Objective To investigate the change of the bile acid in the blood serum and its significance in the neonates with hyperbilirubinemia.Methods The levels of total bile acid (TBA),the cytomegalovirus IGM and the liver function including the AST,the ALT and the GGT were detected in 298 neonates with hyperbilirubinemia.The neonates were divided into two groups by the levels of total bilirubin in the blood serum the light-degree and severedegree group,and which need to compare with the control group consisting of 29 neonates.Results There were 254neonates in the light-degree group and 44 in the severe-degree group.Making comparison of the average value including AST,ALT,GGT,TBA and the positive rate,it showed that there was no statistical significance between the two groups (P ＞ 0.05).The GGT and TBA in both the light-and severe-degree group were higher than the control group,the difference had statistical significance(P ＜0.01).Testing the CMV-IgM of 214 neonates with hyperbilirubinemia,it showed that the results were all normal.Conclusion Most neonates with hyperbilirubinemia suffer from liver parenchyma injury and cholestasis,also the levels of TBA in the blood serum have no concern with the degree of jaundice.So the liver protection drugs can be properly used in the treatment.The TBA is more sensitive than the ALT to diagnose the liver parenchyma injury in neonates with hyperbilirubinemia.

Objective To analyze the change of blood sugar in asphyxial neonates. Methods To test the blood sugar in 225 cases of asphyxial neonates and 43 cases of control group. Results There were no significant differences in the blood sugar between the asphyxial group and the control group[(3.85±2.01) vs (4.3±1.01), P > 0.05]. The quantity of blood sugar and the incidence rate of hyperglycosemia in seriously asphyxial group were more greater than slightly asphyxial group(P <0.01). The quantity of blood sugar in slighdy asphyxial group was less than the control group (P < 0.05). The quantiy of blood sugar in asphyxial premature infants was significantly less than asphyxial full-term infants(P < 0.05). Conclusions The blood sugar level in asphyxial neonates is relative to the degree of asphyxia and gestational age. The blood sugar in highly asphyxial neonates rise whereas in slightly as-phyxial neonates and asphyxial premature infants reduce.

Objective To investigate the changes and clinical significance of routine blood test in infants with rotavirus enteritis.Methods The white blood cell count (WBC),the platelet parameters (PLT),the platelet volume (MPV)and the platelet distribution width (PDW)were detected by automatic blood cell analyzer respectively in 314 infants with rotavirus enteritis before and after the therapy for 5 -7 days.The C -reactive protein (CRP)was also detected,then made comparison with the normal control group (40 cases).Results The WBC (10.77 ±4.20)× 109 /L,PLT(330.02 ±117.70)×109 /L,MPV(8.46 ±2.15)fL in the infants with rotavirus enteritis were higher than the WBC(7.69 ±2.06)×109 /L,PLT(298.45 ±57.19)×109 /L and MPV(7.52 ±0.85)fL in the control group before the therapy.The differences were statistically significant (t value =4.569,1.670,2.746,P =0.000, 0.000 and 0.047).The WBC(7.76 ±2.83)×109 /L and CRP(5.18 ±5.82)mg/L in the infants with rotavirus enteritis after the therapy were lower than the WBC(10.77 ±4.20)×109 /L and CRP(7.70 ±11.28)mg/L before the therapy(t value =9.794 and 2.810,P =0.000).Conclusion The WBC,PLT and CRP in infants with rotavirus enteritis were usually higher.But the raise of WBC and CRP could not be the only indication of using antibiotic in infants suffered from diarrhea.

Objective To investigate the changes and significance of platelet parameters and leucocyte in the children suffered from mycoplasma pneumoniae pneumonia (MPP).Methods The platelet parameters (PLT),the platelet volume(MPV),the platelet distribution width (PDW),and the white blood cell count(WBC) were detected by automatic blood cell analyzer respectively in 300 children with MPP before and after the treatment by macrolides antibiotics for a week as well as in 40 normal children.The comparison was made among them.Results There were 156 cases that suffered from thrombocytosis (PLT ＞ 300 × 109/L) in MPP group,the incidence rate was 52.0％(156/300).While there was 1 case with thrombocytopenia,the incidence rate was 0.3％ (1/300).The WBC,MPV and PDW in MPP group before and after one-week treatment were higher than the control group.And the PLT was higher than the initial stage of hospitalization and the control group.The differences had statistical significance.Conclusion The incidence rate of thrombocytosis was high in the children suffered from MPP.The dynamic observation of platelet parameters and WBC in children with MPP could be used as the elementary basis of judging MP infection.

Objective To analyse the change of the blood serum electrolyte in asphyxial neonates.Methods To test the concentration of blood serum K~+、Na~+、Cl~-、Ca~(2+) in enzyme linked immunosorbhent assay.In 343 cases,there were 297 cases of slight asphyxial neonates and 46 eases of serious neonates.In control group,there were 41 cases.Results The concentration of blood serum K~+、C~l-、Ca~(2+) in asphyxial neonates were significamly less than control group(all P<0.01) and the concentration after birth 24h～48h were significantly less than within 24h(all P<0.01).The incidence of hyponatremia,hypochloremia and hypocalcemia after birth 24h～48h were significantly hisher than within 24h(all P<0.01),There were no significant differences in the concentration of blood serum K~+、Na~+、Cl~-、Ca~(2+) between the slight asphyxial group and the serious asphyxial group(P>0.05).Conclusions The concentration of blood serum K~+、Na~+、Cl~-、Ca~(2+) in asphyxial neonates gradually reduced after birth 48h and tbe incidence of electrolyte disturbance gradually increased.The change of blood electrolyte should be dynamically routinely monitored.

Objective To investigate the changes and clinical significance of myocardial enzyme spectrum in the premature in-fants with hyperbilirubinemia .Methods The myocardial enzymes spectrum (AST ,CK ,CK-MB) in 90 premature infants with hy-perbilirubinemia were tested and the detection results were analyzed .Then their comparison with the control group consisting of 27 premature infants was performed .Results The detection results of myocardial enzymes spectrum in the hyperbilirubinemia prema-ture infants were higher than those in the control group ,but only the increase of CK-MB had statistical significance (P<0 .05);the positive incidence rate of the myocardial enzymes in the hyperbilirubinemia premature infants was 55 .56% (50/90) ,which was ob-viously higher than 7 .41% (2/27) in the control group ,the difference had statistical significance (P<0 .01) .Conclusion Hyperbil-irubinemia in premature infants can cause the myocardial damage and has the high incidence rate .The myocardial enzyme spectrum should be routinely detected for guiding the clinical treatment in the premature infants with hyperbilirubinemia .

Objective To investigate the changes of the anion gap(AG)in the premature suffered from hyperbilirubinemia,to provide the basis for treatment.Methods The serum electrolyte,the renal function and the liver function in 94 premature babies suffered from hyperbilirubinemia were tested,and then calculated the value of AG,while the arterial blood gas analyses were done in 36 cases.Results 44 cases had high AG(46.81％),44 cases had normal AG(46.81％)and 6 cases had low AG(6.38％).The increase of AG was directly proportional to the concentration of Na+in blood,while was inversely proportion to the concentration of HCO3-.There was no close relationship between the AG and BUN,Scr in the serum.Conclusion The incidence rate of metabolic acidosis poisoning was high in premature suffered from hyperbilirubinemia with high AG,so the routine test of blood electrolyte,renal function and arterial blood gas should be done.Calculate the value of AG to guide the treatment,and to avoid correcting the acid by the alkali blindly.

Objective To detect the damage incidence rate of liver and myocardium in infantile rotavirus (RV) enteritis.And to provide the basis for prevention and cure of liver and myocardium damage in infantile RV enteritis.Methods The liver function and myocardial enzyme detection results of 395 patients with infantile RV enteritis were collected .These results were compared with those of 40 healthy kids from health examinations . Results The levels of alanine aminotransferase ( ALT ) , aspartate amino transferase ( AST ) , serum creatine kinase (CK) and serum creatine kinase isozyme(CK-MB) from infantile patients were (34.49 ±29.13)U/L,(52.44 ± 24.10)U/L,(141.75 ±132.22)U/L and (48.69 ±32.53)U/L,respectively,which were higher than those of the healthy control [(16.00 ±3.24)U/L,(29.90 ±3.76)U/L,(101.82 ±64.56)U/L and (22.32 ±8.98)U/L,t=4.008,5.901,3.982,5.64,all P<0.05].The abnormal occurrence rates of ALT ,AST,CK and CK-MB in infantile patients were 19.49％,73.16％,12.15％,73.16％,respectively,which were higher than those in healthy controls (2.50％,20.00％,0.00％,0.00％),the differences were statistically significant (χ2 =7.128,47.397,5.464, 300.239,all P<0.05).The incidence rates of liver and myocardium damage were 19.49％and 73.16％,respectively. Conclusion There are higher incidence rates of liver and myocardium damage in infantile RV enteritis .So for the infantile RV enteritis patients ,it is necessary to detect the liver function and myocardial enzyme level .The treatment for infantile RV enteritis should be included liver and myocardial protection except antivirus and correcting dehydration .

OBJECTIVETo investigate the effects of combination therapy with methylprednisolone (MP) and brain-derived neurotrophic factor (BDNF) on axonal remyelination and functional recovery after spinal cord injury in rats.

METHODSForty-five rats were randomly divided into three groups: Group A received MP and BDNF; group B received MP and cerebrospinal fluid (CSF); and group C received CSF only. Contusion injury to adult rat spinal cord was produced at the T(10) vertebra level followed by immediate intravenous MP or CSF, and was thereafter infused intrathecally with BDNF or CSF for 6 weeks. Axonal remyelination and functional recovery was observed using RT-PCR, immunohistochemistry and open field locomotion.

RESULTSAn increase of 28.4% +/- 2.3% in the expression of proteolipid protein (PLP) gene, an endogenous indicator of axonal remyelination, was demonstrated in group A 24 hours after injury. Ten weeks later, there were significant decreases in hematogenous inflammatory cellular infiltration in groups A and B compared to C (P < 0.05). Concomitantly, a significant amount of axonal remyelination was observed in group A compared to groups B and C (P < 0.05). Furthermore, combination therapy using MP and BDNF in group A resulted in stimulation of hindlimb activity as well as improvement in the rate of functional recovery in open field locomotion (P < 0.05).

CONCLUSIONSCombined therapy of MP and BDNF can improve functional recovery through mechanisms that include attenuating inflammatory cellular infiltration and enhancing axonal remyelination at the injury site. Such a combination may be an effective approach for treatment of spinal cord injury.

Animals ; Axons ; physiology ; Brain-Derived Neurotrophic Factor ; administration & dosage ; Drug Therapy, Combination ; Female ; Methylprednisolone ; administration & dosage ; Myelin Proteolipid Protein ; genetics ; Nerve Regeneration ; drug effects ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley ; Recovery of Function ; Spinal Cord Injuries ; drug therapy ; metabolism ; physiopathology

Objective:To retrospectively analyze the urodynamics quality in Southwest China, and find out the main issues of urodynamics quality in Southwest China and try to find out the improvement ways.Methods:In this study, a two-stage sampling method was used.In the first stage, 10 medical institutions in Southwest China were selected by cluster sampling from March to June, 2020.In the second stage, according to the development of UDS in Southwest China, the sample size estimation formula was adopted, and the loss of follow-up rate in reports extraction was considered, the initial sample size was 350. As the workload of UDS in the 10 medical institutions involved in the study was equivalent, 35 urodynamics traces from each medical institution were selected. The initial samples should also meet the inclusion criteria: ①patients with clear medical history and complete clinical data; ②UDS traces were clear; ③UDS system was water filled system; ④age>18, and 150 urodynamic traces were included in the final study. We evaluated the quality of enrolled urodynamics traces, and the quality evaluation standard according to the guidelines established by the International Continence Society (ICS). The evaluation conducted by two independent urologist with more than 10 years working experience. Artifacts were divided into non-technical artifacts: abnormal abdominal pressure changes, urine volume <150 ml when did the uroflow test, and technical artifacts: non-standard zero setting, fail to record all urodynamics parameters, baseline drift, catheter displacement, misjudgment of detrusor physiological contraction and detrusor overactive in voiding phase, misjudgment between detrusor overactive and bladder low compliance in filling phase.Results:non-technical artifacts: 32 cases were found abnormal abdominal pressure changes (21.3%), 21 cases (14.0%) were found when did the uroflow test the urine volume <150 ml, and technical artifacts: Non-standard zero setting in 28 cases (18.7%), fail to record all urodynamics parameters in 8 cases, baseline drift in 16 cases, catheter displacement in 9 cases and misjudgment of detrusor physiological contraction and detrusor overactive in voiding phase in 12 cases, misjudgment between detrusor overactive and bladder low compliance in filling phase in 24 cases (16.0%).Conclusions:At present, the urodynamics quality in Southwest China need to be improved. The main issues were that the operator didn’t obey the basic operation and quality control process, and the operator did not have enough basic knowledge of urodynamics. It can be improved by strictly carry out the operation standard of UDS, identifying and correcting artifacts in time, and promoting the standardized urodynamic training courses.