Objective Disorder of intestinal barrier function is associated with the non-alcohol fatty liver disease ( NAFLD) . The present paper aimed to explore the intestinal barrier function in the rat model of NAFLD . Methods Sixteen SD rats were ran-domly divided into an NAFLD and a normal control group of equal number .The NAFLD models were constructed by high-fat feeding . HE staining was used for pathologic examination of the liver , the levels of TNF-α, IL-1, and endotoxin (ET ) were measured by ELISA and the limulus reagent method , and the expressions of intestinal ZO-1 and Occluding were determined by real time PCR . Resu lts Compared with the normal controls , the NAFLD rats showed typical hepatic lipid deposition , with significantly increased levels of serum TNF-αand IL-1 and plasma ET and decreased expressions of intestinal ZO-1 and Occluding (P<0.05). Conclusion Intestinal barrier function is decreased in NAFLD rats .

To investigate haplotype distribution of the Y-chromosome STRs (DYS19, DYS390 and DYS389 locus) in Han population in Guangdong area. The STRs were typed by poplymerase chain reaction followed by discontinuous PAGE system. Among 130 unrelated males, 81 different haplotypes were observed, 52 out of them were found only one time. The haplotype genetic diversity, discrimination power and non-father exclusion chance are 0. 9989, 0. 9824, 0. 9824, respectively. The high informative haplotypes make these STRs useful for the forensic individual identification and paternity testing.

Objective To study the genetic polymorphisms of 11 Y chromosome specific STR loci in Guangzhou Han population. Method The DNA extracted from blood samples of unrelated individuals in Han population living in Guangzhou were amplified by PCR. The PCR products were analyzed by using PAGE. Results 3-5 alleles were detected in 11 Y - STR loci respectively in Han population in Guangzhou. The minimum GD value was 0.3037 (DYS434), while the maximum GD value was 0.8455 (DYS390) . Conclusion The 11 Y - specific STR loci are highly polymorphic and are suitable for personal identification and paternity testing.

Objective To investigate the polymorphism of DXS6854 locus in Guangdong Han population. Methods The DXS6854 locus was analyzed by PCR following polyacrylamide gel electrophoresis and silver staining. Results Among 189 females and 230 males from Guangdong Han population, 8 alleles were observed with frequencies ranging from 0.0026 to 0.4522. Exact tests demonstrated genotype frequencies in females had no departure from Hardy- Weinberg equilibrium. The discrimination powers for female and male original samples were 0.8633 and 0.7012, respectively. When both mother and her daughter were tested, the probability of excluding a random man as a father was 0.6712. Conclusion The DXS6854 locus is appropriate for individual identification and paternity testing involving a female child.

【Objective】 To understand the polymorphism of D13S63 1 in Guangdong Han population. 【Method】 Short tandem repeat (STR) locus D13S63 1 was analyzed by means of polymerase chain reaction, following discontinuous el ectrophoresis system. 【Result】 Among 227 unrelated individuals from Guangdong Han population, 6 alleles rang from 197～217 bp could be observed. The most com mon allele with a frequency 0.2907 is allele 201 bp, the rare allele is allele 197 bp with a frequency 0.0903. The heterozygosity, the power of discrimination and the exclusion chance in paternity case were 0.7885，0.9231, 0.5543, respect ively. Segregation studies reveal that D13S631 inherit in Medel's Law. 【Conclus ion】 The result shows that locus D13S631 is highly informative and suitable fo r forensic application.

Objective To observe the effect of Sijunzi Decoction(SD) and Huangqi Sijunzi Decoction(HSD) on mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver in rats with spleen asthenia,thus to reveal the pathogenesis mechanism of spleen asthenia from energy metabolism,and to clarify the therapeutic mechanism of spleen-invigorating and Qi-tonifying herbs.Methods Forty SD rats were randomly divided into 4 groups:normal control group,spleen-asthenia model group,SD(10.5 g/kg) group and HSD(18 g/kg) group,10 rats in each group.Except that the normal control group,the rats in the other groups were fed with half-full diet once every other day and with gastic gavage of Xiaochengqi Decoction 60 g/kg every day to establish animal model of spleen asthenia.After 15 days,the model rats were administered the corresponding drugs according to the experimental design for 4 continuous weeks.The changes of mitochondrial succinate dehydrogenase,cytochrome oxidase activity of liver were measured,and the general status of the rats were observed.Results Compared with the normal control group,the level of mitochondrial succinate dehydrogenase and cytochrome oxidase activity in liver of spleen asthenia group were decreased obviously(P

Objective To discuss the probability of siblings identification by autosomal short tandem repeat (STR). Methods 150 pairs of full siblings and 150 pairs of unrelated individuals were genotyped by the 15 STR loci of Power Plex?6 system. Paternity index of full siblings (Pips) and paternity probability of full siblings (WFS) were calculated with the method of ITO. WFS and allelic matching of the two groups were compared, and three sorts of allelic matching in each pair were tested by chi-square test. Results Wps of 100 pairs of siblings (66.67%) were more than 0.9995; WFS of unrelated individuals were less than 0.8, and that of 100 pairs (66.67%) were less than 0.27. The locus number of the entire-same ranged from 1 to 10, averaged 5.49 in siblings, while in unrelated individuals ranged from 0 to 5 and averaged 1.33; that of the entire-different ranged from 0 to 6, averaged 1.66 in siblings but ranged from 2 to 11 and averaged 6.57 in unrelated individuals; that of the half-same ranged from 3 to 13, averaged 7.85 in siblings, and ranged from 1 to 13, averaged 7.11 in unrelated individuals. By chi-square test, there was significant difference (P0.05) in the half-same between the two groups. Conclusion It is effective to identify siblings by Power-Plex?6 system. Testing of 15 STR loci of PowerPlex?6 system, it suggested that the pair were unrelated individuals when the locus number of the entirely-different is not less than 6 or that of the entirely-same is zero, and that the pair were siblings when the locus number of entirely-different is not more than 1 or that of the entirely-same is not less than 6.

Objective Estimate the paternity exclusion probability for 15 short tandem repeats (CODIS set plus Penta D and Penta E) in duo paternity tests. Methods 644 random individuals were paired to con-struct 207046 fictitious duo families. Cases of non-exclusion or with only one exclusionarylocus were count-ed. Results 27 cases could not be excluded by the 15 STR loci. The combined probability of exclusion was 0.999869. Number of cases showing only one exclusionary locus was 384 (0.185%). Conclusion To con-firm relationship in duo cases with only 15 STRs should be careful. Extensive genetic markers is needed to discriminate one locus exclusion or mutation.

Objective:To explore the effect of astragalus polysaccharides on the cellular immune functions in rats with sciatic nerve Wallerian degeneration.Methods:10 female Wister rats were established as the sciatic nerve injury model,which were randomly divided into astragalus polysaccharides group and control group.Then 20 mg/kg of astragalus polysaccharides were peritoneal injected every postoperative day in experimental group for 7 days and the same volume of saline for the control group.The content of IL-1? in serum and in supernatants of spleenocytes and macrophages was measured by Sandwich ELISA.Results:The proliferation ability of splenic T cells and macrophages in astragalus polysaccharides group was higher than that of control group(P0.05).Conclusion:Astragalus polysaccharides induces the cellular immuno-regulation in sciatic nerve Wallerian degeneration rats and by this way to promote nerve regeneration.

Nonalcoholic fatty liver disease (NAFLD) is the most common risk factor for diabetes mellitus and cerebrovascular and cardiovascular diseases. The prevalence of NAFLD is increasing rapidly with the improvement in living. Microsome triglyceride transfer protein (MTP) is a key enzyme for outward transport of liver lipids, and the increase in MTP promoter methylation is an important factor for liver lipid deposition in NAFLD. Traditional Chinese medicine believes that “spleen Qi transfers essence”, and the lipid transport function of MTP may be one of the manifestations of “spleen Qi transfers essence”. Clinical studies have shown that the spleen-strengthening and dampness-removing principle is of great importance in the treatment of NAFLD. By reviewing related articles, this article points out that the spleen-strengthening and dampness-removing therapy can improve NAFLD by regulating MTP promoter methylation, increasing the level of hepatic MTP, promoting the outflow of liver lipids, and alleviating lipid deposition.