Objective　 To construct a detailed mapping of the Chromosome 1pter-p 36.11 deleted region (63.4 cM) in nasopharyngeal carcinoma (NPC) by polymerase c hain reaction-loss of hetrozygosity (PCR-LOH) analysis for the further researc h of NPC-related gene(s). Methods　 Biopsies from 47 cases of NPC patients were studied. DNA extracted from separated cancer cells and their corresponding non- c ancer lymphocytes were amplified with PCR,followed by the analysis of LOH and mi crosatellite instability (MI) for 20 loci spanning Chromosome 1pter-p36.11 regi on with an average interval of 3.0 cM. Results　 82.2% of NPC cases (37/47) showe d at least one loci of LOH. The highest frequency of LOH was found at loci D1S23 4 on 1p36.13 (50.0%), with the LOH at loci D1S2644 on 1p36.22 slightly less (37. 5%). The occurrence of LOH at D1S234 showed no significant difference for the ca ses at early stage and at advanced stage ［60% (9/15) vs 50.0% (8/16) respective ly, P>0.05］. High frequencies of MI were detected at D1S243 on 1p36.33 (37 .5%) and D1S199 on 1p36.21 (30.2%). Conclusions　 There are two common deletion regio ns: one localized at 1p36.13 (D1S234, 2.0 cM) and the other at 1p36.22 (D1S436- D 1S2644, 6.3 cM), with a MI loci at D1S199 between them. This suggests that one or more putative tumor suppressor gene(s) related to the early stage of NPC tumo rigenesis may be encompassed in this zone.

OBJECTIVETo investigate gene expression profile in nasopharyngeaL carcinoma (NPC) cell lines with different metastatic potentialities, in order to identify new candidate genes related to the development, progress and metastasis of NPC.

METHODSThe mRNA expressions of high metastatic NPC cell line 5-8F, tumorigenic but nonmetastatic NPC cell line 6-10B and non-tumorigenic NPC cell line 13-9B (3 sublines of SUNE-1) were investigated by cDNA microarray containing 14 000 cDNA clones. The alterations in gene expression levels were confirmed by reverse-transcription PCR.

RESULTSThere were 82 differentially expressed genes comparing 5-8F and 13-9B; 38 differentially expressed genes comparing 6-10B and 13-9B; 54 comparing 5-8F and 6-10B. There were 12 common differentially expressed genes comparing 6-10B, 5-8F and 13-9B; 14 common differentially expressed genes comparing 5-8F and 13-9B, 6-10B. The expressions of the above genes were involved in metabolism, transcription, differentiation, apoptosis and signal transduction.

CONCLUSIONThe gene expression profile in nasopharyngeal carcinoma cell lines is an important index in the search of new candidate genes related to NPC.

Cell Line ; DNA, Complementary ; analysis ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Neoplasm Metastasis ; genetics ; Oligonucleotide Array Sequence Analysis ; Tumor Cells, Cultured

Objective To study the nursing measures of puncture point bleeding of lung cancer patients with PICC.Methods Clinical data of 56 lung cancer patients with PICC in our hospital was retrospective analyzed,causes of bleeding of puncture point was summarized and corresponding nursing countermeasures were implemented.Results Out of 56 patients with PICC,50 patients were successfully catheterized,there were 11 patients with puncture point bleeding after catheter,but bleeding was stopped and there was no re-catheterization after nursing.Scores of nursing service attitude were (91.25 ± 1.12) points,scores of nursing operation ability was (89.54 ± 2.45) points,nurse-patient communication score was (90.14 ± 1.41) points,health education score was (90.31 ± 2.56) points.And patients were generally satisfied with nursing.Conclusion Nursing staff should remove puncture point bleeding,exudates and blood for patients,replace sterile dressing,and repeat pressure and hemostasis.Situation of the puncture site are viewed again after 24 hours of properly fixing,and catheter is not affected after timely treatment.

Objective To study the nursing measures of puncture point bleeding of lung cancer patients with PICC.Methods Clinical data of 56 lung cancer patients with PICC in our hospital was retrospective analyzed,causes of bleeding of puncture point was summarized and corresponding nursing countermeasures were implemented.Results Out of 56 patients with PICC,50 patients were successfully catheterized,there were 11 patients with puncture point bleeding after catheter,but bleeding was stopped and there was no re-catheterization after nursing.Scores of nursing service attitude were (91.25 ± 1.12) points,scores of nursing operation ability was (89.54 ± 2.45) points,nurse-patient communication score was (90.14 ± 1.41) points,health education score was (90.31 ± 2.56) points.And patients were generally satisfied with nursing.Conclusion Nursing staff should remove puncture point bleeding,exudates and blood for patients,replace sterile dressing,and repeat pressure and hemostasis.Situation of the puncture site are viewed again after 24 hours of properly fixing,and catheter is not affected after timely treatment.

Objective:To explore the effects of peer support-based rehabilitation on symptom burden and resilience of lung cancer patients undergoing chemotherapy, so as to provide reference for relieving the symptoms of patients during chemotherapy.Methods:A total of 90 lung cancer patients who undergoing chemotherapy in Nanjing First Hospital, Nanjing Medical University were assigned to the experimental group and the control group according to the enrolled time, with 45 cases in each group. The patients from January to November 2018 in the control group recieved routine chemotherapy care and rehabilitation, the patients from January to November 2019 in the experimental group implemented peer support-based rehabilitation based on the routine chemotherapy care. The symptom burden and resilience on admission and discharged from hospital were assessed by Chinese version of the MD Anderson Symptom Inventory (MDASI-C) and Connor-Davidson Resilience Scale (CD-RISC), respectively.Results:There was no significant difference in the scores of MDASI-C, CD-RISC on admission between the two groups( P>0.05). At discharge, the symptom distress scores and life influence scores in MDASI-C were (6.19 ± 1.72), (5.72 ± 1.45) points in the experimental group, significantly lower than those in the control group (7.23 ± 2.02), (6.75 ± 1.67) points; the tenacity, power, optimism dementions socres and total scores of CD-RISC were (28.12 ± 4.33), (20.88 ± 5.64), (10.69 ± 3.00), (59.69 ± 8.25) points in the experimental group, significantly higher than those in the control group (24.57 ± 4.14), (17.83 ± 4.09), (9.39 ± 2.58), (51.82 ± 5.84) points. The differences were statistically significant ( t values were 2.18-5.13, all P<0.05). Conclusions:Peer support-based rehabilitation can effectively alleviate symptom burden and promote resilience of lung cancer patients undergoing chemotherapy.

Objective:To explore the effect of silent therapy combined with character strengths-based intervention on the sleep quality and psychological resilience of lung cancer patients undergoing chemotherapy.Methods:From January 2018 to November 2020, the convenient sampling method was used to select 88 lung cancer patients who received chemotherapy in the Nanjing First Hospital of Nanjing Medical University. According to the odd and even number of wards, patients were divided into control group and study group, each with 44 cases. The control group was given routine nursing, and the patients in the study group were treated with silent therapy combined with character strengths-based intervention on the basis of the control group. The differences in sleep quality and psychological resilience between the two groups of patients were observed before and after the intervention for 4 weeks.Results:Before the intervention, there was no statistically significant difference in the scores of sleep quality factors and psychological resilience between the two groups ( P>0.05) . After the intervention, the study group's scores of sleep latency, sleep disturbance, sleep efficiency, drug application, sleep quality, daily function, and sleep duration were all lower than those of the control group, and the psychological resilience score was higher than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The silent therapy combined with character strengths-based intervention applied to the nursing of patients undergoing chemotherapy for lung cancer can effectively improve the sleep quality of patients, increase their psychological resilience, which is worthy of clinical application.