Objective To analyze the influence of diagnosis and surgical treatment of spontaneous rupture and hemorrhage in patients with primary hepatic carcinoma(PHC) on prognosis.Methods 47 consecutive patients with spontaneous rupture and hemorrhage of HCC who had been admitted to our hospital from January 1998 to December 2004 were reviewed retrospectively and followed for 2 years.Results Among the 47 patients,41(87.23%) had liver cirrhosis and 44(93.6%) had been treated by operation. In those cases hepatectomy had been performed in 17 patients and 5 survived for 2 years.The death reasons in ruptured PHC are mainly hepatic function failure, renal function failure and gastrointestinal bleeding.Conclusion The prolonged survival could be achieved through accurately and timely diagnosis and treatment and the resection of the tumor is still a main measure for reducing the mortality in patients with ruptured PHC.

Objective Study the effect of induced differentiation of abscisic acid (ABA) on human HCC cell line SMMC-7221.Methods Cultured SMMC-7221 cells were treated separately with RPMI-1640 culture medium, HMBA and ABA with different concentrations. Firstly, the appropriate concentration of ABA which inhibits SMMC-7221 cell proliferation was selected with the modified MTT method. Then electron microscopy was performed to observe the changes of microstructure. The cell cycle was analyzed by flow cytometry. Results Apart from 4?10 -4mmol/L concentration of ABA, the others could inhibit the cell proliferation. The inhibition rate increased with the time prolonging and the concentration increasing. The effect was most obvious with 4?10 -3mmol/L ABA. At this concentration the cells were arrested in G0/ G1 phase (P

Objective: To investigate the effects of 1, 25-dihydroxy vitamin D₃[1, 25(OH)₂D₃] on food allergy(FA) in mice and its mechanism. Methods: A total of 40 BALB/c mice were randomly divided into 5 groups, 8 in each group, including control group (group C) and FA model group (FA group), according to the dose of 1, 25(OH)₂D₃ intervention, the mice of the FA group were divided into FA₀ group (0), FA_l group [10 μg/(kg·d)], FA_m group [50 μg/(kg·d)] and FA_h group[100 μg/(kg·d)]. Egg albumin was used to establish a food allergy model, with different doses of 1, 25(OH)₂D₃ for gastric intervention, and the control group was replaced by 9 g/L saline.The serum levels of ovalbumin-immunoglobulin E(OVA-IgE), interleukin(IL)-9 and IL-17 of mice were measured by using enzyme linked immunosorbent assay after the last excitation, and HE staining and histopathological examination were carried out in the small intestine of mice. Results: Compared with group C, FA₀ group and FA_h group small intestinal mucosa in mice had different degrees of damage, partial peeling off, structure disorder, villi epithelial cell focal falls peeling off, necrosis, lamina propria edema, congestion, a large number of inflammatory cells infiltration, low but the FA_l group and FA_m group had light mucosa damage, intestinal epithelial basically intact, with integrity, no congestion, edema, and inflammatory cells infiltration to a lesser degree.The mean concentrations of serum IgE, IL-9 and IL-17 in different groups were statistically significant (F=40.770, 9.530, 5.624, all P<0.05). Compared with the FA₀ group [(41.87±3.19) ng/L], the OVA-IgE of the FA_l group [(22.71±4.77) ng/L] and the FA_m group [(16.34±2.81) ng/L] were significantly reduced (t=5.533, 11.835, all P<0.01), but there was no significant difference between the FA_h group [(36.29±6.52) ng/L] (t=1.673, P>0.05). Compared with the FA₀ group [(161.77±50.44) ng/L], the IL-9 levels of the FA_l group [(94.29±18.79) ng/L] and the FA_m group[(84.45±30.88) ng/L] were significantly lower (t=3.267, 3.366, all P<0.01), while that of the FA_h group [(36.29±6.52) ng/L] was not significantly lower (t=0.777, P>0.05). Compared with FA₀ group [(81.55±29.37) ng/L], IL-17 levels of FA_h group [(133.58±47.05) ng/L] was significantly increased (t=2.653, P<0.05), while IL-17 level of FA_l group [(79.41±25.15) ng/L] and FA_m group [(58.81±26.00) ng/L] were lower than that of FA₀ group [(81.55±29.37) ng/L], but the difference was not statistically significant (t=0.154, 1.640, all P>0.05). Conclusions Low, medium dose of 1, 25(OH)₂D₃ supplements can inhibit mice food allergies, but high doses of 1, 25(OH)₂D₃ improve performance in mice food allergies, and 1, 25(OH)₂D₃′s influence on the secretion of IL-9 is one that influences mechanism of food allergy.