Objective To seek a operation method of quality control of biochemical tests in peacekeeping mission via perspective of evidence-based laboratory medicine .Methods Literatures related to quality control of biochemical tests in international peace-keeping were referred ,compared and discriminated .Combined with 5-year peacekeeping laboratory experience ,a set of feasible ap-proaches were worked out and a practice evaluation was performed in a task period .Results Under the supervision of the quality control system ,providing accurate and reliable test results to the clinical ,developing and implementing quality control of biochemical tests were economical ,practical and reliable ,and could guarantee the effectiveness of reports of biochemical test in peacekeeping medical institutions .Conclusion Evidence-based laboratory medicine is important for quality control of biochemical tests in peace-keeping medical institutions .

Objective To survey the status of nosocomial infection after total knee arthroplasty,analyze the risk factors of nosocomial infection and possible prevention measures.Methods Datas were collected retrospectively on 80pmients (80 knee joints) who were treated by total knee arthrophsly,the patients were divided into two groups,group A with nosocomial infection and group B without nosocomial infection.Statistic patient's age,basic diseases situation,preoperative hemoglobin content,serum albumin,operation time,blood transfusions,indwelling urethral catheter time,antibiotic treatment time of the two groups.And study the location,pathogenic bacteria and outcomes of the nosocomial infection patients.Results 10 patients occured nosocomial infection,the infected site in turn is urinary tract in 5 cases,respiratory tract 4 cases,skin infections in 1 case,the incidence of nosocomial infection is 12.5％.In noscomial infection group,patient's age,blood transfusions,operation time and postoperative indwelling urinary canal time significantly higher than no nosocomial infection group,anemia,hypoalbuminemia have relevance of nosocomial infection,there is no difference between the two groups in basic diseases situation.Conclusions The nosocomial infection after total knee arthroplasty caused by multiple factors,patient's age,hypoalbuminemia,anemia,operation time and indwelling urethral catheter time is closely related with nosocomial infection

ObjectiveThe present study aimed to determine whether or not dual paroxysm proliferator-activated receptor (PPAR) agonist,WY14643,improved the dysfunctioned vascular endothelium in hypertension by reducing endothelium-derived contracting factors ( EDCFs ),and to explore the molecular mechanism it was involved in.MethodsIsometric tension in isolated thoracic aortic rings of spontaneously hypertensive rats was recorded.Endothelium-dependent contractions evoked by acetylcholine in the presence of L NAME were reduced by fenofibrate.Cyclooxygenase 1 ( COX1 ) activities were determined by analyzing the peroxidase activity of cyclooxygenase colorimetrically by using ELISA kit.ResultsCompared to the control group,WY14643 significantly decreased the vasoconstriction in aorta of the SHR rats(P=0.014).PPARα antagonist MK866 enhanced the vascular contractility of SHR rats that were incubated with 10.0μmol/L WY14643( P=0.021 ).PPARΥ antagonist GW9662 did not significantly affect the vascular contractility of SHR rats that were incubated with 10.0 μmol/L WY14643( P=0.061 ).The levels of serum PGFlα(P=0.012),2α( P =0.019) and TXB2(P=0.023) in SHR rats incubated with 10.0 μmol/L WY14643 were significantly lower than the control group,respectively.Under the condition of the existence of vascular endothelium,the expression of COX-1 in SHR rats incubated with WY14643 was significantly lower than that in SHR rats incubated without WY14643 (P=0.017).ConclusionsThose data showed that WY14643 reduced the release of EDCFs,it suggests that WY14643 protects against vascular diseases through the PPAR activators in spontaneous hypertension.

This paper reviews the core technology of proteomics, namely separation, identification and bioinformatics prediction of proteins, and its development and application in schistosome research.

[Objective]To analyze the reasons and counter treatment of complication after prosthetic disc nucleus implantation.[Method]From Feb 2002 to Aug 2004,80 patients of lumbar disc herniation were implanted with one single PDN device at our centre.65 patients of them came to the clinical,functional,and radiographic follow-up examinations,magnetic resonance images and follow-up records of all patients were reviewed carefully.They were divided into three groups.Group A:30 cases had at least 24 months follow-up data.Group B:23 cases had at least 36 months follow-up data.Group C:12 cases had at least 48 months follow-up data.[Result]After implantation,device migrations were noticed in 3 patients.24 cases experienced low back pain.In 24~48 month follow-up,the MR images of 39 patients had shown the abnormal signal pattern of the endplate where PDN-solo was implanted,even 32 of them had been found subsidence.In Group A,the height of intervertebral disc at the 24 month follow-up was decreased 4%.In Group B,at 36 month follow-up,it was decreased 12%.In Group C,at 48 month follow-up,it was decreased 18%.[Conclusion]After follow-up,the clinical effect of PDN is preferred.However,the complications of device dislocation,device subsidence remain problems that must be highlighted.This study suggested that the complications of PDN are serious.Therefore,the proper selecting patients for PDN replacement are crucial for good results.The indications for PDN must be restricted.

Objective To explore the ability of revascularization of big section deproteinized bone (DPB) allograft combining calcium phosphate cement (CPC) with recombinant human vascular endothelial growth factor (rhVEGF) and bone morphogenetic protein-2 (rhBMP-2). Methods Totally 36 adult hybrid dogs were inflicted to demi-articular defect models by exsecting 30 mm right femur inferior extremity near articular surface,and then randomly divided into 3 groups, group A, receiving material CPC/rhVEGF/rhBMP-2/DPB transplantation into the demi-articular defect, group B, material rhVEGF/rhBMP-2/DPB transplantation, and group C with simple material DPB transplantation. At 4, 8, 12 and 16 weeks after operation, vasculogenesis and new bone formation were assessed by X-ray examination, histological examination, transmission electron microscopy, vas capillare analysis by vaso-filling with ink and radionuclide bone imaging examination (ECT). Results The bone formation and vascularization was significantly superior in group A to that in group B and C. The results of vaso-filling examination with ink and arterial perfusion of ECT examination were both significantly superior to that in group B and C (P

Objective To study the function of hepatocyte growth factor/scatter factor(HGF/SF) in the (proliferation) of colorectal cancer cells.Methods The expression of c-met,the receptor of HGF,was(detected) in Caco-2 and Colo320 cell lines by Western blot.The activation of p42/p44MAPK and p38MAPK induced by HGF in these two cell lines was observed.Observation of the effect of the inhibitor of p42/p44MAPK(PD98059),p38MAPK(SB203580) on the inhibition of HGF-induced proliferation of Caco-2 and Colo320 cells were made by Using -TdR,MTT assay.Results(1)Both cell lines(expressed) the c-met.(2)HGF activated p42/p44MAPK and p38MAPK,and 20ng/ml HGF treated cells showed maximum activity in both to be within 10min.(p42/p44MAPK,2.28?0.01;p38MAPK,2.25?0.01).(3)HGF was found to significantly increase thymidine incorporation(P

Objective To understand the prevalence rate of mild cognitive impairment(MCI) of the aged people in the communities of Taiyuan,and to provide evidence for early diagnosis of Alzheimer's disease(AD). Methods Using a simple mental state examination(MMSE),2895 aged people of over 60 years from three communities of Wanbailin in Taiyuan were selected by cluster random sampling method.The MCI elderly patients screened were investigated further by using community health status questionnaire.Results The average MCI prevalent rate of the aged people was 7.6%;Age,marital status and education background significantly affected prevalent rate(P

OBJECTIVE:To establish a RP-HPLC method for the determination of glycyrrhizic acid in sulfonamideglycyrrhiza mixture METHODS:Lichrosorb RPC18 analytical column was used The mobile phase was composed of methanol-(CH)4NH4OH(65∶35) The pH of the mobile phase was adjusted to 6 5 with 10% phosphoric acid Detection wavelength was 254nm RESULTS:Linearity was good in the range of 0 5～50?g/ml(r=0 9 996);the average recovery was 99 75%,RSD=1 87% CONCLUSION:The method is simple and convenient

Objective To explore the change of glycogen synthase kinase-3(GSK-3) activity in lymphocytes in patients with mild cognition impairment(MCI).Methods The phosphorylation of GSK-3? at Ser 9 site and total level of GSK-3? in peripheral blood were measured by Western-blot in normal control group,one-year MCI group,two-year MCI group and three-year MCI group.Results The phosphorylation of GSK-3? at Ser 9 site were lower in 1-year MCI group,2-year MCI group and 3-year MCI group,compared with normal control group(all P0.05).Conclusion The activity of GSK-3 in lymphocytes in MCI patients is raised,which implies a subsidiary value of MCI diagnosis.