OBJECTIVE:To establish a method for the content determination of L(-)-Anabasine in different medicinal parts (stem,rhizome,fibrous root,rootlet) of Alangium chinense,and provide reference for its development and utilization. METH-ODS:HPLC was performed on the column of Thermo C18 with mobile phase of methanol-phosphate buffer solution(22∶78,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 259 nm,column temperature was 30 ℃;the injection volume was 10 μl. RESULTS:The linear range of L(-)-Anabasine was 0.020 62-0.257 80μg(r=0. 999 9);the limit of quantitation was 1.7 ng,limit of detection was 0.5 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 97.38%-98.86%(RSD=0.6%,n=6). The content of L(-)-Anabasine in different medicinal parts was the fibrous root>the rootlet>the rhizome>the stem. CONCLUSIONS:The method is simple,accurate,reproducible and specific,and suitable for the content determination of L(-)-Anabasine in different medicinal parts(stem,rhizome,fibrous root,rootlet)of A. Chinense.

The publication of research papers is an important mark of achievements in scientific research. It is an index for checking the completion of various projects as well as a form of project completion. In order to better fulfill the tasks in scientific research, to enable more research projects to become bid winners, to turn out more quality papers, to produce outstanding results, and to further strengthen management and supervision of scientific research, the hospital the authors work with formulated at the end of 1999 "quantitative economic management of research quotas", which was put into effect at the beginning of 2000. An effect analysis of the implementation of the measure in the past three years showed that the desired results were basically achieved, academic advancement and personnel growth were greatly promoted, and the publication of research papers became a key index for evaluating the timely completion of research projects. In future the hospital plans to further improve the policy for rewarding writers of research papers and encourage quality academic papers to be published in key international journals.

In light of the spread and establishment of hospital information systems in the Shanghai area,the paper argues that government departments in charge ought to start in good time research on digitalized hospitals so as to better standardize how hospitals should promote in an active and steady manner a new round of development of hospital information systems.It also gives a detailed account of the content and methods of research on digitalized hospitals as well as development strategies in Shanghai.

Objective To evaluate suitable scan sequence of Low Field (0 35 T) MRI for children′s brain in development phase Methods The T 2 values of gray and white matter of centrum semiovale were measured The change of the T 2 value with the age was observed Three types of long TR scan sequence were performed These were routine TR, i e 2 700/40, 80 ms, long TR, i e 4 000/40, 80 ms, and extra long TR, i e 5 000～8 000/40, 80 ms They were applied to children of different age group And the quality of T 2WI was compared and analyzed Results The change of T 2 value was consistent with the process of myelinization Extra long TR sequence should be chosen for newborns Long TR sequence should be chosen for infants And routine TR sequence may be used in children older than one year old Conclusion In observing cerebral ventricle and extracerebral space of newborn and infant, image of long TR sequence is better than that of routine TR sequence

At present PLA and its copolymer is a kind of most widely used biodegradable polymers to prepare microspheres because of its good biocompatibility. The double emulsion method is the most used technique for microspheres loade with water-soluble drugs, proteins and peptides. Microspheres with different particle size or release character could be used in different applications such as targeted drug delivery or long-acting drug delivery. The characters of microspheres are influenced by the preparative parameter. This article reviewed the preparative parameters that influence the character of microspheres.

Objective To investigate changes of striatal DAT following hypoxic ischemic (HI)brain injury in newborn piglets using 11C-N-2-carbomethoxy-3-(4-fluorophenyl)-tropane (CFT) PET/CT,and to evaluate the value of 11C-CFT PET/CT in brain injury.Methods Newborn piglets with HI brain injury (n =20) were taken as a model group,and five piglets were used as a control group.Radioligand 11CCFT (55.5-74.0 MBq) was injected through the jugular vein,and PET/CT imaging was performed to observe the changes of striatal DAT in newborn piglets.The ST/occipital lobe (OC) ratio was calculated.Model group was divided into 0-6 h,20-24 h,44-48 h and 68-72 h sub-groups after HI in accordance with the imaging time.The piglets were sacrificed immediately after 11C-CFT PET/CT scanning,and then the brains were removed for pathological analysis.Data analysis was performed with one-way analysis of variance and Pearson linear correlation analysis.Results After intravenous injection of 11C-CFT,the radioactivity accumulation in cortical,striatum,and cerebellum was shown clearly in the control and model groups.The radioactivity accumulation was lower in the white matter.The radioactivity in cortical and cerebellum exhibited decreased with time,while the striatum was still clear.After HI,the ST/OC activity ratio in the striatum was initially increased,and the ratio of 0-6 h group (1.34 ± 0.04) was statistically significant compared with that of thecontrol group (1.18 ± 0.06 ; F =4.658,P ＜ 0.05),followed by a gradual decrease.ST/OC ratios of other HI subgroups were 1.27 ±0.01,1.27 ±0.10 and 1.18 ±0.05,respectively.There was a positive correlation between the number of DAT positive neurons ((13 ± 3),(13 ± 4),(8 ±3) and (4 ±4)/high power field) and 11C-CFT ST/OC activity ratios (r =0.844,P ＜0.05).Conclusion 11C-CFT PET/CT study can accurately reflect the changes of DAT in the striatum,and the amount of DAT is related to the severity of the ischemic insult in a newborn piglet model of HI.

Post-traumatic stress disorder (PTSD) is a mental disorder causing great distress to individuals, families and even society, and there is not yet effective way of unified prevention and treatment up till now. Lots of neuroimaging techniques, however, such as the magnetic resonance imaging, are widely used to the study of the pathogenesis of PTSD with the development of medical imaging. Functional magnetic resonance imaging (fMRI) can be applied to detect the abnormalities not only of the brain morphology but also of the function of various cerebral areas and neural circuit, and plays an important role in studying the pathogenesis of psychiatric diseases. In this paper, we mainly review the task-related and resting-state functional magnetic resonance imaging studies of the PTSD, and finally suggest possible directions for future research.
Brain ; physiopathology ; Humans ; Magnetic Resonance Imaging ; Neuroimaging ; Stress Disorders, Post-Traumatic ; physiopathology

Objective To report the MRI findings of brain damage obsenrved in neonatal patients who suffered from isolated hypoglycemia and to explore the value of diffusion-weighted imaging(DWI) inearly detection of neonatal hypoglycemic brain iniun,. Methods Twelve neonates with isolated hypoglycemia(10 of the 12 were diagnosed to suffer from hypoglycemic encephalopathy)were enrolled in this study.They were first scanned at age from 3 days to 10 days with Tl WI,T,WI and DWI(b is 0 s/mm2,1000 s/mm2),and 4 of them were then scanned from 7 days to 10 days following the initial scan.All acquired MR images were retrospectively analysed.Results First series of DWl images showed distinct hyperintense signal in 11 cases in several areas including bi lateral occipital cortex(2 cases),right occipital cortex(1 case),left occipital cortex and subcortical white matter(1 case),biIateral occipital cortex and flubcortical white matter(2 cases),bilateral parieto-occipital cortex(2 cases),bilateral parieto-occipital cortex and subcortical white matter(2 cases),the splenium of corpus catlosum(4 cases),bilateral corona radiata(2 cases),left eaudate nucleus and globus pallidus(1 case),bilateral thalamus(1 case),bilaterally posterior limb of internal capsule(1 ease).In the initial T1 WI and T2,WI images,there were subtle hypointensity in the damaged cortical areas(3 cases),hyperintensity in the bilaterally affected occipital cortex(1 case)on T1 weighted images,and hyperintensity in the affected cortex and subcortieal white matter with poor differentiation on T2 weighted images.The followed-up MRI of 4 cases showed regional encephalomalaeia in the affected occipital lobes(4 cases),slightly hyperintensity on T2 weighted images in the damaged occipital cortex(2 cases),extensive demyelination(1 case).disappearance of hyperintensity of the splenium of corpus callosum(1 case),and persistent hyperintensity in the splenium of corpus callosum (1 case)on T2 weighted images.Conclusion The findings suggest that posterior parieto-occipital regions are most frequently injured in neonatal period due to severe hypoglycemia.DWI is a useful technique in the early detection and evaluation of hypoglycemic brain injury of neonates.

Objective To compare the efficacy and side effects of intermittent androgen depriva-tion (IAD) versus continuous androgen deprivation (CAD) in prostate cancer. Methods Forty-four patients with prostate cancer were divided into 2 groups. Twenty-one cases (group IAD) received IAD therapy. Of them, TNM staging showed T2 in 7 cases, T3 in 9 cases, T4 in 5 cases. The patients were treated by maximum androgen blockage until the serum PSA decreased to less than 0.2 ng/ml and maintained for 2 months. The treatment was resumed when the serum PSA increased up to 10.0 ng/ml or the symptoms occurred progress. Twenty-three cases (group CAD) underwent CAD therapy. Of them, TNM staging showed T2 in 7 cases,T3 in 12 cases,T4 in 4 cases. The time to prostate cancer progression,quality of life and side effect rate were compared between the 2 groups. Results The median time to disease progression was (36±4) months in group IAD and (30±4) months in group CAD,respectively. There was no significant difference between the 2 groups (P=0.132). The mean cycle length was (15.9±2.3) months, among them time on treatment and time off treatment were(8.8±1.5) months and (7.3±0.8) months, respectively. The symptom scores related to treatment in the treatment period and intermission of IAD group were 55.9±16.8 and 47.9±19.7, respective-ly, there was significant difference between them(P=0.007). But the differences between the treat-ment period and intermission in bone pain,urinary and intestinal symptoms were not significant (P> 0.05). The urinary symptom scores after 5 months of continuing treatment in groug CAD was signifi-cantly higher than the basis reference value which was obtained in the sixth month of initial treatment(P=0. 023), but there was no significant changes in the scores of bone pain, intestinal symptoms and symptoms related to treatment(P>0.05). The incidences of hot flash and gynecomastia were 28.6% (6/21) and 19.0%(4/21) in group IAD, 60.9%(14/23) and 52.2%(12/23) in group CAD, respec-tively. There were significant differences between the 2 groups(P<0.05). Conclusions IAD thera-py can alleviate the side effects of androgen deprivation therapy and improve the life quality. The effica-cy of prolonging the time to androgen independence of IAD therapy is similar to CAD therapy.

Objective To investigate cortical activation patterns for covert and overt picture naming with functional magnetic resonance imaging (fMRI). Methods fMRI data were collected on 24-27 years old volunteers during performance of covert and overt picture naming. After statistical postprocessing analysis, head movement data were compared across tasks and average neural activation maps were available for both tasks. Results Mean and maximal translations of head movement in covert picture naming were less than those in overt picture naming, but the difference has no statistical significance (P=0.23). It was shown that covert picture naming involved an orchestration of bilateral occipital gyri and cerebellums, bilateral supplementary motor area, postcentral gyri, inferior frontal gyrus and anterior cingulate cortex. Activations in overt picture naming included those in covert naming (but more intensive), bilateral precentral gyri and posterior superior temporal gyri, left anterior superior temporal gyrus, bilateral thalamus, basal ganglia, and left insula. Conclusion Covert and overt picture naming are two different tasks involving different neural processing networks and levels. They can not be taken as substitutes for each other.