Disease Management ; Ductus Arteriosus, Patent ; therapy ; Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; therapy

OBJECTIVE:To evaluate the status quo and tendency of the use of Chinese material medical injection(CMMI) in Nanjing area.METHOD:Chinese material medica injection used in 22 hospital of Nanjing from 2004 to 2006 was analyzed statistically in terms of variety,consumption sum,defined daily dose(DDDs) etc. RESULTS: The consumption sum of CMMI increased year by year,with annual incremental rates of 19.80% and 11.82% respectively.The cardio-cerebral vascular CMMI dominated the first place in terms of consumption sum and DDDs over the 3 years.Lentinan,Shengmai,Shenmai,Puerarin took the lead in consumption sum of single variety.The variety and specification and the number of manufacturers of CMMI increased year by year.CONCLUSION:CMMI plays a key role in clinical treatment,its consumption sum and consumption quantity kept increasing rapidly.

Objective To explore the clinical features of drug abuse mother’ s infants and evaluate the effect of corresponding therapeutics.Methods The clinical data of 54 cases of drug abuse mother’s infants were analyzed. Result During 12 years from 1990 to 2002,54 cases of drug abuse mother’ s infants were treated in our department. In 54 cases,premature was found in 28 cases,LBW in 32 cases,neonatal asphyxia 14 cases, NRDS 12 cases,intracranial hemorrhage 8 cases,congenital anomaly 2 cases. Infants born to addicted mothers have many health problems in the neonatal period Infants born to mothers drug abusing more than 2 years were significantly less mature and lighter than those born to mothers drug abusing less than 2 years( P

Nosocomial infection (NI), a major cause of morbidity and mortality in neonates, is the common challenges faced by health workers worldwide. This paper was to review the epidemiology, major challenges, prevention and control mea-sures of NI in neonatal intensive care unit. The present situation of NI in China and the corresponding countermeasures are also discussed.

Objective To study feasibility in extraction of calcific sign within pulmonary nodules with pattern classification.Meth-ods 49 cases with pulmonary nodules (benign in 16 and malignant in 33)confirmed by pathology or clinical follow-up were included in this study and all cases underwent chest CT examinations.CT images were interpreted double-blind by two associate chief radiolo-gists to draw a conclusion that there were any calcification within pulmonary nodules.Meanwhile,the calcifications in the regions of interest(ROI)on CT images were estimated with extraction of the sign of gray value,geometric and lung markings in ROI,and based on pattern classification algorithm at supporting vector machine(SVM).Results According to the results assessed by senior radiologists for classification within pulmonary nodules,the area under ROC curve was 0.95 which was extracted by automatic pat-tern classification algorithm,the extraction performance was stable(k=1),and was goodness fit with visual observation by doctors (k=0.939).Conclusion The ability of automatic pattern classification in detecting calcification within pulmonary nodules is about the same as that of visual assessment by senior doctors.

Objectives:To explore the pathogenesis of nasal polyposis by examining the expression of transforming growth factor beta (TGF-β) in human inflammatory nasal polyps. 　Methods: Twenty five polyposis patients were selected, tissue TGF-β1～3 from nasal polyps and inferior turbinate mucosa were determined with immunohistochemistry APAAP method .The inferior turbinate mucosa from eight healthy volunteers were selected as control. Six polyp tissues were estimated with western-blot analyses to compare the characteristics of TGF-beta isoforms expression. 　Results: ①The expression of TGF-β1～3 in nasal polyps was significantly higher than that in nasal mucosa, it was undetectable in nasal mucosa from healthy volunteers;②TGF-β1 was the main isoform detected in nasal polyps. 　Conclusions: TGF-β, especially TGF-β1 is strongly expressed in inflammatory nasal polyps and its mucosa. TGF-β could induce modification of epithelium and connective tissue and therefore be involved in the pathogenesis of nasal polyposis.

ObjectiveTo study the relationship between impulsive behavior and alexithymia in pupils in Changsha.Methods 702 pupils were tested with Barratt impulsivity scale (BIS-11) and Toronto alexithymia scale (TAS-20).Results ( 1 ) Male pupils had significantly higher scores of total BIS and non-planning impulsiveness than female (24.47 ± 5.30 vs 23.40 ± 5.59,P ＜ 0.05 ; 62.25 ± 9.46 vs 60.35 ± 9.91,P ＜ 0.05 ).Pupils in elder age group had only significantly higher total score of BIS than pupils in younger age group ( total:62.36 ± 9.69 vs 60.29 ± 9.68,P＜ 0.01 ).(2) Pupils with high impulsivity had significantly higher scores of total and factor scores of alexithymia than pupils with low impulsivity (total:57.43 ± 10.16 vs 46.55 ± 8.77,P ＜0.01 ).The total scores of BIS were positively correlated with total scores of alexithymia ( r =0.40,P ＜ 0.01 ) and factor scores of alexithymia ( r =0.25 ～ 0.33,P ＜ 0.01 ).Stepwise regression analysis revealed that total scores of BIS explained by DIF,DDF and EOT were 18％.ConclusionThere are certain gender and age differences in impulsivity impulsive behavior of 8 to 13 years old pupils.There exist the low to moderate correlations between impulsivity and alexithymia in pupils.

Extracorporeal membrane oxygenation(ECMO) is a kind of extra life support technique that can support cardiac and pulmonary function in a relatively long time.With the application of nitric oxide,pulmonary surfactant and high frequency ventilation,the use of ECMO in neonatal respiratory failure decreased.Although received these treatment,there are some newborn with respiratory failure still required ECMO at last.On this paper,the application of ECMO in neonatal respiratory failure from foreign medical institute was introduced,and compared with the domestic situation,in order to improve the application of ECMO in neonatal respiratory failure.

Objective To analyze the clinical characteristics of Klebsiella pneumoniae infection in preterm infants. Methods Clinical data of 75 preterm infants infected with Klebsiella pneumoniae treated in BaYi Children's Hospital from February 6,2008 to February 10,2010 were retrospectively analyzed.The difference of auxiliary examination between early-onset and late-onset infection group were compared by two independent samples t test.Spearman correlation analysis and non-conditional Logistic regression analysis were used to analyze the high risk factors and the prognostic factors of Klebsiella pneumoniae infection in preterm infants. Results The incidence of Klebsiella pneumoniae infection was 2.8％ (75/2721) in preterm infants,and the mortality rate was 9.3％ (7/75). There were 71 cases of Klebsiella pneumoniae sepsis and 4 cases of Klebsiella pneumoniae pneumonia.Among 75 cases,63 cases were early-onset infection (onset age≤72 h) and 12 were late-onset infection (onset age＞72 h).All patients presented with poor response,heart rate during quiet sleep ＞ 160/min and low oxygen saturation.The mean corpuscular volume and mean corpuscular hemoglobin concentration in early-onset Klebsiella pneunoniae infection cases were higher than those in late-onset neonates [(128.87±24.60) fl vs (113.72±13.54) fl,t=-2.07,P＜0.05and (38.11±2.15) pg vs (36.98±1.05) pg,t=-2.76,P＜0.05].Low birth weight and caesarean section were associated with early-onset Klebsiella pneumoniae sepsis (r=0.250 and -0.240,P＜0.05). The prognosis of Klebsiella pneumoniae infection was associated with hospital stay and duration of premature rupture of membranes (r=0.368 and 0.318,P＜0.05). Conclusions There were no specific clinical manifestations for Klebsiella pneumoniae infection in preterm infants.Preterm infants with low birth weight,long duration of premature rupture of membranes,delivered by caesarean section and received invasive operation are likely to develop Klebsiella pneumoniae infection.

ObjectiveTo investigate the effects of long-term oxygen exposure on the pulmonary microvascular development and the expression of Ephrin-B2 of lungt issue in neonatal mice.MethodsForty-eight 2-day-old Kunming mice were randomly divided into hyperoxia group and air group, with 24 mice in each group. Mice in hyperoxia group were exposed to 70% oxygen to establish a model of bronchopulmonary dysplasia (BPD). Six mice from each group were sacrificed at 3, 7, 14 and 21 days of age, and lung tissue was collected for further test. The lung sections were stained with hematoxylin and eosin for histological evaluation, radial alveolar counts (RAC) and microvessel density (MVD) measurement by CD34 immunohistochemistry. Location and expression of Ephrin-B2 in lung tissue were measured by immunohistochemistry. Ephrin-B2 mRNA and protein levels were detected by fluorescent quantitative reverse transcriptase-polymerase chain reaction and Western blot, respectively. Two independent samplest-test was used for statistical analysis. Results(1) Pathological changes: The pathology of lung tissue in hyperoxia group showed typical BPD-like changes with advancing postnatal age, presenting mainly with simplified alveolar development and decreased microvessel number. Compared with the air group, RAC and MVD were significantly decreased in 14-day-old mice (6.067±0.432 vs 6.950±0.243,t=4.365,P<0.05; 4.133±0.476 vs 4.867±0.472,t=2.680,P<0.01) and 21-day-old mice in the hyperoxia group (8.050±0.362 vs 9.817±0.487,t=7.127,P<0.05; 4.333±0.532 vs 6.017±0.937,t=3.828,P<0.01). (2) Location and expression of Ephrin-B2: Ephrin-B2 was mainly expressed in alveolar epithelial cells, and weakly expressed in alveolar septum. Compared with the air group, the average optical density of Ephrin-B2 was significantly decreased in 7-day-old (0.146±0.013 vs 0.153±0.009), 14-day-old (0.140±0.007 vs 0.161±0.006) and 21-day-old mice in the hyperoxia group (0.138±0.008 vs 0.166±0.009)(t=-2.049,-9.442 and-10.087, allP<0.05). (3) Ephrin-B2 mRNA and protein levels: The Ephrin-B2 mRNA levels in 14-day-old (0.65±0.14 vs 1.05±0.16,t=4.609,P<0.01) and 21-day-old mice (0.57±0.09 vs 1.13±0.18,t=6.816,P<0.01) were significantly lower in hyperoxia group than in the air group. The Ephrin-B2 protein levels were also significantly lower in hyperoxia group than in the air group in 21-day-old mice (0.13±0.03 vs 0.29±0.08,t=4.587,P=0.000).ConclusionsOxygen-induced BPD model mice have simplified alveolar development, reduced MVD and decreased expression of Ephrin-B2 in lung tissue, which may play an important role in the pathogenesis of BPD.