Objective : To explore the effects of isogenous micro-grafting(MG) technology and the combined use of botulinum toxin type A(BTX-A) on hair regeneration in mice． Methods : Forty-five C57BL /6 mice were randomly divided into five groups : normal saline group (NS group) ，MG group (MB-0 group) ，MG + low-dose BTX-A group (MB-2 group) ，MG + medium dose BTX-A group ( MB-10 group ) ，MG + high-dose BTX-A group ( MB-50 group) ．The growth of hair on the backs of the mice was observed after the administration of the drugs，and the hair follicles were evaluated by hematoxylineosin ( HE) staining on the 7th ，14th，and 21st days of the experiment， and the expression of vascular endothelial growth factor ( VEGF) and β-catenin was evaluated by immunohisto- chemical staining on day 21 . Results : After treatment with micrograft and botulinum toxin type A，the skin darke- ning time was shortened (P＜0. 05) ，and the coverage rate of new hair increased on the 14 th day (P＜0. 05) . Compared with the NS group，the number of hair follicles increased (P＜0. 05) and the expression of VEGF and β-catenin increased in each other group． Conclusion Isogenous micro-grafting technology combined with botulinum toxin type A has a promotional effect on hair regeneration in mice，and the mechanism may be related to the promotion of vascular growth and activation of β-catenin signaling.