1.Detection of exhaled nitric oxide of healthy in Nanjing.
Gaiyan LENG ; Zeqing LI ; Qiuping WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(17):769-771
OBJECTIVE:
To determine the normal level of nitric oxide through mouth and nose in healthy adult people. From it the normal values were obtained to provide theory support for the prevention and cure of breathe system diseases, such as asthma, allergic rhinitis and so on.
METHOD:
One hundred and eighty two healthy nanjing adults were recruited,including 89 males and 93 females. NIOX system was used to obtain the values of exhaled NO through mouth and nose. The relativity between NO and gender, age, height, body mess index, time, ambient NO were analyzed with multiple linear regression and correlation.
RESULT:
Exhaled NO values were (17 +/- 7) ppb and correlated significantly with height and age. Regression equation: Y (FENO) = -65.784 + 0.102X1 (age) + 0.442X2 (height), P < 0.05. NNO values were (79 +/- 35) ppb.
CONCLUSION
FENO normal values and NNO normal values are easy to obtain and have good compliance and high sensitivity, which correlated with height, age, et al.
Adolescent
;
Adult
;
Aged
;
Asthma
;
diagnosis
;
Breath Tests
;
China
;
Exhalation
;
Female
;
Humans
;
Male
;
Middle Aged
;
Nitric Oxide
;
analysis
;
Reference Values
;
Rhinitis, Allergic, Perennial
;
diagnosis
;
Young Adult
2. Analysis the methylation of caveolin-2 gene in peripheral blood of uygur pigeon feeders
Qiuping LENG ; Wenyi WANG ; Chao WU ; Xiaohong YANG
Chinese Journal of Postgraduates of Medicine 2019;42(12):1057-1060
Objective:
To study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2.
Methods:
Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2.
Results:
CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (
3.Analysis of clinical characteristics of lung adenocarcinoma patients with exon 21 L858R deletion mutation
Qiuping LENG ; Jie JIAO ; Xiaohong YANG
Cancer Research and Clinic 2020;32(4):271-274
Objective:To explore the clinical characteristics of lung adenocarcinoma patients with exon 21 L858R deletion mutation.Methods:The data of 112 patients who were diagnosed with lung adenocarcinoma and positive genetic mutations in the People's Hospital of Xinjiang Uygur Autonomous Region from January 2015 to December 2018 was retrospectively analyzed, and the patients were divided into the exon 21 L858R deletion mutation group (52 cases) and the non-exon 21 L858R deletion mutation group (60 cases). The clinical characteristics, imaging characteristics, expressions of tumor markers and smoking history of patients were compared between the two groups.Results:There was no statistical difference in the gender, age and ethnicity between the exon 21 L858R deletion mutation group and the non-exon 21 L858R deletion mutation group (P values were 0.488, 0.238 and 0.191). There was no statistical difference in the imaging features (including primary tumor site, lobulation, burr, pleural depression and small vacuoles) between the two groups (all P > 0.05). There was no statistical difference in the expressions of tumor markers (including carcinoembryonic antigen, squamous cell carcinoma antigen, neuron-specific enolase, cytokeratin 19 fragment, and gastrin-releasing peptide precursor) between the two groups (all P > 0.05). There were 20 patients (38.5%, 20/52) with smoking history in the exon 21 L858R deletion mutation group, and 4 patients (6.7%, 4/60) with smoking history in the non-exon 21 L858R deletion mutation group, the difference between the two groups was statistically significant (χ 2 = 4.182, P = 0.041). Conclusions:There is no significant difference in clinical characteristics, imaging features and expressions of tumor markers between the patients with exon 21 L858R deletion mutation and the patients without exon 21 L858R deletion mutation. Smoking may be the influencing factor of exon 21 L858R deletion mutation.