OBJECTIVETo investigate gene expression profile in nasopharyngeaL carcinoma (NPC) cell lines with different metastatic potentialities, in order to identify new candidate genes related to the development, progress and metastasis of NPC.

METHODSThe mRNA expressions of high metastatic NPC cell line 5-8F, tumorigenic but nonmetastatic NPC cell line 6-10B and non-tumorigenic NPC cell line 13-9B (3 sublines of SUNE-1) were investigated by cDNA microarray containing 14 000 cDNA clones. The alterations in gene expression levels were confirmed by reverse-transcription PCR.

RESULTSThere were 82 differentially expressed genes comparing 5-8F and 13-9B; 38 differentially expressed genes comparing 6-10B and 13-9B; 54 comparing 5-8F and 6-10B. There were 12 common differentially expressed genes comparing 6-10B, 5-8F and 13-9B; 14 common differentially expressed genes comparing 5-8F and 13-9B, 6-10B. The expressions of the above genes were involved in metabolism, transcription, differentiation, apoptosis and signal transduction.

CONCLUSIONThe gene expression profile in nasopharyngeal carcinoma cell lines is an important index in the search of new candidate genes related to NPC.

Cell Line ; DNA, Complementary ; analysis ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Neoplasm Metastasis ; genetics ; Oligonucleotide Array Sequence Analysis ; Tumor Cells, Cultured

Objective:To explore the effects of peer support-based rehabilitation on symptom burden and resilience of lung cancer patients undergoing chemotherapy, so as to provide reference for relieving the symptoms of patients during chemotherapy.Methods:A total of 90 lung cancer patients who undergoing chemotherapy in Nanjing First Hospital, Nanjing Medical University were assigned to the experimental group and the control group according to the enrolled time, with 45 cases in each group. The patients from January to November 2018 in the control group recieved routine chemotherapy care and rehabilitation, the patients from January to November 2019 in the experimental group implemented peer support-based rehabilitation based on the routine chemotherapy care. The symptom burden and resilience on admission and discharged from hospital were assessed by Chinese version of the MD Anderson Symptom Inventory (MDASI-C) and Connor-Davidson Resilience Scale (CD-RISC), respectively.Results:There was no significant difference in the scores of MDASI-C, CD-RISC on admission between the two groups( P>0.05). At discharge, the symptom distress scores and life influence scores in MDASI-C were (6.19 ± 1.72), (5.72 ± 1.45) points in the experimental group, significantly lower than those in the control group (7.23 ± 2.02), (6.75 ± 1.67) points; the tenacity, power, optimism dementions socres and total scores of CD-RISC were (28.12 ± 4.33), (20.88 ± 5.64), (10.69 ± 3.00), (59.69 ± 8.25) points in the experimental group, significantly higher than those in the control group (24.57 ± 4.14), (17.83 ± 4.09), (9.39 ± 2.58), (51.82 ± 5.84) points. The differences were statistically significant ( t values were 2.18-5.13, all P<0.05). Conclusions:Peer support-based rehabilitation can effectively alleviate symptom burden and promote resilience of lung cancer patients undergoing chemotherapy.