Objective To study the result of Alexandrite laser hair removal after subcutaneous in-jection of?-MSH in anagen-induced C57BL6mice.Methods Hair shafts were depilated by wax/resin mix-ture to induce hair follicles from telogen to anagen in60C57BL6mice.The mice were randomly divided in-to groups A,B,C and D.Groups A and B were injected with0.5mg/kg and0.25mg/kg of?-MSH,respec-tively,on the back skin subcutaneously once a day.Group C was injected with the same dose of normal saline.Group D was treated as blank control.Groups A,B and C were exposed to Alexandrite laser on ana-gen(substageⅣ).Biopsies were taken before treatment and0.5h,2and28days after treatment.Speci-mens were stained with Masson-Fontana method before treatment,and with haematoxylin and eosin after treatment.The cutaneous response was observed after laser hair removal.Hair regrowth was assessed28days after treatment.Results The mean gradation value of folliclar melanin was increased in the test groups than that in control group before laser hair removal.Extent of folliclar damage and cutaneous adverse reaction af-ter laser treatment was more severe in test groups than those in control group.Hair regrowth was less obvious in test groups than that in control group,while local hyperpigmentation was increased in test groups than that in control group28days after treatment.No scarring was observed in3groups.Conclusion Subentaneous injection of?-MSH could increase melanin of the hair,decrease hair regrowth,and enhance local pigmenta-tion after laser hair removal in anagen-induced C57BL6mice.

Objective To investigate the concentration of 17β-estradiol in bone marrow stem cells (BMSCs) and different tissues of rats, and study the function of estradiol produced in extragonadal sites prelimi-narily. Methods (1) The concentration of 17β-estradiol in lysate of BMSCs and in supernatant cell culture media were detected by Elisa method after rats BMSCs were serum-free cultured in 0 h , 24 h and 48 h respective ly . (2) The tissues of organs were grinded and broken under ultrasonic wave , then washed and weighed. The con-centration of 17β-estradiol in different tissues was detected by Elisa method. Results Compared with the concen-tration of 17β-estradiol at 24 h, the concentration of 17β-estradiol at 48h significantly increased (P < 0.05). The concentration of 17β-estradiol among different tissues were higher, but the difference is not significant (P > 0.05). Conclusions (1) 17β-estradiol can be secreted by BMSCs , and the concentration is proportional to the time to some degree. But whether the extragonadal estrogen can function locally is still unclear. (2) The concentration of 17β-estradiol is non- gonad dependent but whether it is secreted locally remains to be elucidated. The non- gonad estrogen could be the estrogen source of bone metabolism in order to sustain bone health after menopause.

BACKGROUND:It is suggested that glucose metabolism of frontal lobes,cingulate gyri and caudate nuclei in cerebral functional area of patients with depression is low,and the blood flow in cerebellum decreases.OBJECTIVE:To observe the effect of scalp acupuncture on cerebral glucose metabolism in depression by positron emission tomography.DESIGN:Randomly controlled and verifying experiment.SETTING:Department of Acupuncture and Aoxibustion in Nanfang Hospital and Center of Positron Emission Tomography in a Guangdong People's hospital.PARTICIPANTS:Twelve patients of primary depression,from Nanfang Hospital,Zhujiang Hospital and Center of Positron Emission Tomography in Guangdong People's Hospital from October 2002 to October 2003,were selected randomly with the diagnostic criteria of [F32] in the Third Edition of the Chinese Classification and Diagnostic Criteria of Mental Disease.Among them,3 were male and 9 were female.Their ages were from 36 to 66 years,with the average of 48.METHODS:Giving scalp acupuncture in middle line of vertex(MS5),middle line of forehead(MS1) and bilateral lateral line 1 of forehead(MS2).All the patients received positron emission tomography examination before and after acupuncture treatment for 6 weeks.Then cerebral Regions of Interest,bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella,were chosen and their average glucose metabolisms were counted by half-quantifying analysis.MAIN OUTCOME MEASURES:Glucose metabolism of bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella.RESULTS:According to intension,all 12 patients with primary depression sults of right frontal lobes(2.91 ±0.34),left frontal lobes(2.96±0.26),right cingulate gyri(2.68±0.20),right caudate nuclei (2.61±0.39) and left cerebellum(2.55±0.18)were obviously higher than those of right frontal lobes(2.53±0.31),left frontal lobes(2.53±0.36),right cingulate gyri(2.34±0.31),right caudate nuclei(2.40±0.33) and left cerebellum(2.35±0.25)before treatment(P＜ 0.05-0.01).However, the results of left cingulate gyri (2.69 ±0.38),left caudate nuclei(2.66±0.01) and right cerebellum(2.50±0.27) were similar to those of left cingulate gyri(2.43±0.38),left caudate nuclei(2.45±0.21) and right cerebellum(2.30±0.34) before treatment(P ＞ 0.05).CONCLUSION:Scalp acupuncture on middle line of vertex,middle line of forehead and bilateral lateral line 1 of forehead could improve the glucose metabolism in depressive patients of certain cerebral regions,and it might be a mechanism related to improvement of frontal lobes,caudate nuclei,cingulate gyri and cerebellum.

Objective To study the impact of lung volume reduction surgery on inflammatory factors, pulmonary function and quality of life in patients with severe chronic obstructive pulmonary emphysema. Methods 57 cases patients with severe chronic obstructive pulmonary emphysema received lung volume reduction surgery from May 2009 to December 2013 were divided into observation group 32 cases and control group 25 cases, the control group were given open chest surgery, the observation group received video-assisted thoracoscopic surgery. Then compare the operation indicator, serum inflammatory factor content, pulmonary function and life quality score between the two groups. Results Operation indicators: Observation group: Intraoperative blood loss, thoracic drainage, hospital stay were significantly lower than that in control group (P< 0.05); Inflammatory factor: 3 d after surgery, observation group serum TNF-α, IL- 6, IL- 1 content were significantly lower than that in control group (P<0.05);Pulmonary function: 12 months after surgery, there were no statistical difference of FEV1, TLC, RV between two groups (P>0.05); SGRQ score: 12 weeks after the surgery, observation group respiratory symptoms, activity ability, disease im﹣pact, SGRQ total score were significantly lower than the control group (P< 0.05). Conclusion Video assisted tho﹣racic surgery helps to reduce surgical trauma, and alleviate inflammatory reaction, then improve the quality of life.

Objective To study the effect of intranasal nerve growth factor (NGF) on the expression of amyloid-β,peptide (Aβ) in the central nervous system in rats with traumatic brain injury (TBI).Methods Eighty rats were randomly divided into sham(n =26),control(n =27) and treatment group (n =27 ).They were subjected to the modified Feeney' s weight-drop model.The treatment group was treated with NGF administered by nasal route,and the control group was given phosphate-buffered saline (PBS).Beam walking and Morris water maze test were performed in the three groups.The concentration of Aβ40 and Aβ42 in the injured ipsilateral hippocampus was elevated by ELISA measurement.Immunohistochemistry was used to detect the amyloid precursor protein (APP) positive cells near the region of injury in the hippocampus in rats after TBI.Results NGF group traversed the beam significantly quicker (s) than control group ( 19.00 + 6.99 vs 27.33 ± 7.39 respectively,F2,15 =12.87,P =0.028 ).Morris water maze performance revealed that mean time of latency in the NGF group was significant shorter than vehicle group,and significant memory retention in NGF group as evidenced by a greater percentage of the 60 s allotted time spent in the target quadrant (45.82％ ± 11.15％ vs 33.99％ ± 3.46％,F2,15 =6.814,P=0.037),as well as the number crossing of the former site of the removed platform in NGF group was significant more than control group (8.60 ±2.73 vs 3.60 ±2.06,F2,15 =5.346,P =0.04).The Aβ42 level in control group was increased significantly higher than NGF group as indicated by ELISA measurements.While the Aβ40 level did not have similar shown.Immunohistochemical staining showed that APP level had significant differences among three groups ( F2,15 =8.672,P =0.003).The APP level in NGF group did not alter with control group.Conclusion Intranasal administration of NGF can regulate Aβ42 overproduction,improve the motor and cognitive function after brain injury in rats.

Objective To investigate the relationship between the serum SRC-3 levels and the bone loss severity in postmenopausal women. Methods Fifty-eight PMW with osteopenia or osteoporosis and nineteen healthy PMW were enrolled in this study from June 2012 to September 2013. BMD at the lumbar spine and femoral neck were observed by DXA Lunar Prodigy Vision. The levels of serum SRC-3 were detected by ELISA. The diagnosis value was evaluated by the ROC curves analysis. Results The levels of serum SRC-3 were significant higher in the normal group than those in the osteopenia or the osteoporosis groups (P<0.001 for both), no statistical significance was found between the osteopenia and the osteoporosis group(P=0.056). The levels of serum SRC-3 were negatively correlated with the BMD diagnosis grading (r=-0.543, P < 0.001). By using the ROC curve analysis, the serum level of SRC-3 for PMW with osteoporosis and osteopenia were found to be 0.297 ng/mL and 0.347 ng/mL, respectively. The levels of serum SRC-3 were positively associated with BMI (r=0.395, P<0.001) and LS-BMD (r=0.503,P<0.001) in the postmenopausal women. Conclusion SRC-3 might be an useful index to reflect the severity of lumbar spine bone loss.

BACKGROUND:Estrogen signaling pathway for interaction between aromatase and estrogen-related receptor may exist in bone marrow mesenchymal stem cel s, which is used for regulating biological activity of bone marrow mesenchymal stem cel s. OBJECTIVE:To observe the expression of aromatase and estrogen-related receptors in adult bone marrow mesenchymal stem cel s during osteogenic differentiation. METHODS:Bone marrow mesenchymal stem cel s were respectively cultured in low-glucose DMEM medium (control group) and osteogenic induction medium (induction group). Cel proliferation and calcium deposition were determined by MTT assay and alizarin red staining, respectively. The expression of aromatase, estrogen receptorα, estrogen receptorβ, and estrogen-related receptorαduring osteogenic differentiation were determined by real-time PCR and western blot analysis. Estradiol levels in supernatants and lysates were detected by ELISA method. RESULTS AND CONCLUSION:In the induction group, the proliferation ability of bone marrow mesenchymal stem cel s was the strongest at 72 hours of culture;while there were a great amount of calcium nodules formed at 21 days of culture. Results from PCR and western blot assay showed that the expression of aromatase and estrogen receptorαwas improved in the induction group, but the expression of estrogen-related receptorαwas inhibited. There was no difference in the expression of estrogen receptorβbetween the two groups. ELISA results indicated that the level of estradiol in the supernatant of induction group was the highest. These findings indicate that aromatase, estrogen receptorα, estrogen receptorβand estrogen-related receptorαare al involved in osteogenesis of bone marrow mesenchymal stem cel s. Moreover, estradiol can be synthesized and secreted in bone marrow mesenchymal stem cel s, and most likely, promote the osteogenic differentiation of bone marrow mesenchymal stem cel s by related receptor pathway.

Objective To investigate the magnetic resonance imaging(MRI) in the diagnosis of congenital anorectal malformation.Methods Fourteen patients with congenital anorectal malformation received pelvic and sacrococcygeal MRI scan with 5 normal controls.The morphological changes of puborectalis and anal sphincter,and the abnormalities of anus,rectum,sacral vertebra and genitourinary system were observed.Results The best developed puborectalis and anal sphincter were showed in 13 cases,the better developed in 3 cases,the least developed in 3 cases,respectively.There were 7 cases with abnormalities of sacral vertebra and 5 cases with abnormalities of genitourinary system.Conclusion MRI examination plays an important role in the diagnosis of congenital anorectal malformation.The morphological changes of puborectalis and anal sphincter,and the abnormalities of sacral vertebra and genitourinary system can be determined by the MRI examination, which is important in clinical therapy planning and accessing the curative effect.

Postmenopausal osteoporosis(PMOP)is a primary metabolic disorder of bone in postmenopausal women which is characterized by osteopenia, destruction of bone microstructure, increased bone fragility and fracture.PMOP is the major cause of joint pain and disability in middle-aged and elderly women.The research shows that early biomarkers can provide clinical evidence for early diagnosis of PMOP, assess fracture risk and evaluate treatment effect.In this paper, the latest advances in the application of PMOP biomarkers are described from the aspects of PMOP hormone correlation index and bone metabolism.

Objective:To evaluate intestinal stenting in patients of left colon cancer complicated with acute intestinal obstruction and analyze its short-term and long-term efficacy.Methods:Sixty patients who suffered from left colon cancer complicated with acute intestinal obstruction at the First Department of General surgery, Chengdu Second People's Hospital from Sep 2016 to Sep 2019 were retrospectively enrolled. According to treatment methods, the patients were divided into the stent implantation staged surgery group (24 patients ) and the control group receiving emergency surgery (36 patients).Results:There was no significant difference in perioperative course between the two groups. There was no significant difference in three year overall survival rate or recurrence and metastasis rate between the two groups. Preoperative bowel preparation rate in stent implantation group was obviously higher than the control group (100% vs. 44%, P=0.000). The laparoscopic surgery rate of the stent implantation group was higher than the control group (21% vs. 0, χ2=9.857， P=0.002). The enterostomy rate of the stent implantation group was lower than the control group (0 vs. 19%, χ2=10.237， P=0.005). There was a higher rate of one-stage excision and anastomosis in the stent implantation group than in the control group (100% vs. 81%, χ2=7.760， P=0.005).There was a higher lymph nodes deissection rate in the stent implantation group than in the control group, [11(7-14) vs. 6(3-10), Z=-2.672， P=0.006]. Conclusions:For patients who suffered from left colon cancer complicated with acute intestinal obstruction, the intestinal stent can improve the bowel preparation to a greater extent, which can increase the proportion of one-stage anastomosis and reduce enterostomy rate.