1.Therapeutic effect of Tangzhi Decoction on Type 2 Diabetes Mellitus Complicated with Hyperlipidemia
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(04):-
Objective: To observe the therapeutic effect of Tangzhi decoction on type 2 diabetes mellitus (DM2) complicated with hyperlipidemia. Methods: One hundred and five cases of DM2 complicated with hyperlipidemia were randomly allocated to two groups: 32 cases treated with phenformin served as control group and 33 treated with phenformin and Tangzhi decoction as treatment group. The treatment course last 2 months. Results: The symptoms were improved, levels of fasting blood glucose (FBS), two-hour postprandial blood glucose (2 hPBG) and glycosylated hemoglobin (HbA1c), low-density lipid cholesterol (LDL-C), total cholesterol and triglycerides lowered and high-density lipid cholesterol (HDL-C) increased in treatment group. The total effective rate was 90.9%in treatment and 56.3 %in control group. The differences of the above indexes were significant in the two groups (P
2.Study on the Bacterial Endotoxin Test for Compound Diclofenas Sodium and Lidocaine Hydrochloride In-jection
China Pharmacy 1991;0(03):-
OBJECTIVE:To establish a bacterial endotoxin test for compound diclofenac sodium and lidocaine hydrochlo?ride injection(CDL).METHODS:The limitation of endotoxin in CDL was determined and a interference test was performed to determine the maximum non-interference concentration.RESULTS:CDL could interfere the detection of bacterial endotoxin,which could be overcome through300-fold dilution of the preparation.CONCLUSION:The bacterial endotixin test can be used to replace the detection of pyrogen in CDL.
3.Determination of the Concentration of Rhein in Plasma by RP-HPLC
China Pharmacy 1991;0(03):-
OBJECTIVE:To establish a RP-HPLC method for determining rhein in plasma.METHODS:Column:Spherisorb C18(4.0mm?250mm,5?m),Mobile phase:0.1% of phosphoric acid(pH 3.0)-methanol(30∶70,V/V),Flow rate:1.0ml/min;Detection wavelength:435nm.RESULTS:Rhein was completely separated and no interference was found.The peak areas of rhein within the range between 0.032~4.8?g/ml showed good linearity of the concentrations,r=0.9 990.The detection limit of rhein in plasma was 16ng/ml.The recovery rate was 92.09%~98.53%.The within-day RSD was 1.65%~6.68%,and between-day RSD was 4.93%~10.01%.CONCLUSION:This method is simple and sensitive,and can be used for analysing rhein-containing biological samples.
4.Analysis of Shuanghuanglian Injection Powder by HPLC-ESI-mass/mass spectrometry
Chinese Traditional Patent Medicine 1992;0(09):-
AIM: To analyse the main chemical constituents of Shuanghuanglian Injection Power(Flos lonicerae japanicae,Radix scutellariae and Fructus forsythiae) by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS: The sample solution was analysed by a Zorbax C_18 column with a gradient mobile phase comprised of acetonitrile and water.Electrospray ionization mass spectrometry detector was used,full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS: To analyse the mass spectrum of every liquid chromatography peaks,we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder,9 compounds were identified,including quinic acid,caffeic acid,three monocaffeoylquinic acids,dicaffeoylquinic acid,forsythin,forsythoside and baicalin. CONCLUSION: Quinic acid is identified from Shuanghuanglian Injection Power for the first time.High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.
7.Analysis for reason of negative HBeAg result in HBV DNA-positive patients
Chinese Journal of Clinical Laboratory Science 2006;0(05):-
Objective To explore the reason of negative HBeAg result detected by ELISA in the specimen with both positive HBV DNA and positive PreS1 in order to provide objective analysis of HBV replication.Methods Senty-six sera with negative HBeAg,but positive PreS1 and HBVDNA were included in this study.(1) HBeAg was detected again by ELISA after the specimen was diluted at a ratio of 1:100.(2) HBeAg/IC was detected by solid ELISA using monoclonal antibody against HBeAg.(3) The gene mutation of Pre C region was detected by oligonucleotide hybridization.Results HBeAg was positive in 5 specimen which were detected in dilution of 1:100 and HBeAg/IC was detectable in 38 specimen.The gene mutation of Pre C region was found in 24 specimen.Conclusion Hook effect of post-zone,formation of HBeAg/IC and gene mutation of Pre C region were the main reasons of negative HBeAg result by ELISA in the specimen with positive HBV DNA.The specimen with positive HBV DNA and negative HBeAg were presented in the patients who were mostly infected by wild type of HBV.Formation of HBeAg/IC result in the undetectable HBeAg in serum by routine ELISA.
8.Chemical constituents in fruits of Catalpa ovata
Chinese Traditional and Herbal Drugs 1994;0(01):-
Objective To study the chemical constituents in fruits of Catalpa ovata. Methods Isolation and purification were carried out by silica gel, Sephadex LH-20, and active carbon column chromatography etc. Constituents were identified and structurally elucidated by physicochemical properties and spectral analysis. Results Eight compounds were obtained, six of them were determined as catalpol (Ⅰ), catalposide (Ⅱ), ursolic acid (Ⅲ), ?-daucosterol (Ⅳ), nonacosane (Ⅴ), ?-sitosterol (Ⅵ). (Conclusion)(All (~1H-NMR) and (~(13)C-NMR) chemical shifts of the compound) Ⅱ are assigned by UV, IR, ESI-MS, HREI-MS, (~1H-NMR), (~(13)C-NMR), HMQC, and HMBC techniques. Compounds Ⅲ and Ⅴ are isolated from the plant for the first time.
9.A new benzofuran in Catalpa ovata
Chinese Traditional and Herbal Drugs 1994;0(02):-
Objective To study the chemical constituents in fruits of Catalpa ovata.Methods Various chromatographic techniques were used to separate and purify the constituents.Structures of the compounds were elucidated by physicochemical properties and spectral analysis (UV, IR, ESI-MS, 1H-NMR , 13 C-NMR , 1H- 1H COSY , HMQC, HMBC, NOESY, and CD).Results A compound was isolated and identified as 2(S)-(3′-hydroxy-5′-methoxy)-benz-3(S)-ethoxycarbonyl-6-trans-ethyl acrylate-8-methoxy-benzofuran.Conclusion This compound is a novel compound named as catalpafurxin.
10.Enhancement of systemic and CNS delivery of meptazinol hydrochloride by intranasal administration to rats
Zhenqi SHI ; Qizhi ZHANG ; Xinguo JIANG
Acta Pharmaceutica Sinica 2005;40(8):754-757
Aim To investigate the extent of systemic absorption and uptake of meptazinol (MEP) hydrochloride in cerebrospinal fluid (CSF) after intranasal administration on rats and compare with oral administration. Methods CSF samples were collected by a serial sampling method. The concentration of MEP in the biological samples was measured by HPLC with fluorescence detector. Results Rapid and significant levels of MEP in plasma and CSF can be achieved after nasal administration whereas the oral administration resulted in considerably lower drug concentrations. AUC in plasma and CSF from the nasal route are 7.375 and 15.6 folds compared with those of the oral route, respectively. Conclusion Intranasal MEP is able to show quick absorption and improve the bioavailability, which could be a promising alternative to oral administration.