Folate is a water-soluble vitamin that cannot be synthesized by the body itself and must be obtained by external supplementation.There is substantial evidence that folate deficiency causes neural tube defects, partial birth defects, dementia, and certain types of cancer.However, excessive supplementation of folic acid(FA), the main form of supplementation at present, will increase the potential risk of certain diseases for oneself and future generations.Therefore, it is of great significance to explore new supplementation methods for folate supplementation in the population.This paper will discuss the progress of 5-methyltetrahydrofolate(5-MTHF)supplementation and the feasibility of replacing the chemical folic acid in terms of toxicology, bioavailability, vitamin B12 coverup, single nucleotide polymorphisms of folate metabolism-related enzyme genes, and fortification effect.

Objective To research the procedure for creating an animal model of mitral regurgitation by implanting a device through the apical artificial chordae tendineae, and to assess the stability and dependability of the device. Methods Twelve large white swines were employed in the experiments. Through a tiny hole in the apex of the heart, the artificial chordae tendineae of the mitral valve was inserted under the guidance of transcardiac ultrasonography. Before, immediately after, and one and three months after surgery, cardiac ultrasonography signs were noted. Results All models were successfully established. During the operation and the follow-up, no swines died. Immediately after surgery, the mitral valve experienced moderate regurgitation. Compared with preoperation, there was a variable increase in the amount of regurgitation and the values of heart diameters at a 3-month follow-up (P<0.05). Conclusion In off-pump, the technique of pulling the mitral valve leaflets with chordae tendineae implanted transapically under ultrasound guidance can stably and consistently create an animal model of mitral regurgitation.