1.Treatment of 32 Cases of Psoriasis by Cotton Moxibustion
Journal of Acupuncture and Tuina Science 2004;2(1):14-15
32 cases of psoriasis were treated by cotton moxibustion. First, the dermal needle was used to tap the local skin lesion till slight bleeding. Then after an extreme thin layer of absorbent cotton was stuck on the skin lesion, the cotton was ignited with a match and then fire was flown off immediately.This procedure was repeated for 3 - 4 times. After treatment for 2 months, the results showed clinical cure in 23 cases, remarkable effect in 6 cases and improvement in 3 cases, indicating that cotton moxibustion treatment for psoriasis could speed up local blood circulation, improve micro-circulation of the skin, enhance the immune function of the human body and eliminate the papules and skin lesion.
2.Surgical report of 13 cases of penis lymphangioma.
Jianhong RONG ; He HUANG ; Xi QU ; Qixiang WANG ; Siwei ZHOU
National Journal of Andrology 2004;10(3):191-192
OBJECTIVETo study the diagnosis and surgical treatment of penis lymphangioma.
METHODSClinical data of 13 cases of penis lymphangioma were analysed restrospectively.
RESULTSThe 13 cases, treated by radical lymphangiomaectomy, were followed up for 2 to 3 years, and none of them had recurrence. This therapy affected neither the penile appearance, nor the erectile function of the patients. So their sexual life remained intact.
CONCLUSIONRadical ectomy should be the first choice of treatment for penis lymphangioma.
Adolescent ; Adult ; Child ; Humans ; Lymphangioma ; surgery ; Male ; Penile Neoplasms ; surgery ; Retrospective Studies
3.The distribution and structure of class Ⅰ integron in the multidrug-reisistant Pseudomonas aeruginosa
Jianguo CHEN ; Xiaoli DAI ; Yufeng JIANG ; Yingzhao LIU ; Jianren YU ; Zhaoliang SU ; Xinxiang HUANG ; Chiyu ZHANG ; Shengjun WANG ; Qixiang SHAO ; Shihe SHAO ; Huaxi XU
Chinese Journal of Laboratory Medicine 2008;31(8):872-875
Objective To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa (P. aeruginosa) isolated from Zhenjiang area to 13 routinely used antibiotics and identify the structure and dissemination of class Ⅰ integron. Methods K-B test was used to determine the resistant rate of 71 strains of P. aeruginosa. DNA template was extracted by boiling method, PCR method was utilized to detect class Ⅰintegron, and subsequently gene cassettes were analyzed by sequencing. Results The resistant rates to 13 routinely used antibiotics were quite different from 18. 3 to 77.5% among 71 strains of P. aeruginosa. The prevalence of class Ⅰ integron was 38%. These integrons include 5 gene cassettes ( aadB, aac (6) - Ⅱ , PSE-Ⅰ , dfrA17 and aadAS), in which dfrA17 and aadA5 gene cassette were frequently found. Comparing with the negative strains of integron, the positive strains of integron has obviously higher resistance to ten the antibiotics including piporacillin, piperacillin-tazobactam, ceftriaxone, cefepime, ceftazidime, gentamicin,amikacin, tobmmycin, levofloxacin, and ciprofloxacin. Conclusions The resistant rates of P. aeruginosa to 13 drugs were different, and the resistant rates of integron positive strains were obviously higher than integron negative strains, which indicates that integron may play an important role in multidrug reisistance of P. aeruginoosa.
4.Reliability and validity of the Chinese version of International Consultation on Incontinence Modular Questionnaire for Male Lower Urinary Tract Symptoms
Weina HUANG ; Peng WU ; Yaoguang ZHANG ; Qixiang SONG ; Qiang WANG ; Dalei ZHANG ; Jianye WANG
Chinese Journal of Urology 2019;40(4):299-303
Objective To translate the International Consultation on Incontinence Modular Questionnaire for Male Lower Urinary Tract Symptoms (ICIQ-MLUTS) and ICIQ-MLUTS long form (ICIQ-MLUTS LF) into Chinese and assess their metric properties and applicability.Methods After obtaining permission from the International Consultation on Incontinence Questionnaire (ICIQ),original ICIQ-MLUTS and ICIQ-MLUTS LF were translated into Chinese according to ICIQ validation protocol and cultural adaptation guideline.During November 2017 to August 2018,Chinese version of ICIQ-MLUTS and ICIQ-MLUTS LF were tested by administering them in 6 and 12 hospitals in China respectively.To validate the translated questionnaires,the following tests were undertaken.The content validity was determined by indepth interviews with participants and experts.The correlation coefficients of ICIQ-MLUTS and ICIQ-MLUTS LF with International Prostate Symptom Score (IPSS) were assessed to validate criterion validity.Cronbach's alpha test was used to explore internal consistency.And the test-retest reliability was evaluated by calculation of intraclass correlation coefficient.Results In total,Chinese ICIQ-MLUTS and ICIQ-MLUTS LF were administrated to 135 and 230 male patients with lower urinary tract symptoms respectively.Both questionnaires had good content validity and good criterion validity with IPSS (Pearson correlation 0.846 and 0.833 for ICIQ-MLUTS and ICIQ-MLUTS LF respectively,both P < 0.001).The Cronbach's alpha coefficient was 0.797 for ICIQ-MLUTS,and 0.853 for ICIQ-MLUTS LF.Intraclass correlation coefficient was 0.986 and 0.985 respectively (both P < 0.001),showing good test-retest reliability.Conclusions The Chinese version of ICIQ-MLUTS and ICIQ-MLUTS LF had good validity and reliability,which can be used to assess Chinese male patients with lower urinary tract symptoms.
5.Effect of Lactobacillus plantarum WCFS1 on pancreatic and ileal injury in mice with acute necrotizing pancreatitis
Binqiang XU ; Wenfei QIN ; Yang FU ; Nuoming YIN ; Zehua HUANG ; Qixiang MEI ; Chunlan HUANG ; Yue ZENG
Chinese Journal of Pancreatology 2023;23(2):121-127
Objective:To explore the effect of probiotics Lactiplantibacillus plantarum(LP) WCFS1 by gavage on acute necrotizing pancreatitis (ANP) and associated ileum injury in mice. Methods:Twenty-four healthy male mice were gavaged with broad-spectrum antibiotics for 3 weeks to establish microbiota-depleted mice, and then randomly divided into control group (CON), ANP model group (ANP), LP gavage group (LP) and LP gavage and ANP induced group (LP+ ANP) , with 6 mice in each group. Mice in LP and LP+ ANP group were treated by gavage of LP (1×10 9 CFU/ml, 0.2 ml/day per mouse) for 1 week, while CON and ANP were gavaged with sterile phosphate buffered saline for 1 week instead. The ANP model was induced by intraperitoneal injection with caerulein (100 μg/kg) for 10 times with 1-hour interval between two injections and the 10th injection with lipopolysaccharide(LPS) 5 mg/kg intraperitoneally, and the mice were sacrificed 2 h later. Levels of LP in stool and ileal mucosa were detected by real-time PCR; the pancreas and ileum were collected for pathological examination to observe the extent of tissue inflammation and to score the pathology. Serum amylase activities were determined by enzymatic kinetic chemistry; serum inflammators levels and intestinal permeability were detected by ELISA; levels of inflammators in pancreatic and ileal tissues were detected by real-time PCR; ileal tight-junction proteins (occludin, claudin-1 and ZO-1) were measured by immunofluorescence staining. Results:LP levels in the stool and ileal mucosa of mice were significantly increased after LP gavage, and the differences were statistically significant (913.30±39.12 vs 2.39±1.39, 23.11±0.50 vs 1.38±0.28, all P value <0.05). The pathological scores of pancreatic tissue of CON, LP, ANP and LP+ ANP group were (0.26±0.41), (0.17±0.26), (8.55±0.46) and (6.30±0.45); the serum amylase activities were (219.70±19.73), (217.60±11.30), (2896.24±98.32) and (1837.13±131.60)U/L, IL-1β were (0.87±0.28), (1.4±0.85), (67.41±6.45) and (36.33±5.65)pg/ml, IL-6 were (0.74±0.27), (0.16±0.16), (280586.12±39163.92) and (107912.75±31283.47)pg/ml, IL-10 were (35.52±5.27), (50.99±15.34), (2008.45±184.83) and (3070.35±403.71)pg/ml; the expression level of pancreatic IL-1β mRNA was 1.42±0.39, 0.95±25, 20.53±0.50 and 10.69±1.01, IL-6 mRNA was 1.31±0.44, 0.93±0.023, 21.97±1.71 and 11.54±1.75, IL-10 mRNA was 0.93±0.14, 0.75±0.15, 0.99±0.21 and 1.76±0.19; there was no significant difference between LP and CON group, and pancreatic pathological scores, serum amylase、IL-1β and IL-6 levels, and the expression level of pancreatic IL-1β and IL-6 mRNA were significantly decreased in LP+ ANP group compared with those in ANP group, while serum IL-10 levels and the expression level of pancreatic IL-10 mRNA were significantly increased compared with ANP group, and all the differences were statistically significant (all P values <0.05). The pathological scores of ileal tissue of CON, LP, ANP and LP+ ANP group were 0, 0, (3.17±0.41) and (1.67±0.52); the levels of serum DAO of CON, LP, ANP and LP+ ANP group were (0.03±0.03), (0.02±0.02), (0.50±0.05) and (0.49±0.06)ng/ml; LPS levels were (2.75±0.35), (3.74±0.28), (7.19±0.92) and (5.88±0.38)ng/ml; the expression level of ileal IL-1β mRNA was 1.21±0.20, 1.17±0.09, 1.81±0.25 and 1.63±0.21; IL-6 mRNA was 1.01±0.29, 2.83±0.42, 54.45±8.50 and 16.87±4.42; IL-10 mRNA was 1.12±0.41, 6.09±2.51, 11.65±1.47 and 29.86±2.93. There was no significant difference between LP and CON group, except that the ileal IL-10 mRNA expression was significantly higher than that of CON group. Ileal pathological scores, serum LPS levels and the expression level of ileal IL-6 mRNA were significantly lower in LP+ ANP group than those in ANP group, while the expression level of ileal IL-10 mRNA was significantly higher than that of ANP group; the expression of ileal tight junction proteins (ocludin, claudin-1, ZO-1) was significantly higher than those in ANP group, and all the differences were statistically significant (all P values <0.05). Conclusions:LP WCFS1 gavage could ameliorate the injury of pancreatic and ileal barrier in caerulein-induced ANP mice.
6.Adeno-associated virus mediated T-bet gene transfer into SGC-7901 cell to regulate IFN-gamma production.
Gufeng QIU ; Suoying WANG ; Shengjun WANG ; Qixiang SHAO ; Jie MA ; Ming YANG ; Xiaopeng XU ; Chaoming MAO ; Zhaoliang SU ; Xinxiang HUANG ; Huaxi XU
Journal of Biomedical Engineering 2009;26(3):606-619
In order to investigate the effect of T-bet on malignant cells, we selected SGC-7901, a kind of human gastric carcinoma cell line, and used gene clone technique and adeno-associated virus (AAV) packing technology, thus obtaining a recombinant rAAV-eGFP-T-bet and T-bet gene-transfected SGC-7901 cells. Then the function of T-bet gene-infected SGC-7901 cells was researched by detecting the levels of IFN-gamma and T-bet production. The results showed: (1) It was verified that rAAV-T-bet's packing was completed; (2) After SGC-7901 cells was transfected by rAAV-eGFP-T-bet, a green fluorescence was found in about 30%-40% SGC-7901s, and the gene of 1670 bp (T-bet) and 388 bp (IFN-gamma) were generated from SGC-7901s cells; (3) The proteins of IFN-gamma and T-bet secreted by SGC-7901 cells were also detected. These reveal that SGC-7901 cell is efficiently infected by rAAV encoding T-bet, which can induce transfected cells to secret IFN-gamma. It may be useful in the researches on cancer immune therapy of transfecting T-bet gene.
Cell Line, Tumor
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Dependovirus
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genetics
;
metabolism
;
Green Fluorescent Proteins
;
biosynthesis
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Humans
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Interferon-gamma
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biosynthesis
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Recombinant Proteins
;
biosynthesis
;
genetics
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Stomach Neoplasms
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genetics
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metabolism
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T-Box Domain Proteins
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biosynthesis
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genetics
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Transfection
7.Application of 3.5 mm superfine bronchoscope in children′s difficult airway
Xinlin DENG ; Jiancheng HUANG ; Tieliang CAI ; Qixiang SHEN ; Hongqiang HU
Chinese Journal of Postgraduates of Medicine 2018;41(1):42-45
Objective To discuss the practicability and safety of 3.5 mm superfine bronchoscope in children's difficult airway.Methods Twenty-eight children with difficult airway,aged 4 months to 8 years were selected.Intravenous anesthesia was used and autonomous respiration was maintained.The 3.5 mm superfine bronchoscope was used to guide tracheal intubation by nose(mouth).Results Among 28 cases, 1 case had oral bleeding and succeeded after suction and pressurized oxygen supply, and the rest was successful in the first time.The intubation time was(1.50 ± 0.25)min.There was no damage to the whole airway.Success rate was 100%.Conclusions The 3.5 mm superfine bronchoscope in guiding tracheal intubation of children's difficult airway has obvious advantages and security, and it deserves clinical attention and promotion.
8.Survey and Consideration on the Current Situation of Service Ability and Pharmaceutical Service Construction of Primary Healthcare Institutions in Jiangsu Province
Qixiang HUANG ; Mengqing LU ; Ailin XU ; Xiaoyu XI
China Pharmacy 2019;30(23):3287-3292
OBJECTIVE: To explore the current situation of service ability and pharmaceutical service construction of primary healthcare institutions in Jiangsu province. METHODS: Questionnaire survey was conducted among primary healthcare institutions of 13 prefecture level cities in Jiangsu province, involving basic service information (such as serving mode, medical departments), resource allocation (such as hardware, human resource) and pharmaceutical care construction (such as the leaders of the drug procurement, pharmaceutical departments allocation, mediation safety management). The current situation of service ability and pharmaceutical service construction was evaluated in primary healthcare institutions of Jiangsu province. RESULTS: A total of 579 primary healthcare institutions in Jiangsu province were investigated and 579 questionnaires were collected (some items in the questionnaires were not answered). Outpatient service (543/555,97.8%) was the main mode of service provided by primary healthcare institutions in Jiangsu province, followed by emergency call (321/555,57.8%) and hospitalization (292/555,52.6%); the frequently established department in primary healthcare institutions was the medical department for all (452/568,79.6%), preventive healthcare department(401/568,70.6%), internal medicine department (365/568,64.3%); 16.1% (93/533)of the primary healthcare institutions had no beds; 36.4% (211/579)of the institutions had no chief physician; 20.9% (111/532)of the primary healthcare institutions medicine procurement were managed by pharmacists; 42.5% (212/499)of the institutions had no pharmaceutical departments; physicians were responsible for patients' medication safety in 43.9% (236/537) of the primary healthcare institutions and pharmacists were responsible for patients' medication safety in only 16.2% (87/537)of the institutions. CONCLUSIONS: Primary healthcare institutions in Jiangsu province have made some achievements in service capability building. Human resources, equipment and facilities, pharmaceutical care construction and other aspects still need to be improved.
9.Transcriptome sequencing of transgelin-2 inhibiting high glucose induced microglia inflammation
Pingling SHI ; Yuanmeng WEI ; Zixu HUANG ; Cong LU ; Qixiang YANG ; Pan LI ; Chengye WU ; Zongming SONG
Chinese Journal of Ocular Fundus Diseases 2023;39(2):153-162
Objective:To analyze the change of differential genes and signaling pathways in high glucose induced BV2 cells, and to explore the mechanism of transgelin-2 (TAGLN2) regulating cellular inflammatory response and metabolic process.Methods:An experimental study. The cultured BV2 cells were divided into mannitol treatment (Man) group, glucose treatment (Glu) group, overexpression control Glu treatment (Con) group, overexpression TAGLN2 Glu treatment group, silence control Glu treatment (shCon Glu) group, and silence TAGLN2 Glu treatment (shTAGLN2 Glu) group. Cells in the Man group were cultured in modified Eagle high glucose medium (DMEM) containing 25 mmol/L mannitol and 25 mmol/L glucose, cells in other groups (Glu group, Con Glu group, TAGLN2 Glu group, shCon Glu group and shTAGLN2 Glu group) were cultured in DMEM medium containing 50 mmol/L glucose. After 24 hours of cells culture, transcriptome sequencing of cells in each group were performed using high-throughput sequencing technology, and significantly differentially expressed genes (DEG) were screened. |log 2 (fold change)|≥1 and P≤0.05 were adopted as criteria to screen for DEG. Gene Ontology (GO) function enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and protein-protein interaction network analysis were performed. Real-time polymerase chain reaction (RT-PCR) was used to detect the relative expression level of DEG mRNA. The data between groups were compared by independent sample t-test. Results:When compared with Man group, a total of 517 differentially expressed genes were screened in Glu group, which including 277 up-regulated genes and 240 down-regulated genes. KEGG pathway enrichment analysis showed that the up-regulated genes were significantly enriched in immune system processes such as nuclear factor (NF)-κB signal pathway, Jak-signal transducers and activators of transcription (STAT) signal pathway, while down-regulated genes were significantly enriched in glycosaminoglycan degradation and glyceride metabolic pathway. Compared with Con Glu group, a total of 480 DEG were screened in TAGLN2 Glu group, among which 147 up-regulated and 333 down-regulated genes were detected. Up-regulated genes were significantly enriched in the metabolic processes of fatty acid, glyceride and pyruvate, while down-regulated genes were significantly enriched in immune system processes such as NF-κB signal pathway, Jak-STAT signal pathway and tumor necrosis factor (TNF) signal pathway. Compared with shCon Glu group, a total of 582 DEG were screened in shTAGLN2 Glu group, among which 423 up-regulated and 159 down-regulated genes were detected. Up-regulated DEG were significantly enriched in immune system processes such as TNF signal pathway and chemokine signal pathway, while down-regulated DEG were significantly enriched in pattern recognition receptor signal pathway. RT-PCR results showed that the relative expression levels of DEG mRNA Card11 ( t=13.530), Icos ( t=3.482), Chst3 ( t=6.949), Kynu ( t=5.399), interleukin (IL)-1β ( t=2.960), TNF-α ( t=5.800), IL-6 ( t=3.130), interferon-γ ( t=7.690) and IL-17 ( t=6.530) in the TAGLN2 Glu treatment group were decreased significantly compared with Con Glu group, and the difference was statistically significant. Conclusion:TAGLN2 can inhibit glucose induced microglia inflammation by NF-κB and Jak-STAT signaling pathways, Card11, Icos, Chst3 and Kynu play an important role in the anti-inflammatory process of TAGLN2.
10.Therapeutic effects and influencing factors of transurethral resection of bladder tumor in the treatment of cystitis glandularis
Jian LI ; Weilin FANG ; Qixiang SONG ; Xin SONG ; Tingting LYU ; Jin HUANG ; Xiang JI ; Zhikang CAI ; Zhong WANG ; Jianwei LYU
Journal of Modern Urology 2024;29(8):719-722
【Objective】 To evaluate the efficacy of transurethral resection of bladder tumor (TURBT) in treating cystitis glandularis (CG), and to explore the influencing factors. 【Methods】 A retrospective analysis was conducted on 243 CG patients treated with TURBT during Jan.2013 and Dec.2020 in our hospitals.Postoperative efficacy was assessed using global response assessment (GRA).The correlation between GRA score and the demographic characteristics, comorbidities, initial complaints, and postoperative recurrence was determined with logistic regression analysis. 【Results】 Among the 243 patients, 3.70% (9/243) had dysplasia, 2.47% (6/243) had exuberant hyperplasia of Brinell’s nest, and 2.06%(5/243) had intestinal metaplasia.The mean GRA score was (2.02±0.72) after a follow-up of (47.10±28.53) months.Re-operation was performed in 10.29% (25/243) of the patients due to recurrence, and the improvement of hydronephrosis and dysuria was 70.59% (12/17) and 50.00% (15/30), respectively.Pelvic fat increase developed in 1 patient (0.41%) after surgery.Logistic regression analysis showed that postoperative GRA score was not significantly correlated with demographic characteristics, body mass index, comorbidities, alcoholism and postoperative recurrence (P>0.05). 【Conclusion】 TURBT is an effective method in the treatment of CG, which can significantly improve patients’ hydronephrosis and dysuria.However, approximately 10% of the patients experience recurrence, necessitating further surgery, which suggests the need for vigilance regarding potential recurrence during treatment.