1.Extraction of Volatile Oil from Chrysanthemum indicum and Preparation Techniques of Its ?-cyclodextrin Inclusion Compound
China Pharmacy 2007;0(36):-
OBJECTIVE:To optimize the extraction conditions of volatile oil from Chrysanthemum indicum and the processing techniques of its ?-yclodextrin(?-CD) inclusion compound.METHODS:The extraction conditions of volatile oil from Chrysanthemum indicum was optimized taking soaking time,ultrasonic treatment time,water amount as influencing factors and with the yield of the volatile oil as index.The processing techniques of the ?-yclodextrin (?-CD) inclusion compound was optimized taking the ratio of ?-CD to volatile oil,the time and temperature of the inclusion process as indexes and with the yield of the inclusion compound and the inclusion rate of the volatile oil as indexes. RESULTS:The optimal process for oil extraction was as follows:the soaking time=1.5 h,the ultrasonic treatment time= 40 min,heating time=6 h,water amount=600 mL.The optimal processing conditions for(?-CD) inclusion compound of Chrysanthemum indicum were as follows: the ratio of ?-CD to volatile oil=8∶1,the inclusion time of the compound was 2 hours at 40 ℃.CONCLUSION:High extraction yield of vlatile oil and stable inclusion compound were obtained from the above method.
2.Effect of electro-scalp acupuncture on metabolism of glucose in brain functional region of patients with depression
Yong HUANG ; Anwu TANG ; Qiushi LI ; Dongjiang LI ; Jing CHEN
Chinese Journal of Tissue Engineering Research 2005;9(20):252-253
BACKGROUND:It is suggested that glucose metabolism of frontal lobes,cingulate gyri and caudate nuclei in cerebral functional area of patients with depression is low,and the blood flow in cerebellum decreases.OBJECTIVE:To observe the effect of scalp acupuncture on cerebral glucose metabolism in depression by positron emission tomography.DESIGN:Randomly controlled and verifying experiment.SETTING:Department of Acupuncture and Aoxibustion in Nanfang Hospital and Center of Positron Emission Tomography in a Guangdong People's hospital.PARTICIPANTS:Twelve patients of primary depression,from Nanfang Hospital,Zhujiang Hospital and Center of Positron Emission Tomography in Guangdong People's Hospital from October 2002 to October 2003,were selected randomly with the diagnostic criteria of [F32] in the Third Edition of the Chinese Classification and Diagnostic Criteria of Mental Disease.Among them,3 were male and 9 were female.Their ages were from 36 to 66 years,with the average of 48.METHODS:Giving scalp acupuncture in middle line of vertex(MS5),middle line of forehead(MS1) and bilateral lateral line 1 of forehead(MS2).All the patients received positron emission tomography examination before and after acupuncture treatment for 6 weeks.Then cerebral Regions of Interest,bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella,were chosen and their average glucose metabolisms were counted by half-quantifying analysis.MAIN OUTCOME MEASURES:Glucose metabolism of bilateral frontal lobes,bilateral caudate nuclei,bilateral cingulate gyri and bilateral cerebella.RESULTS:According to intension,all 12 patients with primary depression sults of right frontal lobes(2.91 ±0.34),left frontal lobes(2.96±0.26),right cingulate gyri(2.68±0.20),right caudate nuclei (2.61±0.39) and left cerebellum(2.55±0.18)were obviously higher than those of right frontal lobes(2.53±0.31),left frontal lobes(2.53±0.36),right cingulate gyri(2.34±0.31),right caudate nuclei(2.40±0.33) and left cerebellum(2.35±0.25)before treatment(P< 0.05-0.01).However, the results of left cingulate gyri (2.69 ±0.38),left caudate nuclei(2.66±0.01) and right cerebellum(2.50±0.27) were similar to those of left cingulate gyri(2.43±0.38),left caudate nuclei(2.45±0.21) and right cerebellum(2.30±0.34) before treatment(P > 0.05).CONCLUSION:Scalp acupuncture on middle line of vertex,middle line of forehead and bilateral lateral line 1 of forehead could improve the glucose metabolism in depressive patients of certain cerebral regions,and it might be a mechanism related to improvement of frontal lobes,caudate nuclei,cingulate gyri and cerebellum.
3.Effect of berberine combined with levofloxacin on myocardial enzymes, isozymes and serum inflammatory factors in children with diarrhea
Hao CHEN ; Qiushi LIU ; Qingsheng LE ; Xing WANG
Chinese Journal of Biochemical Pharmaceutics 2015;(9):97-99
Objective To analyse effect of berberine combined with levofloxacin on myocardial enzymes, isozymes and serum inflammatory factors in children with diarrhea.Methods 58 patients who were diagnosed with pediatric infectious diarrhea in our hospital were collected.All patients were randomly divided into experimental group and control group, 29 cases in each group.two groups of patients were given routine treatment, including infusion, maintaining water and electrolyte balance, supplemental calories etc.On the basis of routine treatment, control group was intravenous dripped with levofloxacin injection 200mL,one times per day,and experimental group was treated with berberine hydrochloride tables 0.2 g orally on the basis of control group.The treatment period was one week of two group.After one week of treatment, the serum levels of myocardial enzymes, isozymes, inflammatory factors and clinical curative effect were detected in all patients.Results Compared with control group post-treatment,the serum AST,ALT and LDH levels were lower than that of experimental group(P<0.05);the serum CK and CK-MB levels were lower than that of experimental group(P<0.05);the serum TNF-α,IL-6 and IL-10 levels were lower than that of experimental group(P<0.05);the total effective rate was higher in experimental group(P<0.05).Conclusion The berberine combined with levofloxacin can significantly reduce the serum myocardial enzymes, isozymes and serum inflammatory factors in children with diarrhea, improve clinical curative effect.
4.Angiogenic factors in osteonecrosis of the femoral head
Guoju HONG ; Wei HE ; Qiushi WEI ; Leilei CHEN
Chinese Journal of Tissue Engineering Research 2016;20(15):2197-2205
BACKGROUND: A variety of angiogenic factors are involved in bone healing after osteonecrosis of the femoral head.
OBJECTIVE:To explore the role and mechanism of angiogenic factors in osteonecrosis of the femoral head.
METHODS: A computed-based online search of PubMed, Google and SpringerLink databases was performed using the key words of “angiogenic factors, osteonecrosis of the femoral head, vascular endothelial growth factor, angiopoietin-1, fibroblast growth factor-2, hypoxia inducible factor-1, calcitonin gene related peptide and hypoxia inducible factor-1α” for literatures published from December 1980 to May 2015.
RESULTS AND CONCLUSION:Finaly, 68 articles were included. Bone angiogenesis which is dependent on special signaling factors in the microenvironment is closely linked with bone repair. A variety of cytokines, such as vascular endothelial growth factor, angiopoietin-1, fibroblast growth factor-2, platelet-derived growth factor, calcitonin gene-related peptide, and hypoxia inducible factor-1α, have been identifiedto control angiogenesis in different ways and be involved in the repair of necrotic femoral head.
5.Analysis of restricting factors of long-term care of stroke survivors in community
Yaqi ZHANG ; Zhenxiang ZHANG ; Ying CHEN ; Lamei LIU ; Qiushi ZHANG
Chinese Journal of Practical Nursing 2014;30(8):8-10
Objective To investigate the restricting factors of long-term care of stroke survivors in community.Methods The phenomenological methodology was adopted in the study.Sixteen stroke survivors were interviewed.Data were analyzed by Colaizzi's method.Results Three themes were found,including physical barriers,incorrect attitude towards disease,and defects of community health service.Conclusions On the basis of improving technology and ability,medical workers in community should consider of supporting the patients with information of disease and useful resources in a variety of ways,and improving the patients health literacy.
6.Impact of lung volume reduction surgery on inflammatory factors, pulmonary function and quality of life in patients with severe chronic obstructive pulmonary emphysema
Xiaojie ZHANG ; Zhiming CHEN ; Qiushi ZHANG ; Shien HUANG ; Min DENG
China Journal of Endoscopy 2016;22(7):14-17
Objective To study the impact of lung volume reduction surgery on inflammatory factors, pulmonary function and quality of life in patients with severe chronic obstructive pulmonary emphysema. Methods 57 cases patients with severe chronic obstructive pulmonary emphysema received lung volume reduction surgery from May 2009 to December 2013 were divided into observation group 32 cases and control group 25 cases, the control group were given open chest surgery, the observation group received video-assisted thoracoscopic surgery. Then compare the operation indicator, serum inflammatory factor content, pulmonary function and life quality score between the two groups. Results Operation indicators: Observation group: Intraoperative blood loss, thoracic drainage, hospital stay were significantly lower than that in control group (P< 0.05); Inflammatory factor: 3 d after surgery, observation group serum TNF-α, IL- 6, IL- 1 content were significantly lower than that in control group (P<0.05);Pulmonary function: 12 months after surgery, there were no statistical difference of FEV1, TLC, RV between two groups (P>0.05); SGRQ score: 12 weeks after the surgery, observation group respiratory symptoms, activity ability, disease im﹣pact, SGRQ total score were significantly lower than the control group (P< 0.05). Conclusion Video assisted tho﹣racic surgery helps to reduce surgical trauma, and alleviate inflammatory reaction, then improve the quality of life.
7.Characteristics of volunteer blood donors with regular apheresis platelets in Zhoushan City
Heng WANG ; Yanlin CHEN ; Haihong WANG ; Qiushi WANG ; Junping BAO
Journal of Preventive Medicine 2022;34(10):1015-1019
Objective:
To investigate the characteristics of volunteer blood donors with regular apheresis platelets in Zhoushan City, Zhejiang Province, so as to provide insights into recruitment of regular blood donors.
Methods:
Gender, age, occupation, educational level, and volume and times of blood donation were collected from volunteer blood donors with apheresis platelets in Zhoushan Central Blood Station during the period from 2019 to 2021 through the Zhejiang Provincial Blood Management Information System BIS version 3.0, and the willingness of blood donation, routes for acquiring blood donation knowledge and cause of blood donation were collected from volunteer blood donors with regular apheresis platelets using questionnaire surveys. The factors affecting regular apheresis platelets were identified among volunteer blood donors using a multivariable logistic regression model.
Results:
Totally 236 volunteer blood donors participated in regular apheresis platelets in Zhoushan City from 2019 to 2021, accounting for 51.08% of all volunteer blood donors with regular apheresis platelets, and there were 1 488 blood donations, accounting for 71.23% of all volunteer blood donations with apheresis platelets. A total of 2 086.00 U platelets were donated, accounting for 71.82% of all volumes of volunteer blood donations with apheresis platelets. The volunteer blood donors included 160 men (67.80%) and 76 women (32.20%) and had an age of 35 (17) years. There were 63 employees (26.69%) and 111 with a bachelor degree and above (47.03%). Multivariable logistic regression analysis showed a high proportion of regular blood donations among participants at ages of 31 to 60 years (OR:1.767-3.257, 95%CI: 1.010-6.250), university students (OR=2.598, 95%CI: 1.238-5.450) and civil servants (OR=2.733, 95%CI: 1.193-6.263). There were 228 participants with willingness to continue to participate in volunteer blood donation with regular apheresis platelets (96.61%), and 215 participants with a history of participating in volunteer blood donation surveys (91.10%). Internet was the predominant route for acquiring knowledge about apheresis platelet donation (41.10%), and “think that blood donation is a meaningful thing” was the predominant cause of participating in volunteer apheresis platelet donation (33.05%).
Conclusions
There is a high proportion of regular blood donation among civil servants and university students.
8.Analysis of the status of stroke survivors' adherence to functional exercise in the community and countermeasures
Beilei LIN ; Zhenxiang ZHANG ; Ying CHEN ; Yanhong LI ; Qiushi ZHANG ; Suyan CHEN ; Fayang MA
Chinese Journal of Physical Medicine and Rehabilitation 2013;35(9):722-726
Objective To analyse the adherence of community-dwelling stroke survivors to functional exercise regimens and explore the influencing factors.Then to establish measures to improve the situation.Methods A total of 208 stroke survivors from 10 communities were randomly recruited.Four questionnaires-a general information questionnaire,an exercise adherence questionnaire,a self-efficacy perceptions scale and a perceived social support scale-were administered.Results The mean adherence score was 34.71 ± 8.45 and the mean adherence rate was 61.98%.The compliance to physical exercise,seeking advice positively and exercise effectiveness monitoring scores ranged from highest to lowest in order.Self-efficacy perceptions and perceived social support were both positively correlated with the adherence rate.Multiple linear regression showed that the influencing factors included self efficacy perceptions,social support perceptions,self-care ability and age.Conclusions The community stroke survivors' exercise adherence was moderate in general,but it could be improved by increasing their self-efficacy and social support perceptions and their self-care ability.
9.Practice and construction of teaching team of specialized courses for nurses in ICU
Aiqin SONG ; Pengfei LIU ; Huanjun XIA ; Qiushi LIU ; Chen LI ; Ling SHANG ; Bo BAN
Chinese Journal of Medical Education Research 2012;11(9):950-952
As a pilot program of teaching reform,some excellent undergraduates majoring in nursing were selected to take specialized courses in ICU in order to cultivate the intensive care specialized nurses.The teaching team of specialized courses in ICU was established to guarantee the teaching quality.The team was in charge of the ICU specialized curriculum building,textbooks selection and teaching content examination.The high leveled teaching team of specialized courses in ICU was organized by taking teaching and researching section as a unit to improve theoretical teaching level,using clinical department as training base to enhance practice skill and participating in special subject classes and in-service study to promote specialized quality.
10.A kidney-tonifying herbal fufang effects the bone mineral density in senile osteoporosis mice by GH/IGF-1 axis
Hairong SU ; Lulu TANG ; Renfei YUAN ; Xiaoxiang CHEN ; Qiushi WEI ; Weimin DENG
The Journal of Practical Medicine 2017;33(15):2459-2464
Objective To study whether the effects of bone mineral density by a kidney-tonifying herbal fufang treatment in senile osteoporosis mice (P6) is by the mechanism of improving the expression level of GH mRNA and IGF-1 mRNA. Methods The experimental points four groups as following:SAMR1 mice which feed saline lavage,SAMP6 divid as saline lavage group,subcutaneous injection of rhGH group and a kidney-tonifying herbal fufang treatment group. All intervention is one time everyday. After 3 months and 6 months intervention,we measure the BMD and the expression level of the GH mRNA and of IGF-1 mRNA. Results After 3 months intervention,the BMD of R1 group and the Kidney group were higher than the P6 blank group;but there is no difference in BMD between RhGH group and the P6 blank group. The effect of GH mRNA and IGF-1 mRNA expression levels:the R1 group,rhGH and kidney group were higher than the P6 blank group. After six months intervention,the BMD of the rhGH group and kidney group are higher than the P6 blank group. GH mRNA and IGF-1 mRNA expression levels:GH group and kidney group are higher than the P6 blank group. The expression level of GH mRNA and IGF-1 mRNA in four groups has positive correlation. After six months intervention ,we found the positive correlation between the expression level of GH mRNA and IGF-1 mRNA and each part of the whole body BMD. Conclusion A kidney-tonifying herbal fufang can improve the bone mineral density of P6,and its mechanism may be related to improve expression level of GH mRNA and IGF-1 mRNA.