Objective To establish a method for ex vivo cord blood stem cell expansion,increase the number of the stem/progenitor,especially megakaryocyte progenitors so as to decrease the thrombocytopenia period after CBT.Methods Mononuclear cells (MNC) from 20 cord blood samples were cultured in presence of different cytokines:group 1:IL 3,IL 6,group 2:IL 3,IL 6,TPO,group 3:IL 3,IL 6,TPO and cord blood plasma.The final concentration of the cytokines was 50ng/ml.The control group had all medium except the cytokines and cord blood plasma.The cells were cultured for 21 days.At every 7 days,tests were done for nucleate cells number,CFU GM,CFU Mk,and CFU GEMM.Results At different times and in different groups,there were different changes.For NC,maximal increase were obtained in group 3 on day 21,which was 239.65?153.48.For CFU GM,the biggest expansion occurred on day 21 in group 3,the number being 184.17?140.40.For CFU Mk,the biggest expansion was obtained on day 14 in group 3,with the number of 135.34?105.20. The immature progenitor CFU GEMM achieved the max increase in group 3 on day 14,the number was 49.39?36.68. Conclusion The expansion of CFU Mk progenitor cells can be obtained in the presence of IL 3,IL 6,TPO.This combination also enhances the expansion of CFU GM and CFU GEMM.Addition of cord blood plasma can promote the expansion of mature and immature progenitor cells.

OBJECTIVE:To optimize the extraction conditions of volatile oil from Chrysanthemum indicum and the processing techniques of its ?-yclodextrin(?-CD) inclusion compound.METHODS:The extraction conditions of volatile oil from Chrysanthemum indicum was optimized taking soaking time,ultrasonic treatment time,water amount as influencing factors and with the yield of the volatile oil as index.The processing techniques of the ?-yclodextrin (?-CD) inclusion compound was optimized taking the ratio of ?-CD to volatile oil,the time and temperature of the inclusion process as indexes and with the yield of the inclusion compound and the inclusion rate of the volatile oil as indexes. RESULTS:The optimal process for oil extraction was as follows:the soaking time=1.5 h,the ultrasonic treatment time= 40 min,heating time=6 h,water amount=600 mL.The optimal processing conditions for(?-CD) inclusion compound of Chrysanthemum indicum were as follows: the ratio of ?-CD to volatile oil=8∶1,the inclusion time of the compound was 2 hours at 40 ℃.CONCLUSION:High extraction yield of vlatile oil and stable inclusion compound were obtained from the above method.

Objective: Design a test phantom with a full function, simple structure and high accuracy which is used for MRI’s Quality Control. Methods: Through a intensive study in imaging principle of MRI and parameters which effect the image quality ,first come up with the phantom’s performance data, and then determine the technical solutions including the choosing material, processing technology, the machining precision, at last develop a trial set of MRI test phantom using the method of numerical control machining. Make a comparative experiment between this phantom and the similar imported one to analyze the consistency of the results by the two phantoms. Results: The result of all the technical parameters of this phantom is consistent with the result of the imported one. There are some extra test components which the imported phantom doesn’t contain. Some performance data like the low contrast resolution are superior to the imported phantom. Conclusion:This MRI test phantom can satisfy the requirement of the daily quality control work in hospital.

Objective To study the causes of conversion to open surgery in patients receiving gynecological laparoscopic operations. Methods From January 2002 to December 2007,totally 2630 patients received gynecological laparoscopy in our hospital; 45 of them were converted to open surgery. The data of the 45 cases were analyzed. Results The causes of conversion to open surgery included uterus myoma located at specific locations (25 cases,55.6%),severe pelvic-abdominal adhesion (10 cases,22.2%),uterine horn pregnancy (3 cases,6.7%),ovarian tumors (3 cases,6.7%),surgical injury (3 cases,6.7%) ,and adenomyoma with requirement for uterus conservation (1 case,2.2%). Conclusions Peculiar locations of uterine myoma and severe pelvic-abdominal adhesion are the main causes of conversion to open surgery during gynecological laparoscopy. Detailed preoperative evaluation can decrease the rate of the conversion rate. Right timing of the conversion may reduce complication rate.

0.05). The laser group with the dose of 3 J?cm-2 showed more increase in cell survival at 24 and 48 h after being seeded as compared with control group(P

Objective To investigate the restricting factors of long-term care of stroke survivors in community.Methods The phenomenological methodology was adopted in the study.Sixteen stroke survivors were interviewed.Data were analyzed by Colaizzi's method.Results Three themes were found,including physical barriers,incorrect attitude towards disease,and defects of community health service.Conclusions On the basis of improving technology and ability,medical workers in community should consider of supporting the patients with information of disease and useful resources in a variety of ways,and improving the patients health literacy.

Purpose:To study the distribution of blood pressure in the cardiovascular system of rat. Method:The experimental animal is Wistar rat (n=lS). The blood pressure in different parts of the cardiovascular system were recorded with a 4-channel polygraph system under closing condition. This method is more exact than that of manometer. Results:Our experiments demonstrated the following distribution of blood pressure in the cardiovascular system of rat. (1) The left ventricular systolic pressure(LVSP ) was 145.00+15.16 mmHg and the aortal systolic pressure(ASP ) was 126.87+11.16 mmHg. The LVSP was higher than ASP. The right ventricular systolic pressure(RVSP ) was 35.04+3.95 mrnHg and the pulmonary arterial systolic pressure(PASP) was 27.0313.46 mmHg. The RVSP was higher than PASP. This distribution of blood pressure was different with human. (2) On Value and range, the blood pressure in vascular system of rat (including A SP、A DP、MA P、PA SP、PA DP、MPA P、CVP、 WP)were clese to human. Conclusion: (1) The LVSPwas higher than ASP and the RVSP was higher than PASP. This distribution of blood pressure of rat was different with human. (2) The LVSP and RVSP of rat were higher when compared with human and the blood pressure distribution of other parts in the vascular system of rat was close to human

Objective To study the impact of lung volume reduction surgery on inflammatory factors, pulmonary function and quality of life in patients with severe chronic obstructive pulmonary emphysema. Methods 57 cases patients with severe chronic obstructive pulmonary emphysema received lung volume reduction surgery from May 2009 to December 2013 were divided into observation group 32 cases and control group 25 cases, the control group were given open chest surgery, the observation group received video-assisted thoracoscopic surgery. Then compare the operation indicator, serum inflammatory factor content, pulmonary function and life quality score between the two groups. Results Operation indicators: Observation group: Intraoperative blood loss, thoracic drainage, hospital stay were significantly lower than that in control group (P< 0.05); Inflammatory factor: 3 d after surgery, observation group serum TNF-α, IL- 6, IL- 1 content were significantly lower than that in control group (P<0.05);Pulmonary function: 12 months after surgery, there were no statistical difference of FEV1, TLC, RV between two groups (P>0.05); SGRQ score: 12 weeks after the surgery, observation group respiratory symptoms, activity ability, disease im﹣pact, SGRQ total score were significantly lower than the control group (P< 0.05). Conclusion Video assisted tho﹣racic surgery helps to reduce surgical trauma, and alleviate inflammatory reaction, then improve the quality of life.

Objective To provide information and assistance for research of bile duct injury in OLT through endoscopic observation and treatment of biliary complications after liver transplantation.Methods After OLT, all the cases in normal group, bile duct injury group and hepatic artery injury group were observed, diagnosed and recorded respectively. Meanwhile, the biopsy was performed through the endoscopy for pathological examination. For those cases without T tube, the biopsy was conducted by choledochoscopy in combination with duodenoscopy. Results The exterior and interior bile duct anatomy of the 9 cases in the normal group was normal. They had no bile duct stenosis and scar, their bile duct mucous membrane looked good and the anastomosis of the donor-receptor bile duct healed well. Restored mucous tissue coating with intact epithelium was found by pathological examination. Fibrous tissue and small vascular proliferation happened under epithelium scattered with plasmocyte and lymphocyte. Various kinds of bile duct stones-simple, multiple and casting mould type, were found in 12 cases with bile duct injury. Bile duet mucous membrane injured in different degrees was repaired after stone removed and obstruction relieved by endoscope. Bile duct tree becoming normal was seen by pacification examination. Three cases in the hepatic injury group had bile duct ischemic necrosis, losing of normal structure without bile duct wall and mucous membrane. Conclusion Bile ducts are injured in different degrees in OLT. The choledochofibroscopy is of the first choice for diagnosis and treatment of complications after OLT.