for medium and severe damage.FEV1 as evaluation index,the positive rate of bronchial dilation test diastolic was 47.9％ (34/71),and forced expiratory flow rate at 25％-75％ in FVC as evaluation index,the positive rate of bronchial dilation test was 69.0％ (49/71),there was significant difference (x2 =8.086,P =0.004).Conclusions Lung function changes in atypical asthma children are mainly on small airways,and mainly for medium and severe damage,the bronchial dilation test positive rate in such children using the evaluation,forced expiratory flow rate at 25％-75％ in FVC can assist FEV1,decrease the rate of misdiagnosis and missed diagnosis.

The action of BTHP on a-adrenoceptor was studied in anococcy-geus muscles, vas deferens and cell membrane of cerebral cortex of rats and aortic strips, saphenous vein of rabbit. BTHP produced parallel shifts to the right of dose-response curves for phenylephrine in rat anococcygeus muscle and rabbit aortic strips without change in the maximal responses. Their pA2 values were 5.8 and 5.86 respectively. BTHP was also a competitive antagonist for presynaptic and postsynaptic ?2-adrenoceptors on prostatic portion of rat vas deferens( pA2 against clonidine=5.2 ) and on rabbit saphenous vein ( pA2 against B-HT920=5.3 ) . BTHP decreased in dose-dependent manner 〔8H〕 prazosin and 〔3H〕 clonidine specific binding to ?1- and ?2- adrenoceptors on cell membrane of cerebral cortex in rats with IC50 of4 and 9.5 ?mol/L and with Ki ( apparent dissociation constant ) of 3.5 and 8.1?mol/L respectively, suggeating that BTHP has affinity for both ?1 and ?2- adrenoceptors of rat cerebrance.

Objective To analyze the clinical characteristics of patients with L isteria septicemia for better clinical diagnosis and management of the disease .Methods A retrospective study was carried out in 13 patients with confirmed diagnosis of Listeria septicemia from July 2009 to November 2013 in West China Hospital of Sichuan University .The clinical features ,laboratory tests ,treatments and clinical outcomes were reviewed and analyzed . Results The vast majority of the 13 patients were immunocompromised or with critical organ dysfunction . The in vitro antimicrobial susceptibility testing indicated that penicillin ,ampicillin and levofloxacin were the most active agents against Listeria ,followed by imipenem ,erythromycin , ciprofloxacin and tetracycline .Only 33 .3% of the 13 Listeria isolates were sensitive to oxacillin .Eight patients were cured ,2 improved ,2 died after therapy .The remaining one patient gave up therapy .Conclusions The incidence of Listeria septicemia was associated with advanced age and presence of underlying diseases .Early etiology diagnosis and appropriate antibacterial therapy can improve the outcome of such patients .Actively treating underlying diseases helps reduce the mortality rate .

Interleukin-22 (IL-22) is a member of the IL-10 family, which exhibits a similar structure and immune function with IL-10. IL-22 is mainly secreted by Th22, Th1, and Th17 cells, innate lymphoid cells (ILCs), and γδT and NKT cells. IL-22 is mainly in-volved in autoimmune diseases, malignant tumors, and infective diseases. As such, studies have focused on the function of IL-22 cyto-kine in the pathogenesis of various malignant tumors. Studies revealing the expression and pathogenesis of IL-22 in malignant tumors possibly provide a new method to treat and prevent malignant tumors. In our study, the discovery, structural characteristics, and func-tions of IL-22 in malignant tumors are reviewed.

Objective To investigate the image characteristics and clinical application of 18F-FDG PET/CT in autoimmune pancreatitis (ALP).Methods The PET/CT images from six male patients (age ranging from 51 to 78(average 69) years) with AIP from 2005 to 2012 were studied retrospectively.Of the six patients,two had follow-up PET/CT images after steroid therapy.The morphologic abnormality was visually analyzed and SUV was calculated.Scores were obtained according to the SUV of pancreas compared with that of the liver (3 =SUV higher than liver,2=SUV similar to liver,1 =SUV lower than liver).The difference between the regular and delayed SUV was compared by paired t test using SPSS 17.0.Results All of the 6 patients showed diffuse FDG uptake in the entire pancreas with SUVmax of 3.2-6.0(5.2± 1.1).Five patients had score 3 and one had score 2.Five patients had delayed scan,of which 4 had increased uptake (SUVmax5.3-7.2),but the SUVmax was not significantly different compared to that before delay scan (4.8-6.0,t =-2.424,P＞0.05).Five patients showed extrapancreatic uptake,especially in the salivary glands.After the steroid therapy,the enlarged pancreas reduced and the intense uptake of the pancreas disappeared.The extrapancreatic uptake showed coinstantaneous remission.Conclusions Increasing FDG uptake at entire pancreas was observed in patients with AIP.18F-FDG PET/CT may be useful for detecting AIP and the associated extrapancreatic uptake,and monitoring the change after therapy,yet it needs further evaluation.

To establish a pig kidney cell line LLC-PK1/BCRP in which human breast cancer resistance protein was highly expressed, the expression vector pcDNA3.1(+)-BCRP which contained BCRP gene was constructed and transfected into LLC-PKI cells via liposomes. After selecting with G418, population doubling time, flow cytometry and Western blotting analysis were used to evaluate the cell line. MTT assays were employed to determine the drug resistance index of mitoxantrone and doxorubicin. Invert fluorescent microscope was used to observe the efflux of fluorescence dye Hoechst 33342 by BCRP, furthermore, the BCRP's inhibitor GF120918 was applied to reverse the efflux of Hoechst 33342. The experiment results showed that the expression of BCRP protein increased in LLC-PK1/BCRP cell. The population doubling time of LLC-PK1/BCRP cell was a little longer than that of the parental cell LLC-PK1. The resistance indexes to mitoxantrone and doxorubicin were 51.95 and 6.09 times, respectively, higher than LLC-PK1 cell. The efflux of Hoechst 33342 was significantly enhanced and could be reversed by GF120918. So a LLC-PK1/BCRP cell line was established, which highly expressed BCRP protein successfully. This cell line could be a valuable model to further investigate the biological profile of BCRP and select the substrate and inhibitor of BCRP.

Objective To analyze the imaging characteristics and the diagnostic value of conventional MRI and indirect MR arthrography in shoulder joint injury.Methods MR imaging in90 patients with shoulder joint disease were retrospectively analyzed by two musculoskeletal radiologist based on the results of arthroseopic examination,including 57 cases of rotator cuff disease and 33 cases of recurrent shoulder instability.The result of the indirect MR arthrography and conventional MR was statistically compared by Fisher exact probability test.Results (1)In the group of rotator cuff disease,37 patients underwent indirect MRA,demonstrating 10 cases of partial-thickness rotator cuff tear and 1 case of missed diagnosis;demonstrating 17 cases of full-thickness rotator cuff tear and 2 cases of misdiagnosis.For partialthickness rotator cuff tear,the sensitivity,specificity and accuracy of indirect MR arthrography were 90.9%(10/11),92.3%(24/26),and 91.9%(34/37),respectively;for full-thickness rotator cuff tear,the sensitivity,specificity and accuracy of indirect MR arthrography were 89.5%(17/19),94.4%(17/18),and 91.9%(34/37),respectively.Indirect MR arthrography in the diagnosis of partial and full thickness rotator cuff was significantly better than conventional MR(P＜0.05).(2)In the group of recurrent shoulder instability,labium tear was found in 26 cases,Hill-sachs lesion in 27,reverse Hill-sachs in 2,bony Bankart lesion in 7,and capsular tear in 18 cases,respectively.Twenty patients underwent indirect MRA,demonstrating 14 cases of labrum tear,1 case of missed diagnosis,and 5 cases of normal labrum.For labrum tear,the sensitivity,specificity and accuracy of indirect MR arthrography were 93.3%(14/15),100.0%(5/5),and 95.0%(19/20),respectively.Indirect MR arthrography in the diagnosis of labrum tear was significantly better than conventional MR(P＜0.05).Conclusion MRI,especially indirect MR arthrography,is the most helpful method for the diagnoses of shoulder joint injury.

Objective To evaluate the diagnostic value of indirect MR arthrography(MRA) and conventional MR for shoulder instability.Methods 24 cases of shoulder instability were retrospectively analyzed.13 patients underwent conventional MR,11 patients underwent indirect MRA.Based on the results of arthroscopic examination,the efficacy of indirect MRA images and conventional MR images in diagnosis of the labrum,the capsule, and the bone structure injury.Results Indirect MRA in diagnosis of labrum tear was signifiantly better than conventional MR(P0.05).Conclusion Indirect MRA is more helpful to diagnose the labrum tear than conventional MR.Further study should be taken for capsule and ligament injury.

Experiment Ⅰ. Twenty-eight rats were randomly assigned into Near-Infrared Information Radiation (NIIR) group and control group. Two weeks later each rat was innoculated intraperitoneally with Salmonella typhosa H antigen (HAg) and cyclophosphamide (CY). Peripheral lymphocyte counts in the NIIR group were significantly higher than those in the control group on the fifth day after administration of CY. Experiment Ⅱ, Fifty-four rats were randomly divided into NIIR group given CY and H Ag intraperitoneally, CY and H Ag group and H Ag group for treatment. By the end of the fourth week, the survival rate and serum IgG level in the NIIR group were significantly higher than those in the CY and HAg group. By the end of 2nd week, the titer of the anti-H antibody of the HAg group and NIIR group was significantly higher than that of the CY and HAg group. Experiment Ⅲ. Thirty rats were randomly allocated to NIIR group and control group. Spleen cells were taken and cultured with Con A for 24h to induce IL-2 and the activity of IL-2 in the NIIR group was significantly higher than that in the control group. The NK activity in NIIR group was higher but not significant and ADCC in the NIIR group was significantly higher than that in the control group. The results suggest that NIIR is capable of enhancing immunoreaction in immunosuppressive bodies by promoting the function recovery of T helper cells, therefore NIIR is effective to regulate the immunological function on chronic active hepatitis.