Objective To understand the occupational exposure among medical laboratorians in a hospital,and put forward prevention and control measures.Methods Occupational exposure among medical laboratorians in the department of laboratory medicine of a hospital from January 2011 to December 2014 were analyzed statistically.Results A total of 72 cases of occupational exposure occurred among 217 laboratorians during 4 years,occupational exposure rate was 33.18％,the occupational exposure rate in interns was 46.23％,and in laboratorians with working seniority＜ 1 years was 49.56％.Occupational exposure mainly occurred in outpatient blood collection room(accounting for 38.89 ％),the main occupational exposure types were injuries by blood collection needles,skin contaminated by blood and body fluid(76.39 ％).Occupational exposure rates in medical laboratorians in this hospital during 2011-2014 were 53.66％(22/41),41.67％(20/48),26.23％(16/61),and 20.90％(14/67),respectively,which presented a declining tendency(x2 =12.286,P＜0.01).After occupational exposure,only 23 cases were reported according to the requirements of infection management department,the missing report rate was 68.06％ (49/72),correct handling rate was 80.56％ (58/72).Conclusion Laboratorians should strengthen education and training of healthcare-associated infection management,improve safety and prevention consciousness,standardize handling process,improve consciousness of reporting,so as to reduce the occurrence of occupational exposure and post exposure infection.

AIM:To explore the role of sphingosine 1-phosphate (S1P) in the dysfunction of vascular endo-thelial cells exposed to high glucose.METHODS: In human aortic endothelial cells cultured under high-glucose ( 22 mmol/L glucose) medium, nitric oxide ( NO) level, polymorphonuclear neutrophil-endothelial cell adhesion rate, protein level of intercellular adhesion molecule-1 ( ICAM-1) , migration of endothelial cells and Akt/endothelial nitric oxide syn-thase ( eNOS) pathway activation were observed after S1P, sphingosine kinase-1 inhibitor and/or Akt inhibitor treatments. RESULTS:S1P decreased NO level, increased polymorphonuclear neutrophil adhesive rate, enhanced ICAM-1 protein level, and inhibited migration of endothelial cells and activation of Akt/eNOS pathway in endothelial cells cultured under high-glucose condition.Sphingosine kinase-1 inhibitor, which reduced S1P content, significantly improved the above endo-thelial cell function indexes and restored the activation of Akt/eNOS pathway.CONCLUSION: S1P promoted high glu-cose-induced dysfunction of endothelial cells probably by inhibiting the activation of Akt/eNOS signal pathway.Targeting S1P is expected to become one of potential treatment strategies to reduce endothelial cell dysfunction.