Objective To discuss the clinical preventation and treatment of the complications induced by endotracheal intubation in SICU.Methods Retrospective analysis was used on 55 cases of intubated patients in our hospital nearly 2 years.Results The incidence of endotracheal intubation was 36.4％,pneumonia 23.6％,accidental extubation 5.5％,endotracheal ulcer 1.8％,tracheoesophageal fistula 1.8％,obstruction of tracheal catheter 1.8％,arytenoid dislocation 1.8％.Conclusion The causeof complications of endotracheal intubation in SICU were overtime intubation,tranma,excessive-pressure of the air cuff and local inflammation.Airway humidification,suitable sedation,accurate and moderate operation,extubation or tracheotomy,suitable cuff pressure and fiberoptic bronchoscopy examination could avoid such complications.

Objective To seek a operation method of quality control of biochemical tests in peacekeeping mission via perspective of evidence-based laboratory medicine .Methods Literatures related to quality control of biochemical tests in international peace-keeping were referred ,compared and discriminated .Combined with 5-year peacekeeping laboratory experience ,a set of feasible ap-proaches were worked out and a practice evaluation was performed in a task period .Results Under the supervision of the quality control system ,providing accurate and reliable test results to the clinical ,developing and implementing quality control of biochemical tests were economical ,practical and reliable ,and could guarantee the effectiveness of reports of biochemical test in peacekeeping medical institutions .Conclusion Evidence-based laboratory medicine is important for quality control of biochemical tests in peace-keeping medical institutions .

Aneurysm ; diagnosis ; Carotid Artery, Internal ; pathology ; Diagnostic Errors ; Female ; Humans ; Hypertrophy ; diagnosis ; Middle Aged ; Palatine Tonsil ; pathology

Objective: To study the influence of screening dementia with mini-mental state examination (MMSE) combining with delay memory test . Methods: MMSE and delay memory test are used in screening 301 residents. The diagnosis of dementia is according to the DSM-Ⅲ-R criterion. The validity and reliability were studied when the MMSE and delay memory test were used to screen dementia alone or combined. Results: When MMSE was used to screen dementia alone, the specificity, sensitivity, false positive proportion and false negative proportion were 85.40%, 52.43%, 47.56% and 14.60% respectively. When delay memory test was used to screen dementia alone, the specificity, sensitivity, false positive proportion and false negative proportion were 74.45%, 92.07%, 7.93% and 25.55% respectively. If parallel connection of MMSE and delay memory test were used to screen dementia, the specificity, sensitivity, false positive proportion and false negative proportion were 95.62%, 49.39%, 50.61% and 4.38% respectively. If MMSE was in series with delay memory test, the specificity, sensitivity, false positive proportion and false negative proportion of screening dementia were 64.23%, 95.73%, 4.27% and 35.77% respectively. Conclusion: The parallel connection of MMSE and delay memory test can elevate sensitivity of screening dementia and decrease false negative proportion, so it is important in epidemiologic investigation. MMSE in series with delay memory test will raise specificity of screening dementia and decrease false positive proportion, so it is useful for diagnosing early dementia.

Ribosome-inactivating proteins(RIPs), a natural anti-caner protein from some advanced plants, includes single-chain robosome-inactivating proteins (type Ⅰ) and double-chain ribosome-inactivating proteins (type Ⅱ). Gelonin, relative molecular weight around 30?103, belongs to single-chain ribosome-inactivating proteins. This review is about the structure and biological activity of cell toxin Gelonin and application prospect in anti-cancer treatment.

Objective:To prepare and purify recombinant human zona pellucida 3 protein,and to study its immunologic activity.Methods:The E.coli BL21 containing recombinant plasmid pGEX4T-1/ huZP3 was induced to express GST-fusion protein by IPTG.After a series of purification procedure,the purified protein was analyzed by SDS-PAGE.After the mice immunized with rhuZP3,the antibody responses against rhuZP3 were detected by ELISA.Results:The soluble fusion protein was expressed,and purity of rhuZP3 was 95%.Moreover, purity of rhuZP3 could be recognized by anti-human ZP3 in ELISA.Conclusion:The rhuZP3 is obtained through the preparation of prokaryotic expression system and anti-rhuZP3 antibody has immunological activity.

Objective To investigate roles of Homocysteine induced gene HCY2 in vascular smooth muscle cells (VSMC) Methods HCY2 was delivered into VSMC by replication defective adenovirus DNA fragmentation was investigated by DNA Laddering and cell death ELISA VSMC in sub G1 was detected by using flow cytometry Results DNA from VSMC transfected by HCY2 gene was cleaved into multiple nucleosomes and VSMC in sub G1 region increased after being infected with HCY2 Conclusion HCY2 is able to induce apoptosis in VSMC

Objective To investigate the effect of nitric oxide synthase inhibitor on learning and memory abilities and to observe the change of nitric oxide (NO) contents in the hippocampi and the effect of nimodipine in rats.Methods With the administration of L ? nitro L arginine(L NA) into bilateral hippocami,the rat model with disorders of learning and memory abilities was established.Then the intraperitoneal injections of nimodipine were given, Y maze tests were performed, and the NO contents in the hippocampi were measured in turn.Results The experiment showed that the learning and memory abilities of model and intervention rats were worse than those of the control rats ( P

Object:To understand the situation of postpartum abuse and postpartum depression Method:This was a community-based face-to-face survey of a representative group of women who had a child aged 6 to 18 months in 32 communities of Tianjin, Liaoning, Henan and Shaanxi provinces between Nov 1, 2001 and February28, 2002 Totally 12044 subjects were found by stratified cluster sampling Results:The incidence of postpartum depression (according to result of Zung's self-rate depression scale for one week assessment during the interview) was 20 9%, while the rate of postpartum abuse was 8 3% Women often suffered abuses with many forms (physical, sexual and emotional maltreatment), and recurrently for a long time Among them, sexual abuse was the most frequent one Multiple logistic regression showed association between abuse and depression Conclusion:The incidences of postpartum depression and postpartum abuse are high Being abused has influence on depression

Objective To observe changes of hemodynamics and regional cerebral blood flow(rCBF) in patients with Leukoaraiosis(LA) and to investigate the pathogenesis of LA.Methods 67 patients with LA were examined by Transcranial Doppler(TCD). Regional cerebral blood flow was detected by SPECT in 19 of 67 patients.Results The medium blood flow velocities(Vm) of internal carotid artery system in LA(included mild, moderate and severe group) were significantly decreased compared with the controls( P