Objective:To evaluate the efficacy and safety of controlled-release dinoprostone pessary(propess) in cervical ripening and intermediate labor induction.Methods:Eighty primiparas with uterogestation and Bishop score ≤6 points and no vaginal delivery taboo were equally divided into a study and a control group.The former received intravaginal administration of propess and the latter intravenous injection of low-dose oxytocin by the randomized control method.Comparisons were made of the Bishop score,delivery and the state of the fetuses and neonates between the two groups.The side effects of propess were observed.Results:Bishop scores were 7.75?2.227 and 6.38?2.862 in the study and the control group respectively 12 hours after the medication,significantly higher than 4.23?1.025 and 4.15?1.099 before the induction(P 0.05).The major side effect of propess was the over-frequent and over-strong contraction of the uterine,which occurred in 2 cases in the study group but subsided after its removal.Conclusion:Dinoprostone pessary,effective,safe and convenient,is obviously superior to oxytocin in cervical ripening and labor induction.

Objective DNA methylation in the pathogenesis of endometriosis is drawing more and more attention from the medical world.This study aimed to evaluate the methylation status of the Homeobox-A10 (HOXA10) gene promoter region and the expression of the HOXA10 protein in endometriosis,and to explore the relationship of endometriosis with the aberrant methylation and expression of HOXA10 and its clinical significance.Methods The methylation-specific PCR(MSP) and the immunohistochemical SP methods were employed to detect both the methylation status of the HOXA10 promoter and its expression in the eutopic and ectopic endometrial tissues from 36 endometriosis patients,with normal endometrial tissue samples from 12 healthy women as controls.Results Hypermethylation of the HOXA10 promoter region was present in 38.9% (14/36) of the ectopic endometrial tissue samples and 25.0% (9/36) of the eutopic endometrial tissue samples,all in endometriosis of stages Ⅲ and Ⅳ,but no hypermethylation was found in the endometrial tissues of the normal controls,with significant differences between the two groups (P

Objective To study the effects of administration of donor apoptotic bone marrow cells via portal vein on the survival time of heart transplantation in rats.Methods Wistar rats were selected as donors and SD rats as recipients, and divided into 4 groups at random. In groups A, B and C, the animals were injected via portal vein with RPMI 1640 0.5 ml 6 as control group, 5?107 donor bone marrow cells and 5?107 apoptotic donor bone marrow cells 6 days before transplantation, respectively , and not subjected to cyclosporin A (CsA). In group D, the animals were injected intra- peritoneally with CsA 5 mg/kg from 3 days before transplantation till 10 days post operation. Bone marrow cells apoptosis was induced by 60 Co? radiation. Heart transplantation models were established in each group. The survival time of donor heart and histological changes were observed. Serum concentrations of IL-10 and TGF-?_1 of recipients and mixed lymphocyte reaction (MLR) were measured. Results The survival time of heart allografts in the group C ( 14.00 ? 0.95 ) was significantly longer than in group A (P

AIM: To establish the quality standard for Yushangling Capsule(Angelica Sinensis,Borneolum Syntheticum,Radix et Rhizoma Notoginseng,etc.). METHODS: Angelica Sinensis and Borneolum Syntheticum were identified by TLC,and the contents of effective components were determined by HPLC. RESULTS: Angelica sinensis,and Borneolum Syntheticum could be identified by TLC.The linear ranges of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 were 0.104 6-1.987 4 ?g,0.407 6-7.744 4 ?g,0.414 8-7.881 2 ?g,respectively,the average recoveries were 99.7%(RSD=0.6%),99.5%(RSD=0.5%),100.1%(RSD=0.7%),respectively CONCLUSION: The method is simple,accurate and high specificity with good repeatability and can be used for quality control of Yushangling Capsule.

Objective To establish the quality standard for Fushenning Tablets. Methods Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae were identified by TLC,and the content of berberine hydrochloride of Cortex Phellodendri Chinensis was determined by HPLC. Results TLC could identify Radix et Rhizoma Rhei,Fructus Gardeniae,Rhizoma Anemarrhenae,Radix Stephaniae Tetrandrae effectively. Berberine Hydrochloride showed a good linear relationship in the range of 0.021 42～ 0.110 20 ? g. The average recovery was 100.6 %(n=6) and RSD was1.4 % . Conclusion This method is simple,accurate,specific and reproducible,and can be used for quality control of Fushenning Tablets.

This paper reports a woman diagnosed with citrullinemia type I (CTLNⅠ) in puerperium who was unfortunately died later. The 28-year-old patient (G1P1) delivered a live girl at 39 +2 gestational weeks and was transferred from a local hospital to Henan Provincial People's Hospital on January 11, 2020, due to "a 3-day paroxysmal confusion accompanied by dizziness 4 days after delivery". Intermittent confusion, elevated blood ammonia, and citrulline concentration, and encephaledema were presented 10 h after delivery, and the patient eventually died of cerebral hernia on the day of self-discharge. Two pathogenic mutations of the ASS1 gene were found by genetic testing, including c.422t>G (p.val141gl; HET) and c.431c>G (p.pro144arg; HET) and confirmed the diagnosis of CTLNⅠ. CTLNⅠ is a life-threatening disease that could be easily overlooked and misdiagnosed and was difficult to treat. It most often occurs in newborns and infants, whilst it is rare during pregnancy and postpartum. The possibility of this disease should be considered in patients with neurological system symptoms and elevated blood ammonia during pregnancy or puerperium.

Objectives:To investigate the level of soluble intracellular adhesion molecule 1 (sICAM 1) concentrations in the serum and peritoneal fluid from the patients with or without endometriosis.To discuss the relationship within sICAM 1 and pelvic endometriosis. Methods:35 serum and 30 peritoneal fluid of patients with endometriosis (test group) and 26 serum and 4 peritoneal fluid without endometriosis (control group) were studied.Soluble intracellular adhesion molecule 1 levels were detected by an enzyme linked immunoassay(ELISA).The results were analysed by T test. Results: The serum concentration of sICAM 1 was significantly higher in test group than controll group. However,There was no significant difference in between two groups. Conclusions: These results suggest that sICAM 1 could play some role in the persistence of endometriotic lesions.

Objective To investigate the relationship of single nucleotide polymorphism (SNP) on FTO gene rs9939609 with metabolism index and obesity in children. Methods One hundred and fifty-three children (age 7-11 year) were recruited in this study, 102 of them were obese and 51 of them were overweight. One hundred and sixty children with normal body weight were recruited as control. Height, weight and biochemical indicator of liver function were measured. PCR and direct sequence were applied to detect the polymorphism of rs9939609, and the frequency of the allele was calculated. Results TT or TA/AA genotype frequency on FTOgene rs9939609 was significantly different among overweight group, obesity group and normal con-trol group (χ2=23.01, P<0.001);TA/AA genotype frequency in overweight and obesity group was significant higher than that in the control group(P<0.014). The frequencies of T and A allele in overweight group, obesity group and control group was 96.25%and 3.75%, 85.29%and 14.71%, 85.78%and 14.22%respectively. There was significant difference of allelic frequency among overweight group, obesity group and control group (χ2=21.72, P<0.001). The frequency of A allele in overweight and obe-sity group was higher than that in control group (P'<0.014). Subjects with TA/AA allele had significantly higher BMI compared with subjects with TT allele. Conclusions rs9939609 of FTO gene is associated with obesity in children, and allele A on this spot may raise BMI and leads to overweight and obesity.

Objective To extract the sulfated polysaccharides from Grateloupia turuturu Yamada and to studyits physico-chemical properties and antiviral activities.Methods The sulfated polysaccharides were extracted from Grateloupia turuturu Yamada by hot water extraction and alcohol precipitation.Two pure polysaccharides were obtained by DEAE-52 cellulose column chromatography.The physico-chemical properties including the composition of the glycoconjugate and the content of the sulfate were analyzed.The antiviral effects of the samples on HSV-1,Cox_(3) and their cytotoxicity to Vero,Hela cell were studied by cytopathic effect(CPE) reduction assay and MTT colorimetric assay.Results The results showed that the glycoconjugate was composed of Gal and slight Glc,Xyl etc.The content of the sulfate group was more than 15%.And the polysaccharides showed good antiviral activities,especially the pure polysaccharides.Their 50% inhibited concentrations(IC_(50))on HSV-1 were(7.81) and 3.91?g?mL~(-1),selectivity index(SI)values were larger than 128 and 256,respectively.Conclusion The sulfated polysaccharides from Grateloupia turuturu Yamada have good antiviral effects on HSV-1 and Cox_(3).

Placenta is a vital organ connecting the developing fetus to the uterine wall for nutrients uptake and gas exchange,as well as fighting against pathogens,and secreting hormones to support pregnancy.For many years,it was believed that fetuses were growing in a germ-free environment.Contrary to this,recent studies demonstrated that a normal placenta harbors a nonpathogenic and diverse population of commensal microorganisms.Moreover,it also revealed that placental microbiome is associated with fetal growth restriction,preterm birth,maternal obesity,gestational diabetes mellitus and preeclampsia.This review summarized recent advances in placental microbiota.