Researches on gene sequencing find that long non-coding RNA (lncRNA)does not encode protein, however,it participates in the biological pathway of gastric cancer and plays a certain role in the process of prognosis and outcome of gastric cancer. With the generation of gene databases and the popularity of computer algorithms,the researches on the prognosis-related lncRNA of gastric cancer are gradually increasing. The bioinformatics study of prognosis-related lncRNA of gastric cancer can provide ideas for experimental researches. It is expected to provide evidence for the diagnosis and treatment of gastric cancer and improve prognosis by exploring more lncRNAs that are related to the prognosis of gastric cancer.

Objective To investigate the pollution of antibiotic resistance genes (ARGs) in the Lhasa River and provide a scientific basis for the safety of drinking water for the regional population and the prevention and control of water environment pollution. Methods A total of five water samples were collected in the Lhasa River in July 2022. Using quantitative real-time polymerase chain reaction (qPCR) assay, 19 types of ARGs, including eight ^“last-resort^” ARGs (LARGs) were detected and analyzed. Statistical analysis was conducted using the SPSS 22.0 software, and Student's t-test was used to compare data between two groups. Results All the 19 ARGs were detected with high frequencies, with the aminoglycoside resistance gene aadA having the highest concentration, followed by the sulfonamide resistance gene sul1 and the macrolide resistance gene ermB. Among the eight LARGs, the carbapenem resistance gene blaOXA-48 had the highest concentration. The absolute and relative concentrations of LARGs were lower than those of common ARGs. There was a statistically significant difference in the absolute concentrations between them, but no significant difference was observed in the relative concentrations. Conclusion Both ^“conventional^” ARGs and LAGRs have been detected in the Lhasa River. Although they are at a relatively low level compared to other domestic waters, in view of the serious adverse effects that ARGs, especially LARGs, may cause, the pollution of ARGs in the Lhasa River should be taken seriously.