1.Clinical observation of antiretroviral therapy in 109 cases of HIV/AIDS patients
Chinese Journal of AIDS & STD 2006;0(04):-
Objective To observe the clinical efficacy of antiretroviral therapy(ART)or highly active antiretroviral therapy(HAART)for HIV/ AIDS patients in Duan county,and its side effects and CD4 levels in the whole blood.Methods One hundred and nine HIV/AIDS cases admitted to our department in the past 2 years were observed in terms of weight change,control of opportunistic infections,improvement of survival and quality of life,side effects and CD4 levels in the whole blood in 3 months after the combined ART.Results Of the 109 cases 78 patients had weight loss before the treatment and all of them gained weight after the treatment,the changes of body weight were significantly different before and after the treatment(P
2.Study the position in bed for the patients who have used heparin sodium after percutaneous coronary intervention therapy
Lizi WANG ; Qiuling LI ; Wei WEI
Chinese Journal of Practical Nursing 2006;0(01):-
Objective To explore the best position in bed for the patients who have under the operation of percutaneous coronary intervention (PCI) and then used heparin sodium within 24 hours. Methods The 159 patients were divided into two groups randomly:the experimental group and the control group. After the PCI, the subjects in these two groups will rest in bed by two ways. Using McGill′s present Pain Index to evaluate hematoma, using the PPI to evaluate the pain of back, using the STAI to evaluate the condition of anxiety, write the number of patient who have anuresises. Results There are no significant difference between two groups in wound blooding, while the difference of back pain, anxiety and the anuresises are significant between two groups. Conclusion The position of half-lie is safe and comfortable for the patients who have under the operation of PCI.
3.Protective Effects of Ethanol Extract of Radix et Rhizoma Gentianae on Acute Lung Injury
Zhongwei MA ; Qiuling WANG ; Peiyu WEI
China Pharmacy 2001;0(11):-
OBJECTIVE: To investigate the protective effects of the ethanol extract of Radix et Rhizoma Gentianae on acute lung injury (ALI). METHODS: 48 NIH male mice were randomized into blank group (normal saline), model group (normal saline), dexamethasone acetate group (0.005 g?kg-1), ethanol extract of Radix et Rhizoma Gentianae groups (high-dosage of 10 g?kg-1, medium dosage of 5 g?kg-1 and low dosage of 2.5 g?kg-1). The groups were administered via i.g gtt once a day for five days. ALI model was induced by intratracheal injection of lipopolysaccharide (LPS, 2.5 mg?kg-1) after last treatment. ELISA method was applied to determine the content of prostaglandin E2 (PGE2), interleukin-10 (IL-10) and cycloxygenase-2(COX-2) at 24 h after modeling. RESULTS: As compared with model group, the content of PGE2 and COX-2 in ethanol extract of Radix et Rhizoma Gentianae groups were decreased significantly (P
4.Effect of astragalus polysaccharides on the cellular immune function in rats with sciatic nerve Wallerian degeneration
Qiuling SANG ; Biao LIU ; Zhuang WEI ; Weitian YIN
Chinese Journal of Immunology 1985;0(02):-
Objective:To explore the effect of astragalus polysaccharides on the cellular immune functions in rats with sciatic nerve Wallerian degeneration.Methods:10 female Wister rats were established as the sciatic nerve injury model,which were randomly divided into astragalus polysaccharides group and control group.Then 20 mg/kg of astragalus polysaccharides were peritoneal injected every postoperative day in experimental group for 7 days and the same volume of saline for the control group.The content of IL-1? in serum and in supernatants of spleenocytes and macrophages was measured by Sandwich ELISA.Results:The proliferation ability of splenic T cells and macrophages in astragalus polysaccharides group was higher than that of control group(P0.05).Conclusion:Astragalus polysaccharides induces the cellular immuno-regulation in sciatic nerve Wallerian degeneration rats and by this way to promote nerve regeneration.
5.Clinical research of the level changes of blood lipids,liver function and estrogen in pregnancy patients with intrahepatic cholestasis
Jiwang DENG ; Wei ZHAO ; Yanling CAI ; Jin FANG ; Zhaodi LIU ; Qiuling YE
International Journal of Laboratory Medicine 2014;(22):3017-3018
Objective To explore the predictive values of the levels of blood lipids ,liver function and estrogen for the fetal dis‐tress in intrahepatic cholestasis of pregnancy (ICP) patients .Methods The indicators of blood lipids ,liver function and estrogen of mild IPC group ,severe ICP group and control group were detected respectively ,and the results were analyzed .Results The levels of triglycerides ,total cholesterol and low‐density lipoprotein were positively related to estradiol levels .Multivariate logistic regres‐sion analysis showed that cholyglycine (OR=8 .24 ,P=0 .01) and estradiol(OR=4 .46 ,P=0 .02) were significant for prediction of fetal distress in ICP patients .Conclusion Estradiol and cholyglycine levels may be better indicators for the prediction of fetal dis‐tress in ICP patients .
6.Sequence analysis and identification of a chloroplast matK gene in Rhei Rhizoma from different botanical origins.
Xiaoqin ZHANG ; Chunsheng LIU ; Xingli YAN ; Xiaoli CHENG ; Juan LIU ; Qiuling WANG ; Kai LIU ; Shengli WEI
Acta Pharmaceutica Sinica 2013;48(11):1722-8
Rhei Rhizoma is a Chinese medicine with multiple botanical origins. There is a problem to identify it with conventional methods. To compare the characteristics of chloroplast matK gene sequences of different Rheum species and authenticate inspected species, the matK gene sequences of different species from different origins were amplified, cloned, and sequenced. Genomic DNA of Rheum plants was extracted using modified DNA extracted Kit and matK gene sequences were analyzed by ContingExpress, DNAman and MEGA5.0. The length of matK gene sequences of Rheum palmatum, R. tanguticum and R. officinale were 1 518 bp containing 57 variable loci. According to the mutation sites, R. palmatum, R. tanguticum and R. officinale were divided into different genotypes separately. Based on the established method according to the loci 587, 707, 838, we successfully identified the genuine Rheum species from its adulterants.
7.Methodological Study of Puerarin Content in Radix Puerariae by Coulomb Electrochemical Determination
Zhenhua WANG ; Lin SUN ; Zhiqiang WEI ; Jun TIAN ; Qiuling KONG ; Yan PAN
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(02):-
Objective To set up the electrochemical determination method for puerarin in Radix Puerariae.Methods The electrochemical behavior and content determination of puerarin were investigated by using coulomb array detector.Results In phosphate buffer with PH 4.32,puerarin showed good linearity in the range of 0.16 ng~ 162 ng.The regression equation was A=1444.7C+ 88.635,r=0.9998(n=7),the average recovery was 96.88 %,and RSD was 0.08 %.Conclusion This method is simple and rapid,and the result is accurate and reliable.It can be used for the determination of puerarin content and biological samples.
8.Exploitation and application of physiological web-based teaching
Wei SUN ; Li LI ; Guilian LIU ; Hong NIAN ; Xuezhi ZHENG ; Qiuling XU
Chinese Journal of Medical Education Research 2003;0(04):-
Developing web-based teaching is one of the foremost tasks for medical education by using multimedia technology.This paper made a brief analysis on exploitation of physiological web-based teaching system,the advantages of web-based teaching and its application.
9.Application of Inductive Diagram Material Database in Rehabilitation Education
Xueyi ZHENG ; Li LI ; Qiuling XU ; Wei SUN ; Chengyu ZHANG ; Guilian LIU
Chinese Journal of Rehabilitation Theory and Practice 2008;14(9):899-900
Inductive diagram material database is a branch material database to complement the multimedia courseware material database.It has a lot of advantages such as brief,explicit and emphasis characteristics,could enhance teaching efficiency and quality.This paper would discuss the advantage of application of inductive diagram in rehabilitation education.
10.Comparative study of plant morphological characteristics and photosynthetic physiological characteristics of wild and cultivated Paeonia lactiflora.
Qiuling WANG ; Shengli WEI ; Wenquan WANG
China Journal of Chinese Materia Medica 2012;37(1):32-36
OBJECTIVEThe aim of this study was to compare the plant morphological characteristics and photosynthetic physiological characteristics of Paeonia Radix Rubra and Paeonia Radix Alba, and find evidence of whether there are intraspecific variations of Paeonia lactiflora.
METHODWith wild and cultivated P. lactiflora of the roots as asexual reproduction materials for cultivation experiment, morphological index, photosynthetic parameters and photosynthetic pigment content were measured.
RESULTIn the 9 of morphological characteristics between wild and cultivated P. lactiflora, the number of leaf, blade length, the width of leaf and the length of top leaf showed a significant difference. The variances within wild P. lactiflora group were big than those within cultivated P. lactiflora in 8 of morphological characteristics. In photosynthetic physiological characteristics, wild P. lactiflora were significantly higher than cultivated P. lactiflora in Max. net photosynthetic rate (P(max)), light compensation point (LCP), light saturation point(LSP), CO2 saturation point (CSP) and CO2 saturated P(n) (P(m)). And significantly lower than cultivated P. lactiflora in maximum net photosynthetic rate (P(max)), light compensation point (LCP), light in apparent quantum yield (AQY), CO2 compensation point (CCP), carboxylation efficiency (CE) and photosynthetic pigment content.
CONCLUSIONWild and cultivated P. lactiflora have significant difference in morphological characteristics of leaf and photosynthetic physiological characteristics.
Carbon Dioxide ; metabolism ; Light ; Paeonia ; anatomy & histology ; growth & development ; metabolism ; radiation effects ; Photosynthesis ; radiation effects ; Plant Leaves ; anatomy & histology ; growth & development ; metabolism ; radiation effects